2018
DOI: 10.1002/ange.201812111
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Autonomic Behaviors in Lipase‐Active Oil Droplets

Abstract: Developing self-fueled micro-reactor droplets with programmable autonomic behaviors provides as tep towards smart liquid dispersions comprising motile microscale objects. Herein, we prepare aqueous suspensions of lipase-coated oil globules comprising am ixture of at riglyceride substrate (tributyrin, 1,2,3-tributylglycerol) and al ow-density oil (polydimethylsiloxane,P DMS) and describe ar ange of active behaviors based on controlled enzyme-mediated consumption of individual droplets under non-equilibrium cond… Show more

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Cited by 30 publications
(21 citation statements)
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“…The motion was produced by the protocell buoyancy mechanism between lipasecoated lipid PDMS droplets and the loaded tributyrin substrate when the temperature was increased to above 17 • C, producing glycerol and butyric acid. New protocell strategies for the specific chemical and temperature gradients can generate motility-and buoyancy-based physical sorting [319].…”
Section: Propelled By Enzymesmentioning
confidence: 99%
“…The motion was produced by the protocell buoyancy mechanism between lipasecoated lipid PDMS droplets and the loaded tributyrin substrate when the temperature was increased to above 17 • C, producing glycerol and butyric acid. New protocell strategies for the specific chemical and temperature gradients can generate motility-and buoyancy-based physical sorting [319].…”
Section: Propelled By Enzymesmentioning
confidence: 99%
“…When LNMs (50 mgmL À1 )w ere added to aP BS solution containing tributyrin droplets in suspension ( Figure S6), the LNMs exhibited typical Brownian motion with D e = 0.78 AE 0.043 mm 2 s À1 (Figure 3a-d). When the LNMs happened to reach the surface of the oil droplets, they were confined at the oil-water interface because of the inherent amphiphility of lipase, [31] but still maintained ad iffusion coefficient of D e = 0.174 AE 0.038 mm 2 s À1 (Figure S7), which is significantly smaller than that of Brownian motion in bulk solution. When the LNMs happened to reach the surface of the oil droplets, they were confined at the oil-water interface because of the inherent amphiphility of lipase, [31] but still maintained ad iffusion coefficient of D e = 0.174 AE 0.038 mm 2 s À1 (Figure S7), which is significantly smaller than that of Brownian motion in bulk solution.…”
mentioning
confidence: 99%
“…This suggests that low tributyrin concentrations (0.133 mg mL À1 ,s aturated concentration) do not induce enhanced Brownian diffusion of LNMs.A fter adding fuel (triacetin, 10 mm), the LNMs moved more rapidly, resulting in D e = 1.096 AE 0.085 mm 2 s À1 (Figure 3b-d). When the LNMs happened to reach the surface of the oil droplets, they were confined at the oil-water interface because of the inherent amphiphility of lipase, [31] but still maintained ad iffusion coefficient of D e = 0.174 AE 0.038 mm 2 s À1 ( Figure S7), which is significantly smaller than that of Brownian motion in bulk solution. Thec onfinement of LNMs to the oil droplets surface is shown in Figures 3e,f and S8, where the interfacial catalysis between lipase and tributyrin occurred.…”
Section: Angewandte Chemiementioning
confidence: 99%