Lipase‐Powered Mesoporous Silica Nanomotors for Triglyceride Degradation

Abstract

We report lipase-based nanomotors that are capable of enhanced Brownian motion over long periods of time in triglyceride solution and of degrading triglyceride droplets that mimic "blood lipids". We achieved about 40 min of enhanced diffusion of lipase-modified mesoporous silica nanoparticles (MSNPs) through abiocatalytic reaction between lipase and its corresponding water-soluble oil substrate (triacetin) as fuel, which resulted in an enhanced diffusion coefficient (ca. 50 % increase) at low triacetin concentration (< 10 mm). Lipase not only serves as the power engine but also as ah ighly efficient cleaner for the triglyceride droplets (e.g., tributyrin) in PBS solution, which could yield potential biomedical applications, for example,f or dealing with diseases related to the accumulation of triglycerides,o rf or environmental remediation, for example,for the degradation of oil spills.

From Passive Inorganic Oxides to Active Matters of Micro/Nanomotors

“…For instance, mSiO 2 micro/nanospheres are anchored with different enzymes to propel in biological media (Figure 2A). [ 16b,22 ] Tubular inorganic oxide‐based micromotors could be constructed by either deposition of inner catalytic inorganic oxide layer for chemical propulsion with assistance of exterior templates, for instance, MnO 2 tubular microrockets, (Figure 2B) or growth of outer functional inorganic oxide layer to convert light into chemical energy, e.g., ZnO/Pt microjets. [ 23 ] In particular, isotropic Cu 2+1 O (Figure 2C) and Fe 2 O 3 micro/nanomotors have been mass produced through simple solution‐based method without creating structure asymmetry.…”

“…The quantification of enzyme molecules explains the different motion behavior between different types of micromotors and assists architecture design of enzyme‐driven micro/nanomotors. In addition to these commonly used enzymes, Sánchez and co‐workers [ 22 ] extended this field by modifying Janus mSiO 2 nanoparticles with lipase, which exhibited good motion performance in dissolvable triglyceride solution (Figure 3D). The anchored lipase serves as dual roles of the power engine and a highly efficient triglyceride cleaner.…”

Enzyme catalysis powered micro/nanomotors for biomedical applications

“…94 Triglyceride, a major constituent of body fat in humans, which is also present in blood for the bidirectional transference of adipose fat and blood glucose from the liver, 95 was recently used as a fuel for powering motors based on lipase, and the study shows that lipase motors can destroy triglyceride droplets while consuming triglyceride. 96 Apart from them, a plethora of fuels still needs to be investigated for powering MNMs.…”

A Practical Guide to Analyzing and Reporting the Movement of Nanoscale Swimmers

“…the linear part) of a nanoswimmer is sampled in the MSD plot (see for example ref. 66,80 ), which can be fitted with the corresponding equation to yield Deff. Extracting the ballistic speed V from such data, on the other hand, is difficult and inappropriate for nanoswimmers.…”