Autonomous operation of 3D DNA walkers in living cells for microRNA imaging

Hu, Hui; Zhou, Fu; Wang, Baojuan; Chang, Xiaofeng; Dai, Tianyue; Tian, Ruifen; Wan, Yifei; Wang, Xiayan; Wang, Guangfeng

doi:10.1039/d0nr06651f

Cited by 34 publications

(14 citation statements)

References 29 publications

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“…18,19 Singlestranded DNA (ssDNA) is an effective structure to program chemical reaction networks because the sequences are tailorable, and hybridization between complementary strands is reversible. 20,21 To further reduce and eliminate the problems associated with inconsistent folding of ssDNA, doublestranded DNA (dsDNA) offers a superior platform to elaborately control chemical reactions. 22 Subsequently, DNA origami nanostructures have emerged as an alternative tool to program chemical reaction networks with nanometer precision and in many cases, absolute control over stoichiometry.…”

Section: Introductionmentioning

confidence: 99%

Bridge DNA guided assembly of nanoparticles to program chemical reaction networks

Zhang

Jin

et al. 2022

Nanoscale

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Section: Introductionmentioning

confidence: 99%

Bridge DNA guided assembly of nanoparticles to program chemical reaction networks

Zhang

Jin

et al. 2022

Nanoscale

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“…In recent years, due to the small size and low abundance of miRNAs in cells, specific and sensitive imaging of cancer-related miRNA has been promising because of the important applications in risk prediction, drug discovery, diagnosis, and disease treatment . Strategies capable of signal amplification are promising to achieve sensitive detection and imaging of target miRNA. − One of the signal amplification methods is to reuse the target miRNA to continuously release signals . Exonuclease III (EXO III) could assist cascade signal amplification by regenerating and recycling the target nucleic acid .…”

Section: Introductionmentioning

confidence: 99%

“…For signal amplification during detection, a DNA walker which could constantly move along a designed DNA track has also been used to achieve sensitive target nucleic acid detection. ,− Particularly, a DNA walker based on DNA nanoflares − is widely used for signal amplification imaging of target miRNA in living cells. − Enzymes such as EXO III or DNAzyme are generally used as an energy source that complements and enhances the strand displacement strategy to enhance the efficiency of DNA walker motion. ,, DNAzyme has sequence dependence, which sometimes limits its applications. EXO III, in contrast, is more versatile.…”

Section: Introductionmentioning

confidence: 99%

Photoactivated DNA Walker Based on DNA Nanoflares for Signal-Amplified MicroRNA Imaging in Single Living Cells

Chen

Duan

et al. 2021

Anal. Chem.

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Specific and sensitive detection and imaging of cancerrelated miRNA in living cells are desirable for cancer diagnosis and treatment. Because of the spatiotemporal variability of miRNA expression level during different cell cycles, signal amplification strategies that can be activated by external stimuli are required to image miRNAs on demand at desired times and selected locations. Herein, we develop a signal amplification strategy termed as the photoactivated DNA walker based on DNA nanoflares, which enables photocontrollable signal amplification imaging of cancer-related miRNA in single living cells. The developed method is achieved via combining photoactivated nucleic acid displacement reaction with the traditional exonuclease III (EXO III)-assisted DNA walker based on DNA nanoflares. This method is capable of on-demand activation of the DNA walker for dictated signal amplification imaging of cancer-related miRNA in single living cells. The developed method was demonstrated as a proof of concept to achieve photoactivated signal amplification imaging of miRNA-21 in single living HeLa cells via selective two-photon irradiation (λ = 740 nm) of single living HeLa cells by using confocal microscopy equipped with a femtosecond laser.

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“…Of these, the DNA walkers show a better signal amplification ability by taking advantage of their efficient mechanical-like movement along a pre-set route. With the rapid development of interface materials, the previous low-dimensional walking behaviors are progressively replaced by the new three-dimensional (3D) modes to obtain higher mobile efficiency . Benefiting from these merits, the application of 3D DNA walker-assisted fluorescent biosensors has been extended from the early in vitro assay to the current bioimaging.…”

mentioning

confidence: 99%

“…With the rapid development of interface materials, the previous lowdimensional walking behaviors are progressively replaced by the new three-dimensional (3D) modes to obtain higher mobile efficiency. 11 Benefiting from these merits, the application of 3D DNA walker-assisted fluorescent biosensors has been extended from the early in vitro assay to the current bioimaging. Despite much progress having been achieved, this attractive sensing option still suffers from the following challenges.…”

mentioning

confidence: 99%

Upconversion Luminescence-Initiated and GSH-Responsive Self-Driven DNA Motor for Automatic Operation in Living Cells and In Vivo

Liu

et al. 2022

Anal. Chem.

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In light of the worthy design flexibility and the good signal amplification capacity, the recently developed DNA motor (especially the DNA walker)-based fluorescent biosensors can offer an admirable choice for realizing bioimaging. However, this attractive biosensing strategy not only has the disadvantage of uncontrollable initiation but also usually demands the supplement of exogenous driving forces. To handle the above obstacles, some rewarding solutions are proposed here. First, on the surface of an 808 nm near-infrared light-excited low-heat upconversion nanoparticle, a special ultraviolet upconversion luminescence-initiated threedimensional (3D) walking behavior is performed by embedding a photocleavage linker into the sensing elements, and such lightcontrolled target recognition can perfectly overcome the pre-triggering of the biosensor during the biological delivery to significantly boost the sensing precision. After that, a peculiar self-driven walking pattern is constructed by employing MnO 2 nanosheets as an additional nanovector to physically absorb the sensing frame, for which the reduction of the widespread glutathione in the biological medium can bring about sufficient self-supplied Mn 2+ to guarantee the walking efficiency. By selecting an underlying next-generation broad-spectrum cancer biomarker (survivin messenger RNA) as the model target, we obtain that the newly formed autonomous 3D DNA motor shows a commendable sensitivity (where the limit of detection is down to 0.51 pM) and even an outstanding specificity for distinguishing single-base mismatching. Beyond this sound assay performance, our sensing approach is capable of working as a powerful imaging platform for accurately operating in various living specimens such as cells and bodies, showing a favorable diagnostic ability for cancer care.

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Autonomous operation of 3D DNA walkers in living cells for microRNA imaging

Abstract: Three dimensional (3D) DNA walkers hold great potential in serving as an ideal candidate for signal transduction and amplification in bio-assays.

Cited by 34 publications

References 29 publications

Bridge DNA guided assembly of nanoparticles to program chemical reaction networks

Bridge DNA guided assembly of nanoparticles to program chemical reaction networks

Photoactivated DNA Walker Based on DNA Nanoflares for Signal-Amplified MicroRNA Imaging in Single Living Cells

Upconversion Luminescence-Initiated and GSH-Responsive Self-Driven DNA Motor for Automatic Operation in Living Cells and In Vivo

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