Autophagy associated cytotoxicity and cellular uptake mechanisms of bismuth nanoparticles in human kidney cells

Liu, Yongming; Zhuang, Jing; Zhang, Xihui; Cong, Yue; Zhu, Ning; Yang, Lijing; Wang, Yong; Chen, Tao; Wang, Yangyun; Zhang, Leshuai W.

doi:10.1016/j.toxlet.2017.04.014

Cited by 47 publications

(41 citation statements)

References 36 publications

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“…However, bismuth is a heavy metal that is toxic to mammalian cells [35]. For example, human embryonic kidney cells exposed to bismuth for 48 h showed a decreased survival rate, and autophagy was detected [36]. Hence, medicinal plants that exhibit antibacterial, anti-inflammatory, antiulcer, and antiapoptotic effects are useful alternative approaches to eradicate H. pylori infection and gastric cancer treatment.…”

Section: Discussionmentioning

confidence: 99%

Ethyl acetate extract of Kaempferia parviflora inhibits Helicobacter pylori-associated mammalian cell inflammation by regulating proinflammatory cytokine expression and leukocyte chemotaxis

Nemidkanam

Kato

Chaichanawongsaroj

2020

BMC Complement Med Ther

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Background: Kaempferia parviflora (KP) has been used in traditional Thai medicine to cure gastrointestinal disorders since ancient times. Helicobacter pylori is an initiating factor in gastric pathogenesis via activation of massive inflammation, the cumulative effect of which leads to gastric disease progression, including gastric carcinogenesis. Accordingly, the effect of a crude ethyl acetate extract of KP (CEAE-KP) on proinflammatory cytokine production and cell chemotaxis was the focus of this study. Methods: The cytotoxicity of CEAE-KP (8-128 μg/ml) on AGS (gastric adenocarcinoma) cells was determined at 6, 12 and 24 h using an MTT assay. The effect of CEAE-KP on H. pylori-induced interleukin (IL)-8 production by AGS cells was evaluated by ELISA and RT-PCR. The effect of CEAE-KP on monocyte and neutrophil chemotaxis to H. pylori soluble protein (sHP) and IL-8, respectively, was determined using a Boyden chamber assay with THP-1 or HL-60 cells. Results: CEAE-KP reduced AGS cell viability in a concentration-and time-dependent manner, but at 8-16 μg/ml, it was not cytotoxic after 6-24 h of exposure. Coculture of AGS cells with CEAE-KP at a noncytotoxic concentration of 16 μg/ml and H. pylori reduced IL-8 secretion by~60% at 12 h, which was consistent with the decreased level of mRNA expression, and inhibited neutrophil chemotaxis to IL-8. sHP (100 ng/ml) induced marked monocyte chemoattraction, and this was decreased by~60% by CEAE-KP. Conclusion: CEAE-KP might serve as a potent alternative medicine to ameliorate the inflammation mediated by H. pylori infection.

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Section: Discussionmentioning

confidence: 99%

Ethyl acetate extract of Kaempferia parviflora inhibits Helicobacter pylori-associated mammalian cell inflammation by regulating proinflammatory cytokine expression and leukocyte chemotaxis

Nemidkanam

Kato

Chaichanawongsaroj

2020

BMC Complement Med Ther

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“…As shown, all theseN Ms have exhibited non-toxicity against both cancer and non-cancer cells at doses ranging from 100 to 10 000 mgmL À1 .H owever, Wang et al [25] have found that during the therapy with Bi 2 S 3 NPs, urea and creatininel evelsa re enhanced during the first 3d ays after administration but decreased to normall evel after one week, indicating an acute injury of kidney.T oe xplore the associated mechanism for nephrotoxicity,L iu et al have compared the cytotoxicity of Bi 2 S 3 NPs against various cell lines, including human embryonic kidney 293 cells (HEK293), hepatocarcinoma (HepG2), umbilical vein endothelial cells (HUVEC), and lung adenocarcinoma A549 cells, and found that the cytotoxicity of Bi 2 S 3 NPs are more specific to kidney cells and the toxicitye ffect is ascribed to the release of Bi ions. [95] They also found that the expression of microtubule associated protein light chain 3( LC3) was higheri nB i 2 S 3 NP-treated HEK293c ells, indicating autophagy associated mechanismso fb ismuth cytotoxicity (Figure 12). Rabin et al [9a] have also mentioned that Bi ions exhibit more toxicity than Bi 2 S 3 NPs, as demonstrated by the median lethal dose (LD 50 )a gainst U937 macrophages (100 mm for Bi 2 S 3 NPs and 8mm for Bi ions) and HepG2 hepatocytes (114 mm for Bi 2 S 3 NPs and 5mm for Bi ions).…”

Section: Safety Of Bi-based Nmsmentioning

confidence: 93%

Novel Bismuth‐Based Nanomaterials Used for Cancer Diagnosis and Therapy

Cheng

Zhang

2018

Chemistry A European J

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Theranostic nanomaterials (NMs) have gained increasing attention for their simultaneous performance of diagnosis and therapy. Bi-based NMs hold great potential as theranostic platforms based on their X-ray sensitive capability, near-infrared driven semiconductor properties, and distinctive structures, which facilitate the computed tomography (CT) imaging, photoacoustic (PA) imaging, radiation therapy, and phototherapy. The sophisticated design in composition, structure, and surface fabrication of Bi-based NMs can endow these NMs with more modalities in cancer diagnosis and therapy. In this Minireview, we focus on the recent advances in Bi-based theranostic NMs. A series of unique structures and functions as well as the underlying property-activity relationship of Bi-based NMs are showcased to highlight their promising imaging and therapeutic performance. At the end, we propose some challenges for the design and preparation of Bi-based NMs to improve their cancer diagnostic and therapeutic performance.

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“…There is evidence showing that bismuth NPs (BiNPs) can induce the occurrence of autophagy and increase the expression of LC3II in the HEK293 cell line. BiNP-induced renal cytotoxicity may be due to the release of bismuth ions in NPs [75]. Further animal experiments showed that ROS produced by BiNPs may be the main inducement of autophagy, and ROS blockade can reduce autophagy.…”

Section: Inflammation Induced By Enmsmentioning

confidence: 95%

Mechanistic Understanding of the Engineered Nanomaterial-Induced Toxicity on Kidney

Zhao

Zhan

et al. 2019

Journal of Nanomaterials

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With the rapid development of nanotechnology, engineered nanomaterials (ENMs) have been applied in many fields, such as food industry, biomedicine, and so on. However, the study on the health and safety implications of ENMs is still insufficient. Previous studies have shown that nanoparticles under acute or chronic exposure could be transported and accumulated in various organs and tissues, resulting in adverse effects or systemic toxicity. Among these, the kidney is one of the main organs that exposed ENMs will target through different routes. One of the important functions of the kidney is to discharge metabolic wastes and exogenous substances from the blood circulation of the whole body. During ENM exposure, the kidney may become vulnerable to toxicity. Studies have suggested that nanoparticles exposed to the kidney could provoke glomerular swelling, basilar membrane thickening, degeneration, and necrosis of renal tubular cells. These adverse effects of nanoparticles on the kidney may be related to their induced oxidative stress, inflammation, autophagy, DNA damage, and ER stress. This review aims to examine current studies on ENM-induced nephrotoxicity, with the focus on elucidating the potential molecular mechanisms of nanoparticle-induced toxicity on the kidney, which will further facilitate the safer design of ENMs and their applications.

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Autophagy associated cytotoxicity and cellular uptake mechanisms of bismuth nanoparticles in human kidney cells

Cited by 47 publications

References 36 publications

Ethyl acetate extract of Kaempferia parviflora inhibits Helicobacter pylori-associated mammalian cell inflammation by regulating proinflammatory cytokine expression and leukocyte chemotaxis

Ethyl acetate extract of Kaempferia parviflora inhibits Helicobacter pylori-associated mammalian cell inflammation by regulating proinflammatory cytokine expression and leukocyte chemotaxis

Novel Bismuth‐Based Nanomaterials Used for Cancer Diagnosis and Therapy

Mechanistic Understanding of the Engineered Nanomaterial-Induced Toxicity on Kidney

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