Autoradiographic Investigation of Effects of High-Dose Colchicine on Dentinogenesis in Rat Incisors

Nogueira, T. O.; Zorn, Telma Maria Tenório; Stene, Torbjörn; Koppang, Hanna Strömme

doi:10.1159/000146613

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…Microtubules, which are known to play a major role in the polarization of epithelial cells (Bacallao et al, 1989;Rodriguez-Boulan and Salas, 1989), may also play a similar role in odontoblasts (Ruch et al, 1975;Bronckers et al, 1988;Nogueira et al, 1988). The unusual single sighting of a centriole toward the pulpal pole, as opposed to the dentinal one, was made in a cell which contained a much higher than average percentage of the endoplasmic reticulum which extended toward the pulp (see Fig.…”

Section: Discussionmentioning

confidence: 99%

The Crown Odontoblasts of Rat Molars from Primary Dentinogenesis to Complete Eruption

Romagnoli¹,

Mancini²,

Galeotti³

et al. 1990

J Dent Res

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The involution of crown odontoblasts after primary dentinogenesis in teeth of limited eruption is discussed. The odontoblasts of rat first lower molars were analyzed morphometrically from the tenth day to the 40th day of age, i.e., from the late phase of primary dentinogenesis to complete eruption. All the organelles underwent atrophy, but at different rates. In particular, the membranes of the endoplasmic reticulum decreased progressively in surface area from day 10 to day 40, whereas those of the Golgi apparatus decreased significantly between day 10 and day 14, and then remained practically unchanged in size. The volume of the lysosome compartment never increased beyond that during primary dentinogenesis. The profile length of the endoplasmic reticulum in each observed cell section was taken as an estimate of secretory activity. At day 40, this organelle was smaller in approximately 95% of the cells than it had been in any cell at day 10. These results suggest that cell atrophy may occur without any increase in the degradation processes of the cytoplasmic components and that the organelles along the secretory pathway may have independent regulatory systems. In the odontoblasts, as in several types of secretory epithelial cells, only a small fraction of the cells is engaged in appreciable secretory activity. This occurs, however, when the overall activity of the same cell population is relatively low.

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Section: Discussionmentioning

confidence: 99%

The Crown Odontoblasts of Rat Molars from Primary Dentinogenesis to Complete Eruption

Romagnoli¹,

Mancini²,

Galeotti³

et al. 1990

J Dent Res

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Dentinogenesis

Linde

Goldberg

1993

Critical Reviews in Oral Biology & Medicine

428

333

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The formation of dentin, dentinogenesis, comprises a sophisticated interplay between several factors in the tissue, cellular as well as extracellular. Dentin may be regarded as a calcified connective tissue. In this respect, as well as in its mode of formation, it is closely related to bone. Using dentinogenesis as an experimental model to study biomineralization provides several practical advantages, and the results may be extrapolated to understand similar processes in other tissues, primarily bone. After describing dentin structure and composition, this review discusses items such as the morphology of dentinogenesis; the dentinogenically active odontoblast, transport, and concentrations of mineral ions; the constituents of the dentin organic matrix; and the presumed mechanisms involved in mineral formation.

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Cytochemical localization of calcium and Ca2+,Mg2(+)-adenosine triphosphatase in colchicine-altered rat incisor ameloblasts.

Eisenmann

Salama²,

Zaki³

et al. 1990

J Histochem Cytochem.

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Colchicine is known to affect secretory, transport, and degradative functions of ameloblasts. The effects of colchicine on membrane-associated calcium and Ca2+,Mg2(+)-ATPase in secretory and maturation ameloblasts were investigated cytochemically. The pyroantimonate (PPA) method was used for localizing calcium and a modified Wachstein-Meisel medium was used to localize Ca2+,Mg2(+)-ATPase. Sections representing secretory and early maturation stages were examined by transmission electron microscopy. Morphological changes induced by colchicine included dislocated organelles and other well-established reactions to such anti-microtubule drugs. Calcium pyroantimonate (Ca-PA) deposits in most ameloblast types were markedly reduced, with the greater reduction occurring in those cells more severely altered morphologically. However, the cell membranes of both control and experimental smooth-ended maturation ameloblasts were essentially devoid of Ca-PA. The normal distribution and intensity of Ca2+,Mg2(+)-ATPase was not affected by colchicine. Because the observed reduction of membrane-associated calcium is apparently not mediated by Ca2+,Mg2(+)-ATPase in this case, other aspects of the calcium regulating system of ameloblasts are apparently targeted by colchicine.

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Autoradiographic Investigation of Effects of High-Dose Colchicine on Dentinogenesis in Rat Incisors

Cited by 3 publications

References 14 publications

The Crown Odontoblasts of Rat Molars from Primary Dentinogenesis to Complete Eruption

The Crown Odontoblasts of Rat Molars from Primary Dentinogenesis to Complete Eruption

Dentinogenesis

Cytochemical localization of calcium and Ca2+,Mg2(+)-adenosine triphosphatase in colchicine-altered rat incisor ameloblasts.

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