Autoradiographic Localization of Muscarinic Receptors in the Gut

Buckley, N J

doi:10.1007/978-3-642-70668-4_1

Cited by 4 publications

(2 citation statements)

References 47 publications

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“…Pirenzepine has a higher affinity for Ml-muscarinic receptors than for M2-receptors, whereas atropine or hyoscine do not discriminate between both subtypes (for review see Birdsall & Hulme, 1985). M l-receptors have been detected on guinea-pig myenteric neurones in functional (Kilbinger & Nafziger, 1985; and autoradiographic (Buckley, 1985) studies, but it is not known whether these receptors play a role in intestinal motility.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of guinea‐pig intestinal peristalsis by blockade of muscarinic M₁‐receptors

Schwörer

Kilbinger

1988

British J Pharmacology

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1 The effects of pirenzepine and hyoscine on the peristaltic reflex were investigated in the guineapig isolated small intestine. Peristalsis was induced by raising the intraluminal pressure and the volume of fluid propelled was taken as a measure of the efficiency of peristaltic activity. 2 Low concentrations of pirenzepine (0.1-1 nM) and of hyoscine (0.01 nM) significantly enhanced peristalsis, whereas larger concentrations of both drugs caused inhibition. Pirenzepine was about 6 times less potent than hyoscine in increasing peristalsis, but was about 100 times less potent in inhibiting it.3 Neither tolazoline (1 yM) nor naloxone (0.3 pM) affected the stimulatory action of pirenzepine on peristalsis. 4 Bicuculline increased the efficiency of peristalsis at concentrations of 1 gM and 10M; at I0nM, bicuculline reduced significantly the increase of peristalsis by pirenzepine. y-Aminobutyric acid (GABA) did not affect peristaltic activity, but the stimulatory effect of pirenzepine was abolished in the presence of 100 yM GABA.5 The results indicate that activation of neuronal Ml-receptors causes inhibition of small intestinal peristalsis. Bicuculline-sensitive 'GABAergic' synapses are probably involved in this inhibition.

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Section: Introductionmentioning

confidence: 99%

Enhancement of guinea‐pig intestinal peristalsis by blockade of muscarinic M₁‐receptors

Schwörer

Kilbinger

1988

British J Pharmacology

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“…The brief duration of pirenzepine action which we observed is in agreement with the results obtained from studies of [3H]pirenzepine dissociation rates; Schudt, Auriga, Birdsall & Boer (1988) demonstrated that this was 8 min in the case of membranes prepared from rat forebrain. Autoradiographic studies have shown that M1 receptors are present in the enteric nervous system since tritiated pirenzepine binds selectively on intramural ganglia in guinea-pig ileum (Buckley & Burnstock, 1984). On the basis of intracellular recordings, it was demonstrated that M1 receptors are present on a number of neural perikarya in the myenteric plexus of guinea-pig small intestine where they mediate slow long-lasting EPSPs (North et al 1985).…”

Section: Discussionmentioning

confidence: 99%

Role of M1 muscarinic receptors in the parasympathetic control of colonic motility in cats and rabbits.

Blanquet¹,

Gonella²

1992

The Journal of Physiology

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SUMMARY1. The effects of pirenzepine (a selective blocking agent of M1 muscarinic receptors) were studied on excitatory junction potentials (EJPs) and inhibitory junction potentials (IJPs) elicited on colonic smooth muscle by stimulation of efferent parasympathetic nerve fibres in anaesthetized cats and rabbits.2. Pirenzepine (25,tg kg-' to 0-2 mg kg-', i.v.) decreased the amplitude of EJPs or abolished them. In pirenzepine, parasympathetic stimulation elicited IJPs in most cases.3. In both species, pirenzepine initiated spontaneous IJPs, indicating an increase in the activity of the non-adrenergic, non-cholinergic (NANC) inhibitory neurones.4. The results suggest that Ml muscarinic receptors are involved in synaptic transmission within intramural plexuses, at interneuronal synapses in the parasympathetic excitatory pathway to colonic smooth muscle, but are not involved in the pathway to the NANC inhibitory neurones. The facilitation of IJPs by pirenzepine suggests that, under normal physiological conditions, NANC neurones are tonically inhibited by an intramural nervous circuit involving M1 muscarinic receptors.

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Control of Acetylcholine Release and of Intestinal Motility by Subtypes of Muscarine Receptors

Kilbinger

Schwörer

Stein

et al. 1988

Modulation of Synaptic Transmission and Plasticity in Nervous Systems

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Autoradiographic Localization of Muscarinic Receptors in the Gut

Cited by 4 publications

References 47 publications

Enhancement of guinea‐pig intestinal peristalsis by blockade of muscarinic M₁‐receptors

Enhancement of guinea‐pig intestinal peristalsis by blockade of muscarinic M₁‐receptors

Role of M1 muscarinic receptors in the parasympathetic control of colonic motility in cats and rabbits.

Control of Acetylcholine Release and of Intestinal Motility by Subtypes of Muscarine Receptors

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Autoradiographic Localization of Muscarinic Receptors in the Gut

Cited by 4 publications

References 47 publications

Enhancement of guinea‐pig intestinal peristalsis by blockade of muscarinic M1‐receptors

Enhancement of guinea‐pig intestinal peristalsis by blockade of muscarinic M1‐receptors

Role of M1 muscarinic receptors in the parasympathetic control of colonic motility in cats and rabbits.

Control of Acetylcholine Release and of Intestinal Motility by Subtypes of Muscarine Receptors

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Enhancement of guinea‐pig intestinal peristalsis by blockade of muscarinic M₁‐receptors

Enhancement of guinea‐pig intestinal peristalsis by blockade of muscarinic M₁‐receptors