2000
DOI: 10.1002/1096-9861(20000724)423:2<319::aid-cne9>3.0.co;2-c
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Autoradiographic localization of125I[Tyr14]orphanin FQ/nociceptin and125I[Tyr10]orphanin FQ/nociceptin(1-11) binding sites in rat brain

Abstract: The endogenous ligand for the orphan opioid receptor, orphanin FQ/nociceptin (OFQ), has recently been characterized. The OFQ peptide sequence contains paired basic amino acids, suggesting the possibility of posttranslational processing to a peptide containing the first 11 amino acids of the OFQ peptide. This peptide has been reported in the brain and it has a unique pharmacology. In the present study, we compared the autoradiographic distribution of (125)I[Tyr(14)]OFQ and (125)I[Tyr(10)]OFQ(1-11) in coronal ra… Show more

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Cited by 44 publications
(5 citation statements)
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“…Expression of ORL-1 mRNA appears primarily limited to principal neurons in CA1, CA3, and the dentate (Ikeda et al, 1998;Neal et al, 1999b). In contrast, autoradiography shows that binding is highest in dendritic layers (Neal et al, 1999b;Letchworth et al, 2000), suggesting that the receptor protein is transported to dendrites and/or terminals. Thus Noc and its receptors are critically localized to regulate excitatory activity in hippocampal efferents and afferents.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Expression of ORL-1 mRNA appears primarily limited to principal neurons in CA1, CA3, and the dentate (Ikeda et al, 1998;Neal et al, 1999b). In contrast, autoradiography shows that binding is highest in dendritic layers (Neal et al, 1999b;Letchworth et al, 2000), suggesting that the receptor protein is transported to dendrites and/or terminals. Thus Noc and its receptors are critically localized to regulate excitatory activity in hippocampal efferents and afferents.…”
Section: Discussionmentioning
confidence: 99%
“…Among opiate peptides, Noc exhibits the highest homology to dynorphin (Meunier et al, 1995), and ORL-1 may be analogous to the 3 receptor identified pharmacologically (Pan et al, 1998). Noc and ORL-1 are abundantly expressed throughout the hippocampus (Florin et al, 1997;Neal et al, 1999a;Letchworth et al, 2000), where Noc has postsynaptic augmenting actions on K ϩ currents of principal neurons in CA1, CA3, and the dentate (Ikeda et al, 1997;Yu and Xie, 1998;Madamba et al, 1999b;Amano et al, 2000). As with dynorphin, no disinhibitory actions of Noc have been reported in the hippocampus.…”
mentioning
confidence: 99%
“…35 S]GTP-␥-S binding, and in situ hybridization (Shimohira et al, 1997;Sim and Childers, 1997;Ikeda et al, 1998;Neal et al, 1999a;Letchworth et al, 2000).…”
Section: Abstract: Thalamus; Electrophysiology; Patch-clamp; Neuropementioning
confidence: 99%
“…Low to moderate signals for the ORL receptor are evenly distributed in thalamic nuclei (Shimohira et al, 1997;Sim and Childers, 1997;Ikeda et al, 1998;Neal et al, 1999a;Letchworth et al, 2000), suggesting that N/OFQ may act as a transmitter inherent to the intrathalamic circuitry. Despite numerous efforts, including electrical stimulation within the thalamus at different sites using different stimulation protocols, a response sensitive to selective ORL antagonists could not be reliably obtained in the present study (data not shown).…”
Section: Functional Significance Of N/ofq Effects In the Thalamusmentioning
confidence: 99%
“…Whether combined MRF/MFB low intensity stimulation leads to the release of any of these substances within the MRF or other brain areas remains to be determined. Nociceptin and putative nociceptin receptor binding sites have been shown to be present in the MRF, and could therefore act as an anti-opioid substance within this area (Letchworth et al, 2000; Neal et al, 1999). …”
Section: Discussionmentioning
confidence: 99%