Autoradiographic visualization of group III metabotropic glutamate receptors using [<sup>3</sup>H]‐<scp>L</scp>‐2‐amino‐4‐phosphonobutyrate

Hudtloff, Camilla; Thomsen, C.

doi:10.1038/sj.bjp.0701910

Cited by 17 publications

(11 citation statements)

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“…[ 3 H]L-AP4 in vitro receptor autoradiography was performed as described previously (Hudtloff and Thomsen, 1998). Brains from wild-type (6 -8 weeks of age) or age-matched homozygous mGluR4 knock-out mice (Pekhletski et al, 1996) were removed and immediately frozen in powdered dry ice.…”

Section: Methodsmentioning

confidence: 99%

“…However, the overall relative density of high-affinity L-AP4 receptors is not evident from these studies. To this end, [ 3 H]L-AP4 receptor autoradiography has been characterized in rat brain, and [ 3 H]L-AP4 was shown to label a G proteincoupled binding site with a group III mGluR pharmacology (Hudtloff and Thomsen, 1998). Because [ 3 H]L-AP4 binds to all group III mGluRs, [ 3 H]L-AP4 autoradiography in brain tissue likely represents a composite of several mGluR subtypes.…”

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confidence: 99%

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Contribution of Metabotropic Glutamate Receptor mGluR4 to L‐2‐[³H]Amino‐4‐Phosphonobutyrate Binding in Mouse Brain

Thomsen¹,

Hampson²

1999

Journal of Neurochemistry

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3 H]L-AP4 binding were observed in the molecular layer of the cerebellar cortex, the nucleus basalis, the outer layer of the superior colliculus, and the substantia nigra. In mGluR4 knock-out mice, very low levels of binding were observed in these regions. The moderate levels of binding observed with wild-type mice in the molecular layer of the hippocampal dentate gyrus and in the thalamus were absent in mGluR4 knock-out mice. In contrast, the moderate levels observed in most of the cerebral cortex, caudate putamen, and globus pallidus were not different in mGluR4 knock-out mice compared with wild-type. In these regions, mGluR8 is likely to be labeled by [3 H]L-AP4 because mGluR8 is expressed in such brain regions and, like mGluR4, has high affinity for L-AP4. We conclude that mGluR4 contributes substantially to the high-affinity binding site for [ 3 H]L-AP4 in several regions of mouse brain, including cerebellar cortex, nucleus basalis, thalamus, superior colliculus, substantia nigra, and hippocampal dentate gyrus. Key Words: Metabotropic glutamate receptor-L-2-[ 3 H]Amino-4-phosphonobutyric acid receptor autoradiography-mGluR4 knock-out mice-L-2-Amino-4-phosphonobutyrate receptor.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Contribution of Metabotropic Glutamate Receptor mGluR4 to L‐2‐[³H]Amino‐4‐Phosphonobutyrate Binding in Mouse Brain

Thomsen¹,

Hampson²

1999

Journal of Neurochemistry

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“…1996) during visual stimulation. A recent study (Hudtloff & Thomsen 1998) shows that the level of binding for the radiolabelled Group III agonist L‐AP4 in the SSC is very high in comparison with other brain regions. There is a growing body of literature from previous studies which indicates that activation of Group III mGluRs depresses synaptic transmission by reducing glutamate release (see Glaum & Miller 1994 and Cochilla & Alford 1998).…”

Section: Introductionmentioning

confidence: 99%

Physiological role of group III metabotropic glutamate receptors in visually responsive neurons of the rat superficial superior colliculus

Cirone

Salt

2000

Eur J of Neuroscience

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There is evidence from immunohistochemical and in situ hybridization studies for the presence of Group I, II and III metabotropic glutamate receptors (mGluRs) in the rat superficial superior colliculus (SSC). The purpose of this study was to investigate if manipulation of Group III mGluRs affects visual responses in the SSC. Drugs were applied by iontophoresis and single neuron activity was recorded extracellularly. L-AP4 (Group III agonist) resulted in a reduction of visual responses in most neurons, but also a potentiation in others. The effect of L-AP4 is drug- and stereospecific in that application of D-AP4 did not significantly affect visual responses. L-AP4 application also resulted in a potentiation of the response to iontophoretically applied NMDA. The effects of MPPG and CPPG (Group III antagonists) were compared with the effect of L-AP4 in the same neuron and were found to produce the opposite effect to L-AP4. Furthermore, the effect of L-AP4 could be blocked by coapplication of MPPG or CPPG. Presynaptic depression of glutamate release is a possible mechanism by which L-AP4 could reduce visual responses in the SSC whereas the potentiation of visual responses by L-AP4 could be due to a reduction of GABAergic inhibition. The finding that MPPG and CPPG, as well as antagonizing the L-AP4 effect, have a direct effect on visual responses suggests that Group III mGluRs are activated by endogenous transmitter released during visual stimulation.

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“…Activation of these receptors leads to inhibition of protein kinase A activity and cAMP production [4]. A large number of morphological studies have demonstrated expression of most mGluR subtypes in the striatum at variable levels [8,23,25]. The enriched mGluR distribution in this region indicates putative involvements of mGluRs in the regulation of striatal function.…”

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Acute administration of cocaine reduces metabotropic glutamate receptor 8 protein expression in the rat striatum in vivo

Zhang

Parelkar

et al. 2009

Neuroscience Letters

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Metabotropic glutamate receptors (mGluRs) are densely expressed in the limbic system of mammalian brain. Increasing evidence suggests a critical role of mGluRs in the pathogenesis of various mental illnesses, including drug abuse and addiction. In this study, we investigated the effect of psychostimulant cocaine on protein expression of a specific mGluR subtype, mGluR8, in the rat forebrain in vivo. A rabbit antibody against the extracellular N-terminus of mGluR8 was developed to detect changes in mGluR8 proteins in immunoblot assays. With this antibody, we found that acute systemic injection of cocaine reduced mGluR8 protein levels in the striatum. The reduction of mGluR8 proteins was rapid and transient as it was induced 25 min after cocaine injection and returned to the normal level by 6 h. No significant change in mGluR8 protein levels in the prefrontal cortex and the hippocampus was observed following cocaine administration. These data demonstrate that protein expression of mGluR8 is subject to the modulation by dopamine stimulation. Acute exposure to cocaine results in a dynamic and region-specific downregulation of mGluR8 expression in the striatum.

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Autoradiographic visualization of group III metabotropic glutamate receptors using [³H]‐L‐2‐amino‐4‐phosphonobutyrate

Cited by 17 publications

References 32 publications

Contribution of Metabotropic Glutamate Receptor mGluR4 to L‐2‐[³H]Amino‐4‐Phosphonobutyrate Binding in Mouse Brain

Contribution of Metabotropic Glutamate Receptor mGluR4 to L‐2‐[³H]Amino‐4‐Phosphonobutyrate Binding in Mouse Brain

Physiological role of group III metabotropic glutamate receptors in visually responsive neurons of the rat superficial superior colliculus

Acute administration of cocaine reduces metabotropic glutamate receptor 8 protein expression in the rat striatum in vivo

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Autoradiographic visualization of group III metabotropic glutamate receptors using [3H]‐L‐2‐amino‐4‐phosphonobutyrate

Cited by 17 publications

References 32 publications

Contribution of Metabotropic Glutamate Receptor mGluR4 to L‐2‐[3H]Amino‐4‐Phosphonobutyrate Binding in Mouse Brain

Contribution of Metabotropic Glutamate Receptor mGluR4 to L‐2‐[3H]Amino‐4‐Phosphonobutyrate Binding in Mouse Brain

Physiological role of group III metabotropic glutamate receptors in visually responsive neurons of the rat superficial superior colliculus

Acute administration of cocaine reduces metabotropic glutamate receptor 8 protein expression in the rat striatum in vivo

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Autoradiographic visualization of group III metabotropic glutamate receptors using [³H]‐L‐2‐amino‐4‐phosphonobutyrate

Contribution of Metabotropic Glutamate Receptor mGluR4 to L‐2‐[³H]Amino‐4‐Phosphonobutyrate Binding in Mouse Brain

Contribution of Metabotropic Glutamate Receptor mGluR4 to L‐2‐[³H]Amino‐4‐Phosphonobutyrate Binding in Mouse Brain