Autoradiographische Bestimmung der Dauer der DNS-Verdopplung und der Generationszeit beim Ehrlich-Ascitestumor der Maus durch Doppelmarkierung mit 14C- und 3H-Thymidin

Quantification of Entry Into and Exit From the Cell Cycle in Human Lymphocyte Cultures

“…A description of the relative numbers of cells in the cycling compartment distributed from early to late phases of the cell cycle (age distribution) is necessary for theoretical extrapolation of growth kinetics. Published methods for analysis of this parameter alternatively make simplifying but generally inappropriate assumptions (Lennartz & Maurer, 1964), are experimentally elaborate (Sisken & Morasca, 1965) or mathematically awesome (Powell, 1956). The approach presented here makes certain assumptions which introduce only minor errors or are at least conceptually appropriate and has the advantage of experimental and mathematical simplicity.…”

Cell Renewal in the Gastric Mucosa

“…Another procedure to estimate S-phase duration consists in the applica tion of 2 successive pulse labels, the 1st with 3H-thymidine, and the 2nd, after a period equal to or less than G2, with C14-thymidine [38,69]. The cells labelled with 3H alone and those labelled with C14 can be distinguished by using special autoradiographic procedures.…”

Pathology of Experimental Neurogenic Tumors Chemically Induced During Prenatal and Postnatal Life

“…To elucidate some of the dynamic properties of tumor growth beyond the morphological aspect, autoradiographic studies were initiated by Kleihues. Two techniques employing double labeling of ;I specific DNA precursor were used: double labeling with 14C-and :3H-thymidinc (Hilscher & Maurer, 1962: Baserga & Lisco, 1963Wimbler & Quastler, 1963: Lennartz & Maurer, 1964, and double labeling with two different dosc levels of tritiated thymidine (Wegener er a / . , 1964).…”