Autoradiographische Untersuchung �ber den Eiwei�stoffwechsel in den Geweben und Zellen des Auges

Nover, A; Schultze, B.

doi:10.1007/bf00686431

Cited by 21 publications

(3 citation statements)

References 45 publications

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“…During subsequent days, the discrete, radioactive band gradually moved toward the apical end of the cells (Figs. [16][17][18][19][20]. As in the rats and mice, the band neither increased in width nor decreased FmLmES 2-7 Radioautographs of rat photoreceptor cells from animals 8 wk old at the time of injection of methionine-3H.…”

Section: Frogsmentioning

confidence: 99%

“…However, the source of this material is the cell nucleus, which continually supplies newly synthesized RNA to the inner segment (2,29). The rapid incorporation of radioactive amino acids into the inner segment indicates that protein synthesis is confined largely to this RNA-rich compartment of the cell (8,14,19). Droz observed by radioautography that in rat and mouse photoreceptors most of the radioactive protein was displaced from the inner to the outer segments (8).…”

Section: Introductionmentioning

confidence: 99%

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The Renewal of Photoreceptor Cell Outer Segments

Young

1967

The Journal of Cell Biology

1,071

616

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The utilization of methionine-aH by retinal photorcceptor cells has been studied by radioautographic tcchnique in the rat, mouse, and frog. In all three species, the labeled amino acid is conccntratcd initially in the inner segment of the cell. Within 24 hr, the radioactive material is displaced to the base of the outer segment, where it accumulates as a distinct reaction band. The reaction band then gradually moves along the outer segment and ultimately disappears at the apex of the cell, which is in contact with the retinal pigment epithelium. These findings are interpreted to indicate that the photoreceptor ccll outer segment is continually renewed, by the repcated lamellar apposition of material (membranous discs) at the base of the outer scgment, in conjunction with a balanced removal of material at its apex. The outer segment rencwal rate is accelerated in frogs when ambient temperature is raiscd, and is elcvated in both frogs and rats when the intensity of retinal illumination is increased.

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Section: Frogsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Renewal of Photoreceptor Cell Outer Segments

Young

1967

The Journal of Cell Biology

1,071

616

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“…Protein is distributed throughout the photoreceptor cells and is particularly concentrated in the outer segment (7). However, the synthesis of protein is largely restricted to the inner segment, particularly its myoid portion (7,20,27,38). Droz (7) presented the first evidence that renewal of protein involves an intracellular redistribution of that cell constituent.…”

Section: Introductionmentioning

confidence: 99%

The Renewal of Protein in Retinal Rods and Cones

Young

Droz

1968

The Journal of Cell Biology

371

143

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The renewal of protein in retinal rods and cones has been analyzed by quantitative electron microscope radioautography in adult frogs injected with a mixture of radioactive amino acids. Protein synthesis occurs predominantly in the ergastoplasm, localized in the myoid region of the photoreceptor cells. Much of the newly formed protein next flows through the Golgi complex. In rods, a large proportion of the protein then moves past the mitochondria of the ellipsoid segment, passes through the connecting cilium into the outer segment, and is there assembled into membranous discs at the base of that structure. Discs are formed at the rate of 36 per day in red rods and 25 per day in green rods at 22.50 C ambient temperature. In cones, a small proportion of the protein is similarly displaced to the outer segment. However, no new discs are formed. Instead, the protein becomes diffusely distributed throughout the cone outer segment. Low levels of radioactivity have been detected, shortly after injection, in the mitochondria, nucleus, and synaptic bodies of rods and cones. Nevertheless, in these organelles, the renewal process also appears to involve the utilization of protein formed in the ergastoplasm of the myoid.

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Physiological Chemistry of the Eye

Potts¹

1961

Arch Ophthalmol

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Autoradiographische Untersuchung �ber den Eiwei�stoffwechsel in den Geweben und Zellen des Auges

Cited by 21 publications

References 45 publications

The Renewal of Photoreceptor Cell Outer Segments

The Renewal of Photoreceptor Cell Outer Segments

The Renewal of Protein in Retinal Rods and Cones

Physiological Chemistry of the Eye

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