Autotransplantation of Epithelial Cells in the Pig Via an Aerosol Vehicle

Fraulin, Frankie Og; Bahoric, A.; Harrop, A. Robertson; Hiruki, Tadaaki; Clarke, Howard M.

doi:10.1097/00004630-199807000-00012

Cited by 39 publications

(27 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The attention to, and understanding of, these shortcomings have led to a progressive development of techniques of skin culture and an increased use of suspensions of single cells of keratinocytes being transplanted instead of sheet grafts. Fraulin et al [5], in 1998, described a novel technique in which they used an aerosol device to spray epithelial cells on wounds in pigs. They noted that re-epithelialisation was quicker than in unsprayed controls.…”

Section: Introductionmentioning

confidence: 99%

Transplantation of cultured human keratinocytes in single cell suspension: A comparative in vitro study of different application techniques

Fredriksson

Kratz

Huss

2008

Burns

View full text Add to dashboard Cite

Transplantation of autologous cultured keratinocytes in single cell suspension is useful in the treatment of burns. The reduced time needed for culture, and the fact that keratinocytes in suspension can be transported from the laboratory to the patient in small vials, thus reducing the costs involved and be stored (frozen) in the clinic for transplantation when the wound surfaces are ready, makes it appealing. We found few published data in the literature about actual cell survival after transplantation of keratinocytes in single cell suspension and so did a comparative in vitro study, considering commonly used application techniques. Human primary keratinocytes were transplanted in vitro in a standard manner using different techniques. Keratinocytes were counted before and after transplantation, were subsequently allowed to proliferate, and counted again on days 4, 8, and 14 by vital staining. Cell survival varied, ranging from 47% to >90%, depending on the technique. However, the proliferation assays showed that the differences in numbers diminished after 8 days of culture. Our findings indicate that a great number of cells die during transplantation but that this effect is diminished if cells are allowed to proliferate in an optimal milieu. A burned patient's wounds cannot be regarded as the optimal milieu, and using less harsh methods of transplantation may increase the take rate and wound closing properties of autologous keratinocytes transplanted in a single cell suspension.

show abstract

Section: Introductionmentioning

confidence: 99%

Transplantation of cultured human keratinocytes in single cell suspension: A comparative in vitro study of different application techniques

Fredriksson

Kratz

Huss

2008

Burns

View full text Add to dashboard Cite

show abstract

“…In addition, the cell sheets are hard to handle as they are very fragile (Harris et al (1998) and Williamson et al (1995)). Thirdly, the production of CEA is labor intensive and the process of growing the sheets of cells is expensive (Fraulin et al, 1998). Chalumeau et al (1999) estimated that the cost of this treatment was approximately US$80 000 for children and US$110 000 for adults.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Travelling Waves Associated with the Modelling of Aerosolised Skin Grafts

2006

View full text Add to dashboard Cite

A previous model developed by the authors investigates the growth patterns of keratinocyte cell colonies after they have been applied to a burn site using a spray technique. In this paper we investigate a simplified one-dimensional version of the model. This model yields travelling wave solutions and we analyse the behaviour of the travelling waves. Approximations for the rate of healing and maximum values for both the active healing and the healed cell densities are obtained.

show abstract

“…Using fibrin sealant as a biological cell carrier, some groups now have introduced spray techniques [39]. This enables the dispersion and distribution of cultured cells to a maximum surface compared to our initial traditional approach without spray systems.…”

Section: Cultured Cells and Biological Carriersmentioning

confidence: 99%

Tissue engineering of cultured skin substitutes

et al. 2005

View full text Add to dashboard Cite

Skin replacement has been a challenging task for surgeons ever since the introduction of skin grafts by Reverdin in 1871. Recently, skin grafting has evolved from the initial autograft and allograft preparations to biosynthetic and tissue-engineered living skin replacements. This has been fostered by the dramatically improved survival rates of major burns where the availability of autologous normal skin for grafting has become one of the limiting factors. The ideal properties of a temporary and a permanent skin substitute have been well defined. Tissue-engineered skin replacements: cultured autologous keratinocyte grafts, cultured allogeneic keratinocyte grafts, autologous/allogeneic composites, acellular biological matrices, and cellular matrices including such biological substances as fibrin sealant and various types of collagen, hyaluronic acid etc. have opened new horizons to deal with such massive skin loss. In extensive burns it has been shown that skin substitution with cultured grafts can be a life-saving measure where few alternatives exist. Future research will aim to create skin substitutes with cultured epidermis that under appropriate circumstances may provide a wound cover that could be just as durable and esthetically acceptable as conventional split-thickness skin grafts. Genetic manipulation may in addition enhance the performance of such cultured skin substitutes. If cell science, molecular biology, genetic engineering, material science and clinical expertise join their efforts to develop optimized cell culture techniques and synthetic or biological matrices then further technical advances might well lead to the production of almost skin like new tissue-engineered human skin products resembling natural human skin.

show abstract

Autotransplantation of Epithelial Cells in the Pig Via an Aerosol Vehicle

Cited by 39 publications

References 0 publications

Transplantation of cultured human keratinocytes in single cell suspension: A comparative in vitro study of different application techniques

Transplantation of cultured human keratinocytes in single cell suspension: A comparative in vitro study of different application techniques

Analysis of Travelling Waves Associated with the Modelling of Aerosolised Skin Grafts

Tissue engineering of cultured skin substitutes

Contact Info

Product

Resources

About