Autotuning of a TEM using minimum electron dose

Koster, Abraham J.; Ruijter, W.J. de; Bos, A. van den; Mast, K.D. van der

doi:10.1016/0304-3991(89)90018-1

Cited by 50 publications

(15 citation statements)

References 24 publications

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“…Defocus changes were measured using the beam‐tilt method (Koster et al. , 1987; Koster et al. , 1989).…”

Section: Methodsmentioning

confidence: 99%

“…Image shifts were measured by cross-correlating images acquired before and after a specimen tilt increment. Defocus changes were measured using the beam-tilt method (Koster et al , 1987;Koster et al , 1989). The beam-tilt method makes use of the fact that an image shift will occur when the illuminating beam is tilted and the microscope is not in focus.…”

Section: Methodsmentioning

confidence: 99%

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Automated high‐throughput electron tomography by pre‐calibration of image shifts

Ziese

Janssen

Murk

et al. 2002

Journal of Microscopy

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SummaryElectron tomography is a versatile method for obtaining threedimensional (3D) images with transmission electron microscopy. The technique is suitable to investigate cell organelles and tissue sections (100-500 nm thick) with 4-20 nm resolution. 3D reconstructions are obtained by processing a series of images acquired with the samples tilted over different angles. While tilting the sample, image shifts and defocus changes of several µ m can occur. The current generation of automated acquisition software detects and corrects for these changes with a procedure that incorporates switching the electron optical magnification. We developed a novel method for data collection based on the measurement of shifts prior to data acquisition, which results in a five-fold increase in speed, enabling the acquisition of 151 images in less than 20 min. The method will enhance the quality of a tilt series by minimizing the amount of required focus-change compensation by aligning the optical axis to the tilt axis of the specimen stage. The alignment is achieved by invoking an amount of image shift as deduced from the mathematical model describing the effect of specimen tilt. As examples for application in biological and materials sciences 3D reconstructions of a mitochondrion and a zeolite crystal are presented.

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“…Defocus changes were measured using the beam‐tilt method (Koster et al. , 1987; Koster et al. , 1989).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Automated high‐throughput electron tomography by pre‐calibration of image shifts

Ziese

Janssen

Murk

et al. 2002

Journal of Microscopy

Self Cite

View full text Add to dashboard Cite

show abstract

“…During the 1990s, fundamental technological developments were pursued related to more suitable instrumentation, incorporation of tools required for automation (e.g., automatic focusing [12,13]), and to novel approaches in reconstruction and application of statistical image analysis techniques. In 1992, the first automated data collection system for ET was realized and optimized [14][15][16][17].…”

Section: Electron Microscopy Of Biological Samplesmentioning

confidence: 99%

Three‐Dimensional Imaging of Biomaterials with Electron Tomography

Bárcena

Koning

Koster

2012

Handbook of Nanoscopy

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“…With equal transfer functions in the two images, any multiple may be used; the peak simply shifts outwards by the appropriate factor without change of shape; with unknown and possibly differing, but real, transfer functions, the multiple must be even. Another possible application is to the three-tilt method of determining beam misalignment described by Koster et al (1989), involving a transform plane product of the form which is real apart from a linear phase factor encoding the misalignment, but suffers from unpredictable sign changes in the real part.…”

Section: Discussionmentioning

confidence: 99%

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1994

Journal of Microscopy

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SummaryTwo modifications to the conventional procedure of crosscorrelation are described, widely used for establishing the relative alignment of the members of a set of images from which a higher resolution or more interpretable restoration is sought. Both achieve a high and sharp peak in circumstances where the conventional peak is too ill defined to be recognizable: neither involves significant additional computation time. The more general method requires rough knowledge of the imaging conditions, but a variant applicable to images with axial resolution has no such requirement. In addition, a least-squares procedure is presented for achieving an optimum compromise between many pair-wise displacement measurements, preventing the accumulation of alignment errors across a set of images.

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Autotuning of a TEM using minimum electron dose

Cited by 50 publications

References 24 publications

Automated high‐throughput electron tomography by pre‐calibration of image shifts

Automated high‐throughput electron tomography by pre‐calibration of image shifts

Three‐Dimensional Imaging of Biomaterials with Electron Tomography

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