Autoxidation of brain homogenates from various animals as measured by thiobarbituric acid assay

Pothiwong, Wimon; Laorpaksa, Areerat; Pirarat, Nopadon; Sirisawadi, Sujin; Intarapanya, Jantima; Jianmongkol, Suree

doi:10.1016/j.vascn.2007.08.004

Cited by 18 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Literatürde in vitro çalışmalardan indüksiyon ortamı olarak homojenat kullananlarda 10 mM, 5 mM [14,15] hücre kültürü kullananlarda ise 3 mM, 500 μM, 200 μM H 2 O 2 [16][17][18] konsantrasyonları ile oksidatif stresin indüklendi-ği görülmektedir Homogenatlarda oksidatif stres indük-lenmese bile otooksidasyon oluşmaktadır [19]. Oksidatif stresin indüklendiğini söyleyebilmek için otooksidasyona bırakılan gruptan anlamlı olarak daha yüksek lipid peroksidasyon oluşturulması gerekmektedir.…”

Section: Discussionunclassified

The effect of alpha lipoic acid on hydrogen peroxide-induced lipid peroxidation in rat liver homogenates

Yapar¹,

Eskiocak²

2014

Turk J Bioch

View full text Add to dashboard Cite

Alfa lipoik asidin rat karaciğer homojenatlarında hidrojen peroksit ile indüklenmiş lipid peroksidasyonuna etkisi [The effect of alpha lipoic acid on hydrogen peroxide-induced lipid peroxidation in rat liver homogenates] ABSTRACT Objective: It is known that oxidant species play a role in the pathogenesis of certain diseases such as diabetes mellitus, atherosclerosis, cataract and hepatic cirrhosis. Therefore, the use of antioxidant species for therapeutic purposes has risen up in the recent years. The aim of the study was to investigate the different concentrations of alpha lipoic acid on the induced lipid peroxidation and tissue glutathione level in rat liver homogenates. Methods: Hydrogen peroxide (15 mM) has been used for induction of lipid peroxidation at liver homogenates. The experimental setups induced by lipid peroxidation have been divided into four sub-groups. Alpha lipoic acid was added in 0, 2, 4 and 8 mM concentrations into those groups, respectively. The malondialdehyde levels which is the end product of lipid peroxidation and the levels of tissue glutathione have been determined. Results: The level of malondialdehyde in the activation groups has been found to be significantly higher than to the control group. The levels of malondialdehyde in the all alpha lipoic acid groups have been found to be significantly lower than the activation group. The level of glutathione in the activation group has been detected significantly lower when it was compared to the control group. The levels of glutathione in the all alpha lipoic acid groups have been found to be significantly higher from the activation group. When the time-dependent change in the level of glutathione was investigated it was observed that these initially decrease and then started to increase. In the groups of 4 and 8 mM, this level was even over from the starting point. Conclusion: In conclusion, our findings suggest that lipid peroxidation is induced in the experimental setups where hydrogen peroxide are applied. The reason of significantly lower malondialdehyde and higher glutathione levels in alpha lipoic acid group than activation groups may be a result of the antioxidant property of alpha lipoic acid.

show abstract

Section: Discussionunclassified

The effect of alpha lipoic acid on hydrogen peroxide-induced lipid peroxidation in rat liver homogenates

Yapar¹,

Eskiocak²

2014

Turk J Bioch

View full text Add to dashboard Cite

show abstract

“…Thiobarbituric acids have been reported to possess interesting biological and pharmacological activities [4][5][6][7] including the inhibitors of hepatitis C virus NS5B polymerase [8], against nonalcoholic fatty liver disease [9], potent anticonvulsant [10], measured auto-oxidation of brain homogenates from various animals [11], determining formaldehyde and acetaldehyde in food [12], HIV-1 integrate inhibitors [13] and decreases liquid peroxide in human plasma [14,15]. The introduction of fluorine atoms to bioactive molecules enhance and improve their pharmacological properties [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of some new fluorine substituted thiobarbituric acid derivatives as anti HIV1 and cyclin-dependent kinase 2 (CDK2) for cell tumor division: Part I

Alharbi¹,

Abdel‐Rahman²,

Asiri

2015

Eur. J. Chem.

View full text Add to dashboard Cite

New potential enzyme inhibitors, fluorine-substituted thiobarbituric acid derivatives (2, 3, 9, 8 and 12) and their fused/isolated heterocyclic nitrogen systems (5, 6, 10 and 14) have been obtained from heterocyclization of fluorinated N, Nʹ-disubstituted thiourea (1, 7 and 11) with malonic acid followed by ring closure reactions with primary nitrogen reagents. Structures of the synthesized products have been deduced from their elemental analysis and spectral data. Anti-HIV-1 and inhibition of cyclin-dependent kinase2 (CDK2) for cell tumor division for the synthesized compounds were also evaluated.

show abstract

Lycopene pretreatment improves hepatotoxicity induced by acetaminophen in C57BL/6 mice

Bandeira¹,

Silva

Rossoni

et al. 2017

Bioorganic & Medicinal Chemistry

View full text Add to dashboard Cite

Autoxidation of brain homogenates from various animals as measured by thiobarbituric acid assay

Cited by 18 publications

References 9 publications

The effect of alpha lipoic acid on hydrogen peroxide-induced lipid peroxidation in rat liver homogenates

The effect of alpha lipoic acid on hydrogen peroxide-induced lipid peroxidation in rat liver homogenates

Synthesis of some new fluorine substituted thiobarbituric acid derivatives as anti HIV1 and cyclin-dependent kinase 2 (CDK2) for cell tumor division: Part I

Lycopene pretreatment improves hepatotoxicity induced by acetaminophen in C57BL/6 mice

Contact Info

Product

Resources

About