Auxiliary Method for Clonal Identification of <i>Staphylococcus aureus</i> by Protein Band Pattern of Released Proteins on SDS‐Polyacrylamide Gel

Seki, Kazuhiko; Sakurada, Junji; Murai, Miyo; Usui, Akemi; Seong, Hee Kyong; Jitsukawa, Hiroko; Masuda, Shōgo

doi:10.1111/j.1348-0421.1995.tb02250.x

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…This method is not sufficiently discriminative for outbreak investigations, but instead provides information on population studies. Other phenotypic typing methods, such as biotyping, serotyping, polyacrylamide gel electrophoresis (PAGE) analysis of released or cell wall bound proteins, and immunoblotting, can be used as additional methods (244,261). However, these methods are not often used because of lack of appropriate reproducibility or discriminatory power.…”

Section: Molecular Typing Of Mrsamentioning

confidence: 99%

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Finland

Salmenlinna¹,

Lyytikäinen

Kotilainen

et al. 2000

European Journal of Clinical Microbiology & Infectious Dise

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Section: Molecular Typing Of Mrsamentioning

confidence: 99%

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Finland

Salmenlinna¹,

Lyytikäinen

Kotilainen

et al. 2000

European Journal of Clinical Microbiology & Infectious Dise

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“…However, the kind of exoproteins produced vary with the strains examined. SDS-PAGE is a useful taxonomical procedure (1,6,20,23). Therefore, we tried a kinetic study of SDS-PAGE exoprotein profiles of some isolates from individual neonates during the time period investigated, and also examined the types of coagulase, enterotoxin and TSST-1 of the isolates obtained from the neonates.…”

Section: Pfge-pattern Typing Of Mrsa Isolated From Neonatal Patients mentioning

confidence: 99%

“…Although traditional methods of strain typing, such as bacteriophage typing and serotyping, may be inadequate for the purpose of epidemiological analysis of nosocomial pathogens, recently newer molecular methods, particularly the analysis of chromosomal DNA restriction patterns by pulsed-field gel electrophoresis (PFGE), have come to be regarded as useful methods for investigating the source, transmission, and spread of nosocomial MRSA infection (4,7,8,12,14,21,24). Further, phenotypic analysis, SDS-polyacrylamide gel electrophoresis (SDS-PAGE), has been used successfully to identify different strains of Staphylococcus aureus (1,6,20,23).…”

mentioning

confidence: 99%

Epidemiologic Typing of Methicillin‐Resistant Staphylococcus aureus in Neonate Intensive Care Units Using Pulsed‐Field Gel Electrophoresis

Saito

Seki

Ohara

et al. 1998

Microbiology and Immunology

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To elucidate the mode of dissemination of methicillin-resistant Staphylococcus aureus (MRSA) in neonate intensive care units (NICUs), a total of 223 isolates from 3 separate hospitals were investigated between 1994 and 1996 by a DNA fingerprinting technique with pulsed-field gel electrophoresis (PFGE). Exoprotein profiles of some strains were also examined using SDS-polyacrylamide gel-electrophoresis (SDS-PAGE) and the assessment of enzyme/toxin production such as coagulase, enterotoxin and TSST-1. Judging from the strain typing data from PFGE results and the epidemiological data,2 different types of PFGE patterns (A and B) and their subtypes (A', A" and B') were identified. The A type including A' and A" (comprising approximately 95% of the isolates) was markedly dominant. Only 5% of the isolates belonged to type B and subtype B'. On the other hand, MRSA isolated from adult patients admitted to the same hospital showed many different PFGE patterns. The results strongly suggested that some strain(s) with specific PFGE pattern(s) is prevalent in NICUs.Furthermore, isolates which expressed the same PFGE pattern did not always express the same SDS-PAGE pattern. There were some isolates with different abilities to produce coagulase, enterotoxin C and toxic-shock syndrome toxin (TSST)-1, and the abilities had no relation with a particular type of PFGE pattern. Therefore, a combination of PFGE analysis and biochemical analyses of coagulase, enterotoxin C and TSST-1 may provide us with more detailed information for the epidemiological study of MRSA in NICUs.

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Occurrence of Coagulase Serotype among Staphylococcus aureus Strains Isolated from Healthy Individuals —Special Reference to Correlation with Size of Protein‐A Gene—

Seki

Sakurada

Seong

et al. 1998

Microbiology and Immunology

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One-hundred-and-nineteen strains of Staphylococcus aureus isolated from healthy individuals for 3 years between 1991 and 1993 were subjected to an investigation on the producibility of proteins including protein A, coagulase, enterotoxins and toxic-shock syndrome toxin-1. Especially, protein A was the center of our interest. Among these strains, 69, 43, 3 and 1 strains were found to have the protein-A gene containing 5, 4, 3 and 2 IgG-binding domains, respectively. On the other hand, only one strain was devoid of the protein-A gene. There were some differences in the profile of the coagulase serotype between the group with 4 IgG-binding domains and that with 5 IgG-binding domains. Differences in the profile of toxin production were also observed between the two groups.

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Auxiliary Method for Clonal Identification of Staphylococcus aureus by Protein Band Pattern of Released Proteins on SDS‐Polyacrylamide Gel

Cited by 3 publications

References 14 publications

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Finland

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Finland

Epidemiologic Typing of Methicillin‐Resistant Staphylococcus aureus in Neonate Intensive Care Units Using Pulsed‐Field Gel Electrophoresis

Occurrence of Coagulase Serotype among Staphylococcus aureus Strains Isolated from Healthy Individuals —Special Reference to Correlation with Size of Protein‐A Gene—

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