Auxin and Its Role in Plant Development

Zažímalová, Eva; Petrášek, Jan; Benková, Eva

doi:10.1007/978-3-7091-1526-8

Cited by 50 publications

(5 citation statements)

References 745 publications

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“…Auxin regulates divergent developmental processes and helps in anisotropic cell expansion in plants ( Kutschera et al, 1987 ; Zhao, 2010 ; Zazimalová et al, 2014 ). ARF8 helps in cell expansion and proliferation during plant development ( Varaud et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Key Genes Responsible for Weedy and Cultivar Growth Types in Soybean

Basnet

Roy

et al. 2022

Front. Genet.

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In cultivated plants, shoot morphology is an important factor that influences crop economic value. However, the effects of gene expression patterns on shoot morphology are not clearly understood. In this study, the molecular mechanism behind shoot morphology (including leaf, stem, and node) was analyzed using RNA sequencing to compare weedy (creeper) and cultivar (stand) growth types obtained in F7 derived from a cross of wild and cultivated soybeans. A total of 12,513 (in leaves), 14,255 (in stems), and 11,850 (in nodes) differentially expressed genes were identified among weedy and cultivar soybeans. Comparative transcriptome and expression analyses revealed 22 phytohormone-responsive genes. We found that GIBBERELLIN 2-OXIDASE 8 (GA2ox), SPINDLY (SPY), FERONIA (FER), AUXIN RESPONSE FACTOR 8 (ARF8), CYTOKININ DEHYDROGENASE-1 (CKX1), and ARABIDOPSIS HISTIDINE KINASE-3 (AHK3), which are crucial phytohormone response genes, were mainly regulated in the shoot of weedy and cultivar types. These results indicate that interactions between phytohormone signaling genes regulate shoot morphology in weedy and cultivar growth type plants. Our study provides insights that are useful for breeding and improving crops to generate high-yield soybean varieties.

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Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Key Genes Responsible for Weedy and Cultivar Growth Types in Soybean

Basnet

Roy

et al. 2022

Front. Genet.

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“…Vibration mills and ball grinders are very well suited for simultaneous homogenization of small amounts of plant tissues using cold extraction media and plastic microvials (Barkawi et al, 2008). Many different combinations of organic solvents and mixtures of buffers have been used as extraction media for IAA metabolites (e.g., methanol/water, isopropanol/acetic acid, and isopropanol/imidazole buffer, pH 7; reviewed by Novák et al, 2014). However, aqueous sodium phosphate buffer at neutral pH is an attractive alternative to organic solvents, reducing the levels of interfering nonpolar substances (Sundberg, 1990).…”

Section: Background Informationmentioning

confidence: 99%

Quantitative Auxin Metabolite Profiling Using Stable Isotope Dilution UHPLC‐MS/MS

et al. 2016

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Auxin, a plant hormone, is able to stimulate growth and to control developmental processes. To get a deeper level of understanding of how auxin activity is regulated in planta, we have developed a sensitive and selective mass spectrometry‐based protocol for multiplex quantification of indole 3‐acetic acid (IAA) as well as its precursors and degradation products. As part of the protocol development, we have also established a derivatization protocol for the IAA precursors IPyA and IAAld, compounds that are highly labile during sample extraction and purification. This article describes a standard isotope dilution method for quantitative profiling of the IAA metabolome in small amounts of plant tissues. © 2016 by John Wiley & Sons, Inc.

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“…Above integration is achieved by the transfer of information between different organs using plant hormones, small metabolites, and peptides. Among the plant hormones, auxin [Indole acetic acid (IAA)] plays a major role enabling short-term responses to environment such geotropism and phototropism, of long-term adaptation to environment, such as onset of dormancy or determination of plant architecture (Zažímalová et al 2014;Vanneste and Friml 2009;Davies 2004)). Besides being a master regulator of a plethora of plant developmental responses, auxin also plays an important role in modulating plant growth responses to different abiotic or biotic stresses (Kazan and Manners 2009;Cheyong et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Polar Auxin Transport in Tomato Polycotyledon Mutant Seems to be Related to Glutathione Levels

Nongmaithem

Ponukumatla

Sreelakshmi

et al. 2020

J Plant Growth Regul

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Glutathione-dependent root growth in Arabidopsis is linked to polar auxin transport (PAT). Arabidopsis mutants with reduced glutathione (GSH) levels also show reduced PAT.To gain an insight into the relationship between PAT and GSH level, we analysed tomato polycotyledon mutant, pct1-2, which has enhanced PAT. Microarray analysis of gene expression in pct1-2 mutant revealed underexpression of several genes related to glutamate and glutathione metabolism. In consonance with microarray analysis, enzymatic as well as in-vivo assay revealed higher glutathione level in the early phase of pct1-2 seedling growth than WT.The inhibition of auxin transport by 2,3,5-triiodobenzoic acid (TIBA) reduced both GSH level and PIN1 expression in pct1-2 root tips. The reduction of in vivo GSH accumulation in pct1-2 root tips by buthionine sulfoximine (BSO) stimulated elongation of the short root of pct1-2 mutant akin to TIBA. The rescue of short root phenotype of pct1-2 mutant was restricted to TIBA and BSO. The other auxin transport inhibitors 1-N-naphthylphthalamic acid (NPA), 2-[4-(diethylamino)-2-hydroxybenzoyl] benzoic acid (BUM), 3-chloro-4-hydroxyphenylacetic acid (CHPAA), brefeldin and gravacin inhibited root elongation in both WT and pct1-2 mutant.Our results indicate a relationship between PAT and GSH level in tomato akin to Arabidopsis.Our work also highlights that TIBA rescues short root phenotype of the pct1-2 mutant by acting on a PAT component distinct from the site of action of other PAT inhibitors.

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Auxin and Its Role in Plant Development

Cited by 50 publications

References 745 publications

Identification and Characterization of Key Genes Responsible for Weedy and Cultivar Growth Types in Soybean

Identification and Characterization of Key Genes Responsible for Weedy and Cultivar Growth Types in Soybean

Quantitative Auxin Metabolite Profiling Using Stable Isotope Dilution UHPLC‐MS/MS

Enhanced Polar Auxin Transport in Tomato Polycotyledon Mutant Seems to be Related to Glutathione Levels

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