Auxin‐induced reactive oxygen species production requires the activation of phosphatidylinositol 3‐kinase

Joo, Jae Hong; Yoo, Ho Jung; Hwang, Inhwan; Lee, Jae-Sung; Nam, Kee‐Hyun; Bae, Yun Soo

doi:10.1016/j.febslet.2005.01.018

Cited by 130 publications

(105 citation statements)

References 37 publications

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“…To achieve this, pollen tubes undergo highly polarized, tip-localized expansion, which depends on a complex signaling network that includes ion fluxes and gradients, cytoskeletal reorganization, and membrane trafficking (Hepler et al, 2001). Among the components of the signaling network for tip growth, reactive oxygen species, Ca 21 , cytoskeleton, or membrane trafficking have been shown to be affected by PI3K in other plant cell types, including guard cells (Jung et al, 2002;Park et al, 2003;Joo et al, 2005;Leshem et al, 2007;Choi et al, 2008). In particular, it is worth mentioning that a major G-actin-binding protein present in pollen, profilin, was recently shown to bind VPS34/PI3K from bean (Phaseolus vulgaris; Aparicio-Fabre et al, 2006) and that PI3K activity in cultured carrot (Daucus carota) cells associates with the cytoskeleton (Dove et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…PtdIns(3)P is essential for normal plant growth (Welters et al, 1994) and has been implicated in diverse physiological functions, including root nodule formation (Hong and Verma, 1994), auxin-induced production of reactive oxygen species and root gravitropism (Joo et al, 2005), root hair curling and Rhizobium infection in Medicago truncatula (Peleg-Grossman et al, 2007), increased plasma membrane endocytosis and the intracellular production of reactive oxygen species in salt tolerance response (Leshem et al, 2007), stomatal closing movement (Jung et al, 2002;Park et al, 2003), and root hair elongation . Cellular and biochemical mechanisms of action of PtdIns(3)P are the focus of intense investigation.…”

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confidence: 99%

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The Arabidopsis Phosphatidylinositol 3-Kinase Is Important for Pollen Development

et al. 2008

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Phosphatidylinositol 3-kinase has been reported to be important for normal plant growth. To characterize the role of the enzyme further, we attempted to isolate Arabidopsis (Arabidopsis thaliana) plants that do not express the gene, but we could not recover homozygous mutant plants. The progeny of VPS34/vps34 heterozygous plants, harboring a T-DNA insertion, showed a segregation ratio of 1:1:0 for wild-type, heterozygous, and homozygous mutant plants, indicating a gametophytic defect. Genetic transmission analysis showed that the abnormal segregation ratio was due to failure to transmit the mutant allele through the male gametophyte. Microscopic observation revealed that 2-fold higher proportions of pollen grains in heterozygous plants than wild-type plants were dead or showed reduced numbers of nuclei. Many mature pollen grains from the heterozygous plants contained large vacuoles even until the mature pollen stage, whereas pollen from wild-type plants contained many small vacuoles beginning from the vacuolated pollen stage, which indicated that vacuoles in many of the heterozygous mutant pollen did not undergo normal fission after the first mitotic division. Taken together, our results suggest that phosphatidylinositol 3-kinase is essential for vacuole reorganization and nuclear division during pollen development.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

The Arabidopsis Phosphatidylinositol 3-Kinase Is Important for Pollen Development

et al. 2008

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“…Many heavy metals, including Pb, are known to induce over production of ROS and consequently enhance lipid peroxidation, decrease the saturated fatty acids and increase the unsaturated fatty acid contents of membranes of several plant species (Halliwell and Gutteridge, 1999;Malecka et al, 2001). IAA also generates ROS, excites oxygen intermediates (De Melo et al, 1992;Joo et al, 2005) and increases MDA content in various rat tissues (Celik and Tuluce, 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of indole-3-acetic acid on lead accumulation in maize (Zea mays L.) seedlings and the relevant antioxidant response

Wang

Shan

Wen

et al. 2007

Environmental and Experimental Botany

107

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The effect of indole-3-acetic acid (IAA) on lead (Pb) accumulation in maize (Zea mays L. cv. TY2) seedlings, and the responses of maize to Pb, IAA or Pb + IAA stress were investigated under hydroponic conditions. Exogenous IAA significantly increased Pb accumulation in roots, but significantly decreased Pb accumulation in shoots over the culture period of 5 days. Increased Pb uptake in maize roots partly took place through calcium and potassium channels, and also depended upon membrane potential. Lipid peroxidation in terms of malondialdehyde (MDA) content was enhanced, indicating that Pb and/or IAA caused oxidative stress in maize seedlings. Under stress conditions the antioxidant defense mechanism was activated. Some antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were up-regulated compared to the control. Pb, IAA or Pb + IAA treatments resulted in a general reduction of macro-and micronutrient contents, especially of K, Ca and Mn. Compared to a single Pb or IAA treatment, treatment with a combination of Pb + IAA induced more severe oxidative stress on maize roots and shoots as inferred from the higher observed MDA and lower nutrient contents. A Fourier transform infrared (FTIR) spectroscopic study indicated that Pb reacted with the carboxylic acid group of the IAA pyrrole cycle.

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“…It is synthesized by phosphatidylinositol 3-kinase (PI3K), which in turn is encoded by the only PI3K gene reported in plants, VPS34. PtdIns(3)P and PI3K are essential for normal plant growth (Welters et al, 1994) and have been implicated in diverse physiological functions, including root nodule formation (Hong and Verma, 1994), auxin-induced production of ROS and root gravitropism (Joo et al, 2005), root hair curling and Rhizobium infection in Medicago truncatula (PelegGrossman et al, 2007), increased plasma membrane endocytosis and the intracellular production of ROS in the salt tolerance response (Leshem et al, 2007), and stomatal closing movement (Jung et al, 2002;Park et al, 2003).…”

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confidence: 99%

“…We hypothesized that PtdIns(3)P is important for tip growth of root hair cells because (1) ROS and the cytoskeleton, essential factors for root hair cell growth, are modulated by PtdIns(3)P in other cell types (Park et al, 2003;Joo et al, 2005;Leshem et al, 2007;Choi et al, 2008) and (2) PtdIns(3)P is essential for normal morphology and movement of endosomes in root hairs (Voigt et al, 2005). Using pharmacological and genetic tools, we found that the maintenance of a normal level of PtdIns(3)P is required for root hair elongation.…”

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confidence: 99%

Roles of Phosphatidylinositol 3-Kinase in Root Hair Growth

et al. 2008

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The root hair is a model system for understanding plant cell tip growth. As phosphatidylinositol 3-phosphate [PtdIns(3)P] has been shown in other plant cell types to regulate factors that affect root hair growth, including reactive oxygen species (ROS) levels, cytoskeleton, and endosomal movement, we hypothesized that PtdIns(3)P is also important for root hair elongation. The enzyme that generates PtdIns(3)P, phosphatidylinositol 3-kinase (PI3K), was expressed in root hair cells of transgenic plants containing the PI3K promoter:b-glucuronidase reporter construct. To obtain genetic evidence for the role of PtdIns(3)P in root hair elongation, we attempted to isolate Arabidopsis (Arabidopsis thaliana) mutant plants that did not express the gene VPS34 encoding the PI3K enzyme. However, the homozygous mutant was lethal due to gametophytic defects, and heterozygous plants were not discernibly different from wild-type plants. Alternatively, we made transgenic plants expressing the PtdIns(3)P-binding FYVE domain in the root hair cell to block signal transduction downstream of PtdIns(3)P. These transgenic plants had shorter root hairs and a reduced hair growth rate compared with wild-type plants. In addition, LY294002, a PI3K-specific inhibitor, inhibited root hair elongation but not initiation. In LY294002-treated root hair cells, endocytosis at the stage of final fusion of the late endosomes to the tonoplast was inhibited and ROS level decreased in a dose-dependent manner. Surprisingly, the LY294002 effects on ROS and root hair elongation were similar in rhd2 mutant plants, suggesting that RHD2 was not the major ROS generator in the PtdIns(3)P-mediated root hair elongation process. Collectively, these results suggest that PtdIns(3)P is required for maintenance of the processes essential for root hair cell elongation.

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Auxin‐induced reactive oxygen species production requires the activation of phosphatidylinositol 3‐kinase

Cited by 130 publications

References 37 publications

The Arabidopsis Phosphatidylinositol 3-Kinase Is Important for Pollen Development

The Arabidopsis Phosphatidylinositol 3-Kinase Is Important for Pollen Development

Effect of indole-3-acetic acid on lead accumulation in maize (Zea mays L.) seedlings and the relevant antioxidant response

Roles of Phosphatidylinositol 3-Kinase in Root Hair Growth

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