Auxins differentially regulate root system architecture and cell cycle protein levels in maize seedlings

Cruz, Enrique Martínez-de la; García-Ramírez, Elpidio; Vázquez‐Ramos, Jorge M.; Cruz, Homero Reyes de la; López‐Bucio, José

doi:10.1016/j.jplph.2014.11.012

Cited by 32 publications

(22 citation statements)

References 64 publications

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“…Auxin specifically binds and triggers the degradation of the SKP2A, which is important for the stability of the cell division transcription factors E2FC/DBP demonstrating that auxin directly modulates cell-cycle progression (Jurado et al, 2010). In addition, auxin regulates transcription of cellcycle control genes (Himanen et al, 2002;Jansen et al, 2012;Martínez-de la Cruz et al, 2015;Roudier et al, 2003), and the transcription of auxin-responsive genes fluctuates during cell cycle progression (Breyne et al, 2002;Menges et al, 2005). Therefore, it is likely that auxin accumulation and signaling are dynamic during cell cycle progression.…”

Section: Discussionmentioning

confidence: 99%

A DII Domain-Based Auxin Reporter Uncovers Low Auxin Signaling during Telophase and Early G1

Mir¹,

Aranda²,

Biaocchi

et al. 2016

Plant Physiol.

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A sensitive and dynamically responsive auxin signaling reporter based on the DII domain of the INDOLE-3-ACETIC ACID28 (IAA28, DII) protein from Arabidopsis (Arabidopsis thaliana) was modified for use in maize (Zea mays). The DII domain was fused to a yellow fluorescent protein and a nuclear localization sequence to simplify quantitative nuclear fluorescence signal. DII degradation dynamics provide an estimate of input signal into the auxin signaling pathway that is influenced by both auxin accumulation and F-box coreceptor concentration. In maize, the DII-based marker responded rapidly and in a dose-dependent manner to exogenous auxin via proteasome-mediated degradation. Low levels of DII-specific fluorescence corresponding to high endogenous auxin signaling occurred near vasculature tissue and the outer layer and glume primordia of spikelet pair meristems and floral meristems, respectively. In addition, high DII levels were observed in cells during telophase and early G1, suggesting that low auxin signaling at these stages may be important for cell cycle progression.

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Section: Discussionmentioning

confidence: 99%

A DII Domain-Based Auxin Reporter Uncovers Low Auxin Signaling during Telophase and Early G1

Mir¹,

Aranda²,

Biaocchi

et al. 2016

Plant Physiol.

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“…Most dicotyledons have tap root systems, while monocotyledons have fibrous root systems. The tap root system is composed of a developed primary root, lateral roots and adventitious roots, while the fibrous root system is mainly composed of adventitious roots (Martinez-de la Cruz et al, 2015;Zhang X. et al, 2019). The development of primary roots begins from embryonic development, whereas the lateral roots are initiated from asymmetrical divisions of the pericycle founder cell of primary roots.…”

Section: Introductionmentioning

confidence: 99%

Integration of Jasmonic Acid and Ethylene Into Auxin Signaling in Root Development

Zhao

Cai

et al. 2020

Front. Plant Sci.

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As sessile organisms, plants must be highly adaptable to the changing environment by modifying their growth and development. Plants rely on their underground part, the root system, to absorb water and nutrients and to anchor to the ground. The root is a highly dynamic organ of indeterminate growth with new tissues produced by root stem cells. Plants have evolved unique molecular mechanisms to fine-tune root developmental processes, during which phytohormones play vital roles. These hormones often relay environmental signals to auxin signaling that ultimately directs root development programs. Therefore, the crosstalk among hormones is critical in the root development. In this review, we will focus on the recent progresses that jasmonic acid (JA) and ethylene signaling are integrated into auxin in regulating root development of Arabidopsis thaliana and discuss the key roles of transcription factors (TFs) ethylene response factors (ERFs) and homeobox proteins in the crosstalk.

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“…The synthetic auxin NAA seems to be the most suited for an LRIS. The auxin precursor indol-3-butyric acid (IBA) might be a good alternative as it also strongly induces lateral roots and has a high bioactivity in different plants 25,26 . On the other hand, the natural auxin indole-3-acetic acid (IAA) is less stable and more easily metabolized 27 , rationalizing its weaker effect on root development in for example maize or rice 25,26 .…”

mentioning

confidence: 99%

“…The auxin precursor indol-3-butyric acid (IBA) might be a good alternative as it also strongly induces lateral roots and has a high bioactivity in different plants 25,26 . On the other hand, the natural auxin indole-3-acetic acid (IAA) is less stable and more easily metabolized 27 , rationalizing its weaker effect on root development in for example maize or rice 25,26 . The synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4D) accumulates highly in pericycle cells, partially because it is not exported from the cells 28 , impairing proper lateral root initiation and inducing artificial fused structures.…”

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confidence: 99%

Lateral Root Inducible System in <em>Arabidopsis</em> and Maize

Crombez

Roberts

Vangheluwe

et al. 2016

JoVE

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Lateral root development contributes significantly to the root system, and hence is crucial for plant growth. The study of lateral root initiation is however tedious, because it occurs only in a few cells inside the root and in an unpredictable manner. To circumvent this problem, a Lateral Root Inducible System (LRIS) has been developed. By treating seedlings consecutively with an auxin transport inhibitor and a synthetic auxin, highly controlled lateral root initiation occurs synchronously in the primary root, allowing abundant sampling of a desired developmental stage. The LRIS has first been developed for Arabidopsis thaliana, but can be applied to other plants as well. Accordingly, it has been adapted for use in maize (Zea mays). A detailed overview of the different steps of the LRIS in both plants is given. The combination of this system with comparative transcriptomics made it possible to identify functional homologs of Arabidopsis lateral root initiation genes in other species as illustrated here for the CYCLIN B1;1 (CYCB1;1) cell cycle gene in maize. Finally, the principles that need to be taken into account when an LRIS is developed for other plant species are discussed.

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Auxins differentially regulate root system architecture and cell cycle protein levels in maize seedlings

Cited by 32 publications

References 64 publications

A DII Domain-Based Auxin Reporter Uncovers Low Auxin Signaling during Telophase and Early G1

A DII Domain-Based Auxin Reporter Uncovers Low Auxin Signaling during Telophase and Early G1

Integration of Jasmonic Acid and Ethylene Into Auxin Signaling in Root Development

Lateral Root Inducible System in <em>Arabidopsis</em> and Maize

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