Avaliação da resistência a antibióticos de bactérias isoladas de efluente hospitalar

Abreu, Elenice Tavares; Pretto, João Adelmo; Caleare, Ângelo de Oliveira; Tavares, Célia Regina Granhen; Nakamura, Celso Vataru

doi:10.4025/actascitechnol.v32i1.7453

Cited by 7 publications

(9 citation statements)

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“…Potentially pathogenic organisms (e.g. Escherichia coli, Klebsiella pneumoniae, Bacillus sp., and Pseudomonas aeruginosa) have been detected in hospital effluents from several regions of Brazil and throughout the planet (Trabulsi and Althertum 2004; Depizzol et al 2005;Ekhaise and Omavwoya 2008;Resende et al 2009;Abreu et al 2010;Slekovec et al 2012). Determining their antibiotic resistance profiles is fundamental to understand the risks these organisms represent to public health.…”

Section: Discussionmentioning

confidence: 99%

Diversity and antibiotic resistance profiles of Pseudomonads from a hospital wastewater treatment plant

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Diversity and antibiotic resistance profiles of Pseudomonads from a hospital wastewater treatment plant

et al. 2015

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“…Entretanto, pesquisas sobre o papel das ETEs na disseminação e/ ou remoção das BRAs e GRAs são raras no Brasil. A maioria dos estudos investigou esgoto hospitalar e/ou fez isolamento de um grupo específico de bactérias, além de pesquisar patógenos resistentes (ABREU et al, 2010;LOPES et al, 2016;OLIVEIRA, 2011;PICÃO et al, 2013;PRADO, 2007).…”

Section: Introductionunclassified

Detecção e quantificação de bactérias resistentes aos antibióticos ampicilina e cloranfenicol em estações de tratamento de esgoto doméstico

Machado

Leal

Coêlho

et al. 2020

Eng. Sanit. Ambient.

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RESUMO Estações de tratamento de esgotos (ETEs) estão entre as principais fontes de disseminação de bactérias resistentes a antibióticos (BRAs) e genes de resistência (GRAs) no ambiente. Este trabalho quantificou a ocorrência de bactérias resistentes aos antibióticos ampicilina e cloranfenicol no esgoto bruto (EB), no efluente tratado (ET) e no lodo de duas ETEs em escala plena por um período de nove meses. As unidades investigadas utilizavam os seguintes sistemas de tratamento: ETE-A, sistema de lodos ativados convencional; e a ETE-B, reatores anaeróbios (UASB) seguidos de filtros biológicos percoladores (FBP). Os resultados evidenciaram que a ETE-A foi mais eficiente na redução das concentrações de bactérias resistentes à ampicilina e ao cloranfenicol (cerca de 1,1 e 0,7 log10UFC.mL−1 de remoção, respectivamente), quando comparada com a ETE-B (0,5log10 UFC.mL−1 de remoção para as bactérias resistentes ao cloranfenicol e nenhuma remoção para as resistentes à ampicilina). As amostras de lodo, de ambas ETEs, apresentaram elevadas concentrações de bactérias heterotróficas totais — BHTs (4,8–7,6 log10UFC.mL−1) e de BRAs (3,0–6,3 log10UFC.mL−1). A maioria das cepas resistentes à ampicilina e ao cloranfenicol isoladas foi identificada como sendo da família Enterobacteriaceae. Algumas das espécies identificadas são bactérias potencialmente patogênicas, tais como: Klebsiella pneumoniae, Aeromonas hydrophila, Escherichia coli, Enterococcus faecium, Salmonella spp. Os resultados chamam a atenção para a disseminação de BRAs, potencialmente patogênicas, no meio ambiente a partir do efluente final (proveniente do tratamento secundário) das ETEs, independentemente do tipo de sistema adotado. Fica evidente que para reduzir significativamente a concentração das BRAs no ET, este deveria passar por tratamento adicional e desinfecção.

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“…Antibiotic-resistant bacteria already widespread in the environment as a result of the indiscriminate use of antibiotics can be detected in different environments, such as hospital sewage, domestic sewage and contaminated river water, transforming effluents into very aggressive factors for the environment (Abreu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents

et al. 2019

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Different methodologies have been developed throughout the years to identify environmental microorganisms to improve bioremediation techniques, determine susceptibility profiles of bacteria in contaminated environments, and reduce the impact of microorganisms in ecosystems. Two methods of bacterial biochemical identification are compared and the susceptibility profile of bacteria, isolated from residential and industrial wastewater, is determined. Twenty-four bacteria were retrieved from the bacteria bank of the Environmental Microbiology Laboratory at the Institute of Biology (IB) of the Universidade Federal de Pelotas, Pelotas, Brazil. Bacteria were identified by conventional biochemical tests and by the VITEK ®2 automated system. Further, the susceptibility profile to antibiotics was also determined by the automated system. Six species of bacteria (Raoutella planticola, K. pneumoniae ssp. pneumoniae , Serratia marcescens, Raoutella sp., E. cloacae and Klebsiella oxytoca) were identified by conventional biochemical tests, while three species of bacteria (K. pneumoniae ssp. pneumoniae, S. marcescens and K. oxytoca ) were identified by VITEK®2 automated system. VITEK ®2 indicated agreement in 19 (79.17%) isolates and difference in five (20.83%) isolates when compared to results from conventional biochemical tests. Further, antibiotic susceptibility profile results showed that all isolates (100%) were resistant to at least one out of the 18 antibiotics tested by VITEK®2. Thus, no multi-resistant bacteria that may be used in effluent treatment systems or in bioremediation processes have been reported. Results indicate VITEK ® 2 automated system as a potential methodology in the determination of susceptibility profile and identification of environmental bacteria.

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Avaliação da resistência a antibióticos de bactérias isoladas de efluente hospitalar

Cited by 7 publications

References 1 publication

Diversity and antibiotic resistance profiles of Pseudomonads from a hospital wastewater treatment plant

Diversity and antibiotic resistance profiles of Pseudomonads from a hospital wastewater treatment plant

Detecção e quantificação de bactérias resistentes aos antibióticos ampicilina e cloranfenicol em estações de tratamento de esgoto doméstico

Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents

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