Avaliação funcional de monócitos de bovinos naturalmente infectados pelo vírus da leucose bovina

Azedo, Milton Ricardo; Blagitz, Maiara Garcia; Souza, Fernando Nogueira de; Benesi, Fernando José; Libera, Alice Maria Melville Paiva Della

doi:10.1590/s0102-09352011000500013

Cited by 10 publications

(9 citation statements)

References 30 publications

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“…As avaliações dos ensaios de fagocitose e espraiamento foram realizadas conforme previamente descrito por Della Libera et al (2006) e Azedo et al (2011). Inicialmente, procedeu-se o isolamento dos fagócitos mononucleares por aderência em lamínula de vidro, e posteriormente os índices de fagocitose e espraiamento desta população celular foram determinados, onde utilizaram--se quatro poços por amostra, sendo dois poços para avaliação da fagocitose e dois poços para avaliação do espraiamento.…”

Section: Methodsunclassified

“…Sabe-se que a capacidade funcional de fagócitos envolvidos na resposta imune pode ser avaliada, em parte, pela competência destas células de englobar e lisar partículas ou microorganismos, pela geração de espécies reativas de oxigênio (ERO), entre elas o peróxido de hidrogênio (H 2 O 2 ), ou até mesmo, pela capacidade de aderir e emitir microvilos, manifestada pela aderência e espraiamento de fagóci-tos mononucleares em lamínula de vidro (Della Libera et al 2006, Azedo et al 2011. Neste ínterim, pode-se dizer que avaliar funcionalmente os fagócitos torna possível identiϐi-car interferências que o mecanismo mais trivial de defesa da glândula mamária -os mecanismos de defesa celularpode vir a sofrer, quer por inϐluências da dieta, de estresse, mas principalmente pelo processo inϐlamatório (IDF 2003).…”

Section: Introductionunclassified

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Viabilidade celular, fagocitose e espraiamento de fagócitos mononucleares, e liberação de peróxido de hidrogênio por leucócitos de glândulas mamárias bovinas sadias e infectadas

Bastos¹,

Blagitz

Souza

et al. 2012

Pesq. Vet. Bras.

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Pesq. Vet. Bras. 32(9) The study aimed to evaluate the cell viability, the phagocytosis and spreading rates by the mononuclear phagocytes, and hydrogen peroxide (H 2 O 2 ) release by leukocytes from healthy and infected mammary glands. Thus, 94 milk samples were divided according the results of the bacteriological analysis and the somatic cell count (SCC). No signiϐicant difference was found in cell viability, the phagocytosis and spreading rates by mononuclear phagocytes between the distinct groups. Therefore, the H 2 O 2 release by leukocytes was higher in the milk samples from healthy mammary glands compared to those infected with Streptococcus spp. or Corynebacterium spp. However, when the H 2 O 2 release by phagocytes in 1mL of milk according to SCC mL -1 of each sample was estimated, it was found that milk samples from infected, infected with Staphylococcus spp. and bacteriological negative quarters with high SCC were higher than the healthy ones. It was also observed a positive correlation among SCC and cell viability or phagocytosis and spreading rates, and a negative correlation between H 2 O 2 release and cell viability or SCC. In face of, it can be concluded that the SCC, as well as their function and the cell viability, are related to mammary gland health.

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Section: Methodsunclassified

Section: Introductionunclassified

Viabilidade celular, fagocitose e espraiamento de fagócitos mononucleares, e liberação de peróxido de hidrogênio por leucócitos de glândulas mamárias bovinas sadias e infectadas

Bastos¹,

Blagitz

Souza

et al. 2012

Pesq. Vet. Bras.

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“…O ensaio de fagocitose das células da série monócito-macró-fagos foi realizado conforme descrito por Azedo et al (2011) …”

Section: Methodsunclassified

Fagocitose intensificada de Corynebacterium pseudotuberculosis por células da série monócito-macrófago de caprinos naturalmente infectados pelo vírus da artrite encefalite

et al. 2012

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Pesq. Vet. Bras. 32(12):1225-1229, dezembro 2012 1225 RESUMO.-A Artrite Encefalite Caprina (AEC) e a Linfadenite Caseosa (LC) possuem alta incidência e transmissibilidade em pequenos ruminantes. Como ambas possuem tropismo por monócitos-macrófagos e afetam mecanismos da resposta inata do hospedeiro, acredita-se que a AEC predispõe o animal a infecções por Corynebacteruim pseudo- Caprine arthritis encephalitis (CAE) and caseous lymphadenitis (CL) have high incidence and transmissibility in small ruminants. Since both virus have tropism for macrophages and monocytes and affect the innate immune response, it is believed that CAE can predispose the animal to infection by Corynebacteruim pseudotuberculosis, the etiological agent of CL. To conϐirm this hypothesis, we evaluated phagocytosis from the monocyte-macrophage cells from 30 Saanen goats. Goats were uniformly divided in two groups according to results of agar gel immunodiffusion test for CAE virus (CAEV). Peripheral blood mononuclear cells were isolated by density gradient centrifugation and the monocyte-macrophage cells were isolated from the mononuclear cells by their adhesion properties in plaques. Afterwards, phagocytosis of C. psudotuberculosis was performed for two hours at 37°C, 5% of CO 2 , and assessed by microscopic visualization. There was no difference in the percentage of monocyte-macrophage cells that phagocytozed C. bovis between groups (P=0.41). However, when phagocytosis rates were classiϐied according to the number of C. pseudotuberculosis phagocyted, the percentage of monocyte-macrophage cells that internalized more than 12 bacteria were higher in serologically CAEV positive animals compared to the serologically negative ones (P<0.001). Furthermore, a positive and signiϐicant correlation (r = 0.488; P = 0.006) between the percentage of monocyte-macrophage cells that internalized more than 12 bacteria and the percentage of monocyte that were carrying out phagocytosis was also encountered in serologically CAEV positive goats, however the same were not observed in serologically negative ones. These results demonstrated an alteration in the intensity of C. pseudotuberculosis phagocytosis by monocytes-macrophages from goats infected by CAEV. Thus, these results indicated that goats infected with CAEV may be more susceptible to CL. Fagocitose intensiϐicada de

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“…It is understandable that different viruses have direct implications on the function of the innate and adaptive immune systems (4)(5)(6). Hereupon, several studies have investigated the effects of BLV infection on lymphocyte subpopulations (2,4,5,7,8), functions of neutrophils (4,6,9), mammary epithelial cells (10)(11)(12), and monocytes (13). However, to the best of our knowledge, the effects of BLV infection on the function of milk macrophages and their viability are only speculative.…”

Section: Introductionmentioning

confidence: 99%

Milk Macrophage Function in Bovine Leukemia Virus-Infected Dairy Cows

et al. 2021

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The implications of bovine leukemia virus (BLV) on innate and adaptive immune responses have been widely investigated; however, the effects of BLV on mammary gland immunity require further investigation. The present study investigated the viability, phagocytic capacity, and intracellular production of reactive oxygen and nitrogen species (RONS) by macrophages in milk samples from dairy cows naturally infected with BLV with or without persistent lymphocytosis (PL). No effect of BLV infection in the overall number of macrophages per milliliter and in the percentage of viable macrophages among overall milk viable cells was found. Furthermore, BLV-infected dairy cows had a higher frequency of viable milk macrophages, while healthy animals had a tendency toward a higher percentage of apoptotic milk macrophages. The percentage of milk macrophages that phagocytosed Staphylococcus aureus in seronegative animals was higher than that in BLV-infected dairy cows. No effect of BLV infection on the intracellular RONS production and the intensity of phagocytosis by milk macrophages was observed. Thus, this study provides new insights into the implications of BLV infections in the bovine mammary gland.

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Avaliação funcional de monócitos de bovinos naturalmente infectados pelo vírus da leucose bovina

Cited by 10 publications

References 30 publications

Viabilidade celular, fagocitose e espraiamento de fagócitos mononucleares, e liberação de peróxido de hidrogênio por leucócitos de glândulas mamárias bovinas sadias e infectadas

Viabilidade celular, fagocitose e espraiamento de fagócitos mononucleares, e liberação de peróxido de hidrogênio por leucócitos de glândulas mamárias bovinas sadias e infectadas

Fagocitose intensificada de Corynebacterium pseudotuberculosis por células da série monócito-macrófago de caprinos naturalmente infectados pelo vírus da artrite encefalite

Milk Macrophage Function in Bovine Leukemia Virus-Infected Dairy Cows

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