Avaliação sorológica para detecção de anticorpos anti-Leishmania em cães e gatos no bairro de Santa Rita de Cássia, Município de Barra Mansa, Estado do Rio de Janeiro

Figueiredo, Fabiano Borges; Bonna, Isabel Cristina Fábregas; Nascimento, Lílian Dias; Costa, Tatiana da; Baptista, Cibele; Pacheco, Tania Maria Valente; Amendoeira, Maria Regina Reis; Madeira, Maria de Fátima

doi:10.1590/s0037-86822009000200009

Cited by 22 publications

(19 citation statements)

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“…Moreover, a poor correlation of the Kappa coefficient was observed among the techniques of direct detection, such as cytology and immunohistochemistry, and indirect detection, such as RIFI and ELISA (VIDES et al, 2011;SOBRINHO et al, 2012). In general, ELISA showed higher sensitivity when compared to RIFI (VIDES et al, 2011;SOBRINHO et al, 2012), confirming the findings of studies performed in other regions (FIGUEIREDO et al, 2009). …”

Section: Diagnosissupporting

confidence: 52%

“…present exclusively skin changes. Moreover, studies used mainly cytology or antibody detection as diagnostic techniques, which were restricted to identify the parasite genus (SOUZA et al, 2005(SOUZA et al, , 2009FIGUEIREDO et al, 2009;SILVEIRA NETO et al, 2011). Currently, feline leishmaniasis is assessed by PCR followed by sequencing and genetic alignment, a diagnostic tool that facilitates the identification of the protozoan species.…”

Section: Clinical Signsmentioning

confidence: 99%

“…In contrast to infected dogs, cats do not present hypergammaglobulinemia, which makes it necessary to use more concentrated serum in ELISA, such as 1:40 (FIGUEIREDO et al, 2009), 1:50 (SILVEIRA NETO et al, 2011), or 1:200 (VIDES et al, 2011, SOBRINHO et al, 2012), compared to a 1:400 dilution used to diagnose the disease in dogs . Unfortunately, the use of highly concentrated sera in ELISA may increase the chance of nonspecific reactions, which would influence the reliability of the results.…”

Section: Diagnosismentioning

confidence: 99%

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Clinical and epidemiological aspects of feline leishmaniasis in Brazil

et al. 2015

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Tegumentary and visceral leishmaniasis are severe and unfortunately common parasitic diseases in Brazil. Among domestic animals, dogs are considered the main urban reservoir of the protozoan parasites, however, there is evidence that infected cats can also contribute towards the disease pool. The number of cats diagnosed with leishmaniasis has greatly increased in the last few years, highlighting the importance of thorough investigations on the role of the cat in the epidemiological cycle of the disease and in public health related issues. The main clinical manifestations of leishmaniasis suffered by cats, even when infected with Leishmania chagasi, a viscerotropic species, are skin abnormalities, which can be confounded with multiple other diseases. Indirect ELISA should be used as a screening test in epidemiological investigations for being a sensitive technique, followed by more specific laboratory tests. The standardization and validation of rapid, economical and reproducible diagnostic methods, to be employed in epidemiological surveillance, are still required. Key words: Leishmania spp., cat, epidemiology, diagnosis, zoonosis, prevalence ResumoLeishmaniose tegumentar e visceral são doenças parasitárias graves e, infelizmente, comuns no Brasil. Entre os animais domésticos, o cão é considerado o principal reservatório urbano do parasito protozoário; no entanto, há indícios de que gatos infectados também possam contribuir para essas doenças. O número de gatos com diagnóstico de leishmaniose aumentou muito nos últimos anos, destacando a importância de investigações aprofundadas sobre o papel desse hospedeiro no ciclo epidemiológico da doença e em Saúde Pública. As principais manifestações clínicas da leishmaniose felina são anormalidades na pele, o que pode ser confundida com várias outras doenças, mesmo em casos de infecção por Leishmania chagasi, uma espécie viscerotrópica. ELISA indireto poderia ser usado como teste de triagem em investigações epidemiológicas, por ser um método sensível, seguido de exames laboratoriais mais específicos. A padronização e validação de métodos de diagnóstico rápidos, econômicos e reprodutíveis a serem empregados na vigilância epidemiológica ainda são necessárias.

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Section: Diagnosissupporting

confidence: 52%

Section: Clinical Signsmentioning

confidence: 99%

Section: Diagnosismentioning

confidence: 99%

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Clinical and epidemiological aspects of feline leishmaniasis in Brazil

et al. 2015

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“…It is important to highlight that sensitivity and specificity estimates of these serological methods in cats unfortunately were rarely evaluated [4, 11]. However, ELISA and WB tests were reported to be more sensitive than IFAT [10, 13–15]. Variation in sensitivity and specificity is mainly attributable to differences among the reference population studied and sampling strategies that are used for the validation procedure [16].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic performance of ELISA, IFAT and Western blot for the detection of anti-Leishmania infantum antibodies in cats using a Bayesian analysis without a gold standard

et al. 2017

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BackgroundAnti-Leishmania antibodies are increasingly investigated in cats for epidemiological studies or for the diagnosis of clinical feline leishmaniosis. The immunofluorescent antibody test (IFAT), the enzyme-linked immunosorbent assay (ELISA) and western blot (WB) are the serological tests more frequently used. The aim of the present study was to assess diagnostic performance of IFAT, ELISA and WB to detect anti-L. infantum antibodies in feline serum samples obtained from endemic (n = 76) and non-endemic (n = 64) areas and from cats affected by feline leishmaniosis (n = 21) by a Bayesian approach without a gold standard.MethodsCut-offs were set at 80 titre for IFAT and 40 ELISA units for ELISA. WB was considered positive in presence of at least a 18 KDa band. Statistical analysis was performed through a written routine with MATLAB software in the Bayesian framework. The latent data and observations from the joint posterior were simulated in the Bayesian approach by an iterative Markov Chain Monte Carlo technique using the Gibbs sampler for estimating sensitivity and specificity of the three tests.ResultsThe median seroprevalence in the sample used for evaluating the performance of tests was estimated at 0.27 [credible interval (CI) = 0.20–0.34]. The median sensitivity of the three different methods was 0.97 (CI: 0.86–1.00), 0.75 (CI: 0.61–0.87) and 0.70 (CI: 0.56–0.83) for WB, IFAT and ELISA, respectively. Median specificity reached 0.99 (CI: 0.96–1.00) with WB, 0.97 (CI: 0.93–0.99) with IFAT and 0.98 (CI: 0.94–1.00) with ELISA. IFAT was more sensitive than ELISA (75 vs 70%) for the detection of subclinical infection while ELISA was better for diagnosing clinical leishmaniosis when compared with IFAT (98 vs 97%).ConclusionsThe overall performance of all serological techniques was good and the most accurate test for anti-Leishmania antibody detection in feline serum samples was WB.

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“…A LV é uma zoonose parasitária relatada em mais de 88 países (TOZ et al, 2009), acometendo o homem e diversas espécies de mamíferos silvestres e domésticos, provocando variadas manifestações clínicas (FIGUEIREDO et al, 2009). Ela é ocasionada por protozoários da família Trypanossomatidade, os quais, uma vez dentro do organismo dos mamíferos, apresentam tropismo pelo sistema mononuclear fagociário (NEVES, 2005).…”

Section: Introductionunclassified