Avian Influenza Virus Prevalence and Subtype Diversity in Wild Birds in Shanghai, China, 2016–2018

Tang, Linlin; Tang, Wanjun; Li, Xiaofang; Hu, Chuanxia; Wu, Di; He, Guimei

doi:10.3390/v12091031

Cited by 16 publications

(16 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hosts of all AIVs were species belonged to the order Anseriformes, with Anas platyrhynchos (mallard) and Anas poecilorhyncha (spot-billed duck) the most common. Likewise, these two species were the most common hosts found in other countries [42][43][44]. Therefore, Anas platyrhynchos and Anas poecilorhyncha may have a role as sentinel species of AIVs.…”

Section: Discussionmentioning

confidence: 93%

Genetic Characteristics of Avian Influenza Virus Isolated from Wild Birds in South Korea, 2019–2020

Kim

Lee

et al. 2021

Viruses

View full text Add to dashboard Cite

Wild aquatic birds, a natural reservoir of avian influenza viruses (AIVs), transmit AIVs to poultry farms, causing huge economic losses. Therefore, the prevalence and genetic characteristics of AIVs isolated from wild birds in South Korea from October 2019 to March 2020 were investigated and analyzed. Fresh avian fecal samples (3256) were collected by active monitoring of 11 wild bird habitats. Twenty-eight AIVs were isolated. Seven HA and eight NA subtypes were identified. All AIV hosts were Anseriformes species. The HA cleavage site of 20 representative AIVs was encoded by non-multi-basic amino acid sequences. Phylogenetic analysis of the eight segment genes of the AIVs showed that most genes clustered within the Eurasian lineage. However, the HA gene of H10 viruses and NS gene of four viruses clustered within the American lineage, indicating intercontinental reassortment of AIVs. Representative viruses likely to infect mammals were selected and evaluated for pathogenicity in mice. JB21-58 (H5N3), JB42-93 (H9N2), and JB32-81 (H11N2) were isolated from the lungs, but JB31-69 (H11N9) was not isolated from the lungs until the end of the experiment at 14 dpi. None of infected mice showed clinical sign and histopathological change in the lung. In addition, viral antigens were not detected in lungs of all mice at 14 dpi. These data suggest that LPAIVs derived from wild birds are unlikely to be transmitted to mammals. However, because LPAIVs can reportedly infect mammals, including humans, continuous surveillance and monitoring of AIVs are necessary, despite their low pathogenicity.

show abstract

Section: Discussionmentioning

confidence: 93%

Genetic Characteristics of Avian Influenza Virus Isolated from Wild Birds in South Korea, 2019–2020

Kim

Lee

et al. 2021

Viruses

View full text Add to dashboard Cite

show abstract

“…Globally, a total of 112 AIV subtypes have been identi ed in wild birds, of which 49 have also been found in domestic species (2). Multiple AIV subtypes have been detected from wild birds in East Asia (2,39,40). In our study, 11 HA subtypes and nine NA subtypes in 29 different were detected in wild birds.…”

Section: Discussionmentioning

confidence: 49%

Characterization of low pathogenicity avian influenza viruses isolated from wild birds in Mongolia from 2009 to 2018

Barkhasbaatar

Gilbert

Fine

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Since 2005, highly pathogenic avian influenza A H5N1 viruses (Goose/Guangdong/1/96-H5 lineage) have spread from Asia to Africa and Europe, infecting poultry, humans and wild birds. Subsequently, avian influenza surveillance has increased worldwide. Mongolia is a good location to study influenza viruses in wild birds within Asia because the country has very low densities of domestic poultry and supports large concentrations of migratory water birds. We conducted avian influenza surveillance in Mongolia over two time periods from 2009 to 2018, utilizing environmental fecal sampling.Methods: Fresh fecal samples were collected in areas where wild water birds, including orders Anseriformes and Charadriiformes, congregated. Hemagglutinin (HA) and neuraminidase (NA) subtypes of positive samples were identified through viral isolation or molecular assays, with pathogenicity determined by HA subtype or sequencing the HA cleavage site.Results: A total of 10,222 samples were collected and tested for avian influenza virus. Of these, 7,025 fecal samples were collected from 2009-2013, and 3,197 fecal samples were collected from 2016-2018 period. Testing revealed 175 (1.7%) positive samples for low pathogenicity influenza A, including 118 samples from 2009-2013 (1.7%) and 57 samples from 2016-2018 (1.8%). Over the period and locations of surveillance no influenza A viruses were detected in association with a wild bird mortality event. HA and NA subtyping of all positives identified eleven subtypes of HA (H1-H8; H10-H12) and nine subtypes of NA (N1-N9) in 29 different combinations. Within periods, viruses were detected more frequently during the fall season (August to October, 2.5%; 95% CI 2.1-2.9%; 146/5,750) than the early summer (April to July, 0.6%; 95% CI 0.4-0.9%; 29/4,472).Conclusion: Mongolia is an important location for wild birds and is positioned as a crossroad of multiple migratory bird flyways. Breeding birds from Mongolia and further north pass through the country to wintering areas as widespread as southern Africa, the Indian Subcontinent, Southeast Asia and Australasia. Our work demonstrates the feasibility of using an affordable environmental fecal sampling approach for avian influenza surveillance and contributes to understanding the prevalence and ecology of low pathogenicity avian influenza viruses in this important location, where birds from multiple flyways mix.

show abstract

“…Large parts of East Asia and all Southeast Asia comprise the East Asian-Australasian Flyway. This flyway supports the greatest diversity and highest number of migratory birds worldwide (Tang et al, 2020). Likewise, a large number of wild aquatic birds with a great potential to serve as carriers of AIV migrate to overwintering habitats in Asia annually (Deng et al, 2013;Nakagawa et al, 2018).…”

Section: Discussionmentioning

confidence: 96%

“…Globally, a total of 112 AIV subtypes have been identified in wild birds of which 49 have been found in domestic birds (Olson et al, 2014). Only in China, twelve subtypes of HA (H1-H12) and eight subtypes of NA (N4-N9) of 21 different combinations have been identified in wild birds (Tang et al, 2020). In our analysis, we evidenced that 21 subtypes were reported from water samples, of which eleven were detected in wild bird habitats, mainly from Asian countries.…”

Section: Discussionmentioning

confidence: 99%

Avian influenza virus in water: global subtype diversity and spatial distribution patterns

Mateus-Anzola

Martínez‐López

et al. 2021

Preprint

View full text Add to dashboard Cite

The current COVID-19 pandemic highlights the need for zoonotic infectious disease surveillance. Avian influenza virus (AIV) poses a significant threat to animal and public health due to its pandemic potential. Virus-contaminated water has been suggested as an important AIV spread mechanism among multiple species. Nevertheless, few studies have characterized the global AIV subtype diversity and distribution in environmental water. Therefore, this study aims to provide an updated descriptive and phylogenetic analysis of AIVs isolated in water samples from high risk-sites for influenza outbreaks (i.e., live bird markets, poultry farms, and wild bird habitats) on a global scale. A total of 234 hemagglutinin (HA) gene sequences of 21 subtypes were reported from nine countries between 2003 – 2020. Fourteen AIV subtypes were solely reported from Asian countries. Most of the viral sequences were obtained in China and Bangladesh with 47.44% and 23.93%, respectively. Likewise, the greatest global AIV subtype diversity was observed in China with twelve subtypes. Live bird markets represented the main sampling site for AIV detection in water samples (64.10%), mostly from poultry cage water. Nevertheless, the highest subtype diversity was observed in water samples from wild bird habitats, especially from the Izumi plain and the Dongting Lake located in Japan and China, respectively. Water from drinking poultry troughs evidenced the greatest subtype diversity in live bird markets, meanwhile, environmental water used by ducks had the highest number of different subtypes in poultry farms. The maximum-likelihood phylogenetic tree showed that some HA sequences were closely related among different poultry/wild bird-related environments from different geographic origins. Therefore, the results suggest that even though the availability of HA gene sequences in public-access databases varies greatly among countries, environmental AIV surveillance represents a useful tool to elucidate potential viral diversity in wild and domestic bird populations.

show abstract

Avian Influenza Virus Prevalence and Subtype Diversity in Wild Birds in Shanghai, China, 2016–2018

Cited by 16 publications

References 24 publications

Genetic Characteristics of Avian Influenza Virus Isolated from Wild Birds in South Korea, 2019–2020

Genetic Characteristics of Avian Influenza Virus Isolated from Wild Birds in South Korea, 2019–2020

Characterization of low pathogenicity avian influenza viruses isolated from wild birds in Mongolia from 2009 to 2018

Avian influenza virus in water: global subtype diversity and spatial distribution patterns

Contact Info

Product

Resources

About