Avian leukosis virus p27 inhibits tumor necrosis factor α expression in RAW264.7 macrophages after stimulation with lipopolysaccharide

Abstract

Avian leukosis is a widely distributed disease caused by Avian leukosis virus (ALV). ALV p27 is a capsid protein and group specific antigen, whose role in host immune response is poorly understood. To explore ALV p27, we developed a RAW264.7 macrophage cell line stably expressing p27-GFP fusion protein. The cells of this line and control cell line expressing GFP protein only were compared in production of tumor necrosis factor α (TNF-α), interleukins IL-1β, IL-6, IL-12, and in proliferative activity stimulated by the lipopolysaccharide (LPS). It was found that the ALV p27 expression markedly reduced the production of TNF-α, but did not affect the production of IL-1β, IL-6, and 12, and cell proliferative activity. These results demonstrate that ALV p27 specifically inhibits TNF-α expression in the macrophages stimulated by LPS, suggesting a possible contribution of ALV p27 to immunosuppression detected in ALV-infected hosts.

Transcriptional Profiling of Host Gene Expression in Chicken Embryo Fibroblasts Infected with Reticuloendotheliosis Virus Strain HA1101

“…Dysregulated expression of IL-6 and its receptor are implicated in the pathogenesis of many diseases, including multiple immunorepressive diseases and cancers[ 48 , 49 ]. IL-6 has been shown to be up-regulated during infections with other retroviruses[ 50 ] and avian immunosuppressive viruses[ 51 – 53 ].…”

Avian Leucosis Virus-Host Interaction: The Involvement of Host Factors in Viral Replication

“…Innate immunity provides the first defense line against the invasion of pathogenic microorganisms ( 36 ). However, ALV has been confirmed to suppress the host’s innate immune response ( 37 , 38 ). Studies on the suppression mainly focused on the effects of ALV on immune cells, such as monocyte, dendritic cells (DCs), and macrophages.…”

“…However, how does ALV-J inhibit the phosphorylation of IκB and which component (viral protein, nucleotide or something else) of ALV-J affect type I interferon expression and the activation of the NF-κB are unknown. Interestingly, in the macrophage RAW264.7 stably expressing the p27-GFP fusion protein, the expression of TNF-α, IL-1β, IL-6 and IL-12, and the proliferative activity stimulated by LPS were specifically suppressed, providing evidence for the association of P27 with ALV-induced immunosuppression ( 38 ). More effort will be required to reveal the effect of ALV-J on the innate response systematically.…”

Identifikasi Virus Avian Leukosis Sub Grup J pada Peternakan Ayam Petelur Komersial di Kabupaten Tangerang Tahun 2015

“…Permasalahan ini umum terjadi pada peternakan ayam komersial sehingga penelitian ini bertujuan untuk mengetahui status penyakit ALV-J yang ada di lapangan. (Hauptli et al, 1997;Smith et al, 1998;Payne and Venugopal, 2000;Venugopal et al, 1998) (Crittenden et al, 1987;Pang et al, 2010). Dampak lain yang perlu menjadi perhatian adalah kemungkinan terjadinya mutasi varian baru yang disebabkan karena rekombinasi diantara beberapa sub grup yang bersirkulasi di lingkungan (Bai et al 1995;Girod et al 1996).…”