Avian-Pathogenic
            <i>Escherichia coli</i>
            Strains Are Similar to Neonatal Meningitis
            <i>E. coli</i>
            Strains and Are Able To Cause Meningitis in the Rat Model of Human Disease

Tivendale, Kelly A.; Logue, Catherine M.; Kariyawasam, Subhashinie; Jordan, Dianna M.; Hussein, Hussein Awad; Li, Ganwu; Wannemuehler, Yvonne; Nolan, Lisa K.

doi:10.1128/iai.00347-10

Cited by 193 publications

(184 citation statements)

References 41 publications

(70 reference statements)

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“…This strengthens the hypothesis that chickens can act as a reservoir for ExPEC infection in humans (Adiri et al, 2003;Danzeisen et al, 2013;Ewers et al, 2007;Johnson et al, 2008c;Johnson et al, 2007;Kohler and Dobrindt, 2011;Rodriguez-Siek et al, 2005a;Tivendale et al, 2010).…”

Section: Public Health Significance Of Apecsupporting

confidence: 68%

“…Despite the difficulty in identifying the origin of human ExPEC, the genetic overlap reported globally between human ExPEC and avian E. coli suggests that APEC may be a zoonotic pathogen Mora et al, 2013;Moulin-Schouleur et al, 2007). The ability of APEC to cause disease in rats, in a study by Tivendale et al (2010), shows APEC can cause disease in mammals. A study in Denmark,…”

Section: Public Health Significance Of Apecmentioning

confidence: 99%

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Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Section: Public Health Significance Of Apecsupporting

confidence: 68%

Section: Public Health Significance Of Apecmentioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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“…Isolates of E. coli that have caused neonatal meningitis in developed countries have been extensively studied (10,(14)(15)(16)(17)(18)(19). These studies have found that some serotypes and sequence types predominate among the E. coli strains that cause meningitis.…”

mentioning

confidence: 99%

Distribution of Strain Type and Antimicrobial Susceptibility of Escherichia coli Isolates Causing Meningitis in a Large Urban Setting in Brazil

et al. 2014

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The clinical management of meningitis caused by Escherichia coli is greatly complicated when the organism becomes resistant to broad-spectrum antibiotics. We sought to characterize the antimicrobial susceptibilities, sequence types (ST), and presence of known drug resistance genes of E. coli isolates that caused meningitis between 1996 and 2011 in Salvador, Brazil. We then compared these findings to those for E. coli isolates from community-acquired urinary tract infections (UTI) that occurred during the same time period and in the same city. We found that 19% of E. coli isolates from cases of meningitis and less than 1% of isolates from UTI were resistant to third-generation cephalosporins. The sequence types of E. coli isolates from cases of meningitis included ST131, ST69, ST405, and ST62, which were also found among isolates from UTI. Additionally, among the E. coli isolates that were resistant to third-generation cephalosporins, we found genes that encode the extended-spectrum beta-lactamases CTX-M-2, CTX-M-14, and CTX-M-15. These observations demonstrate that compared to E. coli strains isolated from cases of community-acquired UTI, those isolated from cases of meningitis are more resistant to third-generation cephalosporins, even though the same sequence types are shared between the two forms of extraintestinal infections.

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“…Recent studies show marked structural similarities between APEC and human extraintestinal pathogenic E. coli (ExPEC) strains, suggesting that APEC may be a source of virulence genes for human ExPEC (10,11,12). These genomic similarities appear to translate to functional similarities, as certain APEC strains are able to cause disease in a rat model of human neonatal meningitis (26).…”

mentioning

confidence: 99%

Strong Concordance Between Transcriptomic Patterns of Spleen and Peripheral Blood Leukocytes in Response to Avian Pathogenic Escherichia coli Infection

Sandford¹,

Orr²,

Li³

et al. 2012

Avian Diseases

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Avian pathogenic Escherichia coli (APEC) causes morbidity in chickens and exhibits zoonotic potential. Understanding host transcriptional responses to infection aids the understanding of protective mechanisms and serves to inform future colibacillosis control strategies. Transcriptomes of spleen and peripheral blood leukocytes (PBLs) of the same individual birds in response to APEC infection were compared to identify common response patterns and connecting pathways. More than 100 genes in three contrasts examining pathology and infection status were significantly differentially expressed in both tissues and similarly regulated. Tissue-specific differences in catalytic activity, however, appear between birds with mild and severe pathology responses. Early expression differences, between birds with severe pathology and uninfected controls, in the mitogen-activated protein kinase pathway in PBLs precede spleen responses in the p53 and cytokine-cytokine receptor pathways. Tissue bianalysis is useful in identifying genes and pathways important to the response to APEC, whose role might otherwise be underestimated in importance. Understanding host transcriptional responses to infection aids the understanding of protective mechanisms and serves to inform future colibacillosis control strategies. Transcriptomes of spleen and peripheral blood leukocytes (PBLs) of the same individual birds in response to APEC infection were compared to identify common response patterns and connecting pathways. More than 100 genes in three contrasts examining pathology and infection status were significantly differentially expressed in both tissues and similarly regulated. Tissue-specific differences in catalytic activity, however, appear between birds with mild and severe pathology responses. Early expression differences, between birds with severe pathology and uninfected controls, in the mitogen-activated protein kinase pathway in PBLs precede spleen responses in the p53 and cytokine-cytokine receptor pathways. Tissue bianalysis is useful in identifying genes and pathways important to the response to APEC, whose role might otherwise be underestimated in importance.RESUMEN. Marcada concordancia entre los patrones transcriptómicos de bazo y leucocitos de sangre periférica durante la respuesta a la infección por Escherichia coli patógena aviar.La Escherichia coli patogénica para las aves (APEC) causa morbilidad en pollos y representa un potencial zoonótico. El conocimiento de las respuestas transcripcionales del huésped durante la infección contribuye a la comprensión de los mecanismos de protección y sirve para instrumentar futuras estrategias para el control de la colibacilosis. Se compararon los transcriptomas de bazo y de leucocitos de sangre periférica (PBL) de las mismas aves individuales durante la respuesta a la infección por E. coli patogénica para las aves para identificar los patrones de respuesta comunes y los mecanismos de conexión. Más de 100 genes en tres contrastes que examinaron la patología y el estado de infección ...

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Avian-Pathogenic Escherichia coli Strains Are Similar to Neonatal Meningitis E. coli Strains and Are Able To Cause Meningitis in the Rat Model of Human Disease

Cited by 193 publications

References 41 publications

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Distribution of Strain Type and Antimicrobial Susceptibility of Escherichia coli Isolates Causing Meningitis in a Large Urban Setting in Brazil

Strong Concordance Between Transcriptomic Patterns of Spleen and Peripheral Blood Leukocytes in Response to Avian Pathogenic Escherichia coli Infection

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