Avian Somitic Cell Chimeras Using Surrogate Eggshell Technology

Mozdziak, Paul; Hodgson, Dee; Petitte, James N.

doi:10.5713/ajas.2008.70545

Cited by 4 publications

(2 citation statements)

References 28 publications

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“…Fertile hens' eggs were incubated at 38°C in a humidified incubator to yield embryos of appropriate stages (HH 10) (Hamburger and Hamilton, 1951) according to Hamburger and Hamilton, and isolated embryos from yolk (Mozdziak et al, 2008).…”

Section: Embryosmentioning

confidence: 99%

Expression of Cdc25B mRNA in Duodenal Mucosa of Chicken

Qin¹,

Zhang²,

Bao³

et al. 2010

Asian Australas. J. Anim. Sci

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Cdc25B is a mitotic regulator that might act as a starter phosphatase to initiate the positive feedback loop at the entry into mitotic (M) phase. In the present study, distribution of Cdc25B mRNA in duodenal mucosa of the chicken was demonstrated by means of in situ hybridization histochemistry (ISHH) using sense and antisense digoxigenin (DIG)-labeled RNA probes. The results showed that there were many labeled cells distributing in the duodenal mucosa of the adult chicken. Of these labeled cells, 81.60±9.63% of Cdc25B mRNA positive cells was distributed in the basilar part and mid-portion of the intestinal gland and 36.21±8.81% in the middle and basilar portion of villi of the small intestine of the chicken, respectively. Most of these labeled cells were positive in the regions of the stem cell and proliferation. The signals of ISHH decreased from basilar to upper part in the crypt of Lieberkuhn and weakened in the inferior villi of the duodenum. Moreover, the positive signals were both in the cytoplasm and cell nucleus. However, the labeled cells were negative in both the lamina muscularis mucosae and muscular layer. The results of ISHH suggested the existence of Cdc25B mRNA and vigorous proliferation activities in the duodenal mucosa of adult chicken, replenishing the cells which had sloughed off from the superior part of the villus. Our results provide some molecular evidence for a regular pattern of avian intestinal epitheliosis and functional partition and provide an approach to further study of the locations of Cdc25B in the chicken.

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Section: Embryosmentioning

confidence: 99%

Expression of Cdc25B mRNA in Duodenal Mucosa of Chicken

Qin¹,

Zhang²,

Bao³

et al. 2010

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

show abstract

“…Fertile hens' eggs were incubated at 38°C in a humidified incubator to yield embryos of appropriate stages (HH 10) according to Hamburger and Hamilton (Hamburger and Hamilton, 1951), and isolated embryos from yolk (Mozdziak et al, 2008).…”

Section: Embryosmentioning

confidence: 99%

The Expression Patterns of Cdc25A, Cdc25B, Sox2 and Mnb in Central Nervous System in Early Chicken Embryos

Zhang¹,

Qin²,

Cao³

et al. 2009

Asian Australas. J. Anim. Sci

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The sense and antisense digoxigenin-labeled RNA probes of four genes, Cdc25A, Cdc25B, Sox2 and Mnb, were produced by using SP6 and T7 RNA polymerases, respectively, and in vitro transcription. Expression patterns of the four genes were detected by in situ hybridization in HH (Hamburger and Hamilton) stage 10 chick embryos. In general, expression patterns of the four genes were similar. mRNA of the four genes was mostly restricted to the entire CNS (central nervous system). All were confined to an identical region, neural tube, neural groove and caudal neural plate, corresponding to the notochord or spinal cord, but there was some distinction in specific region or in concentration, for example in somites. The overlap in expression at the same developmental stage in the CNS suggests that the four genes may be functional similar or related in CNS development. Expression patterns of the four genes support specific roles of these regulators in the developing CNS.

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The method of chicken whole embryo culture using the eggshell windowing, surrogate eggshell andex ovoculture system

Farzaneh

Attari

Khoshnam

et al. 2018

British Poultry Science

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1. The unique accessibility of the avian embryo have made them an ideal model for the study of development and genome editing. Chicken whole embryo culture has provided important insights into toxicity tests, gene manipulation, clarifying gene functions, cell transplantation and cell tracking. 2. A simple technique for chicken manipulation is eggshell windowing, without or with seal, the latter having demonstrated some improvement in hatching rates. 3. Likewise, a surrogate eggshell system provides an accessible model for manipulation during chicken and quail development, with a higher hatchability compared to the simple windowing method. 4. The development of the chicken ex ovo culture systems in a synthetic environment as an efficient technique for imaging and microsurgery applications has enabled the study of important events of live chicken embryos at a specific time point. 5. This short review illustrates recent applications of well-designed whole embryo culture systems as a robust model for research into numerous biological mechanism, drug discovery, gene manipulating and production of functional proteins.

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Avian Somitic Cell Chimeras Using Surrogate Eggshell Technology

Cited by 4 publications

References 28 publications

Expression of Cdc25B mRNA in Duodenal Mucosa of Chicken

Expression of Cdc25B mRNA in Duodenal Mucosa of Chicken

The Expression Patterns of Cdc25A, Cdc25B, Sox2 and Mnb in Central Nervous System in Early Chicken Embryos

The method of chicken whole embryo culture using the eggshell windowing, surrogate eggshell andex ovoculture system

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