Avoiding room temperature storage and delayed cryopreservation provide better postthaw potency in hematopoietic progenitor cell grafts

Fry, Laura; Giner, Sergio Querol; Gómez, Susana; Green, Melanie; Anderson, Sally A.; Horder, Jackie; McArdle, Stephanie E.B.; Rees, Robert C.; Madrigal, J. Alejandro

doi:10.1111/trf.12006

Cited by 39 publications

(33 citation statements)

References 29 publications

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“…The extension of the time from collection to processing was associated with a decrease of cell viability. These findings are in accord with the results from other studies [5,6,13,21,26,27]. Some authors [28] considered that temperature is a key parameter and extended …”

Section: Discussionsupporting

confidence: 95%

“…Thus, the storage temperature of UCB units from collection to cryopreservation seems to be an essential factor that influences the cell viability and contributes to maintaining the final product quality. The controversial results show that UCB units must be stored before processing at 4°C [4][5][6][7] or at room temperature [8][9][10]. Other authors consider that there are no differences reflected in the quality of transplant units depending on the storage temperature [11][12][13].…”

Section: Introductionmentioning

confidence: 89%

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Factors which can influence the quality related to cell viability of the umbilical cord blood units

Dulugiac

Horeanga²,

Torcatoru³

et al. 2014

Transfusion and Apheresis Science

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Section: Discussionsupporting

confidence: 95%

Section: Introductionmentioning

confidence: 89%

Factors which can influence the quality related to cell viability of the umbilical cord blood units

Dulugiac

Horeanga²,

Torcatoru³

et al. 2014

Transfusion and Apheresis Science

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“…Peripheral blood mononuclear cells (PBMCs) were obtained from healthy donors (LifeSouth). Cryopreserved cells were thawed according to the protocol reported by Fry et al, 11 with some modifications. Briefly, aliquots of each cryopreserved graft source were thawed rapidly in a water bath at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Ex vivo virotherapy with myxoma virus does not impair hematopoietic stem and progenitor cells

et al. 2016

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Background Relapsing disease is a major challenge after hematopoietic cell transplant for hematological malignancies. Myxoma virus (MYXV) is an oncolytic virus that can target and eliminate contaminating cancer cells from auto-transplant grafts. The aims of this study were to examine the impact of MYXV on normal hematopoietic stem and progenitor cells, and define the optimal treatment conditions for ex vivo virotherapy. Methods Bone marrow (BM) and mobilized peripheral blood stem cells (mPBSCs) from patients with hematological malignancies were treated with MYXV at various time, temperature and incubation media conditions. Treated BM cells from healthy normal donors were evaluated by flow cytometry for MYXV infection, LTC-IC assay, and CFC assay. Results MYXV initiated infection in up to 45% of antigen presenting monocytes, B cells and natural killer cells; however, these infections were uniformly aborted in > 95% of all cells. Fresh graft sources showed higher levels of MYXV infection initiation than cryopreserved specimens but all cases, less than 10% of CD34+ cells could be infected after ex vivo MYXV treatment. MYXV did not impair LTC-IC colony numbers compared to mock treatment. CFC colony types and numbers were also not impaired by MYXV treatment. MYXV incubation time, temperature or culture media did not significantly change percentage of infected cells, LTC-IC colony formation or CFC colony formation. Conclusions Human hematopoietic cells are non-permissive for MYXV. Human hematopoietic stem and progenitor cells were not infected and thus unaffected by MYXV ex vivo treatment.

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“…Storage conditions together with freeze-cycles can affect the sample stability [61, 62]. Tibes et al .…”

Section: Sample Considerationsmentioning

confidence: 99%

Global Cell Proteome Profiling, Phospho-signaling and Quantitative Proteomics for Identification of New Biomarkers in Acute Myeloid Leukemia Patients

Aasebø¹,

Forthun²,

Berven³

et al. 2015

CPB

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The identification of protein biomarkers for acute myeloid leukemia (AML) that could find applications in AML diagnosis and prognosis, treatment and the selection for bone marrow transplant requires substantial comparative analyses of the proteomes from AML patients. In the past years, several studies have suggested some biomarkers for AML diagnosis or AML classification using methods for sample preparation with low proteome coverage and low resolution mass spectrometers. However, most of the studies did not follow up, confirm or validate their candidates with more patient samples. Current proteomics methods, new high resolution and fast mass spectrometers allow the identification and quantification of several thousands of proteins obtained from few tens of μg of AML cell lysate. Enrichment methods for posttranslational modifications (PTM), such as phosphorylation, can isolate several thousands of site-specific phosphorylated peptides from AML patient samples, which subsequently can be quantified with high confidence in new mass spectrometers. While recent reports aiming to propose proteomic or phosphoproteomic biomarkers on the studied AML patient samples have taken advantage of the technological progress, the access to large cohorts of AML patients to sample from and the availability of appropriate control samples still remain challenging.

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Avoiding room temperature storage and delayed cryopreservation provide better postthaw potency in hematopoietic progenitor cell grafts

Abstract: Our data suggest that HPC products are better maintained at refrigerated temperatures before cryopreservation. Delaying cryopreservation should be minimized to avoid significant losses in cell potency.

Cited by 39 publications

References 29 publications

Factors which can influence the quality related to cell viability of the umbilical cord blood units

Factors which can influence the quality related to cell viability of the umbilical cord blood units

Ex vivo virotherapy with myxoma virus does not impair hematopoietic stem and progenitor cells

Global Cell Proteome Profiling, Phospho-signaling and Quantitative Proteomics for Identification of New Biomarkers in Acute Myeloid Leukemia Patients

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