Factors which can influence the quality related to cell viability of the umbilical cord blood units

Dulugiac, Magda; Horeanga, Ionela; Torcatoru, Andrei; Bardaş, Alexandru; Matei, Georgiana; Zarnescu, Otilia

doi:10.1016/j.transci.2014.08.019

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…Amount and type of anticoagulant are variable. [132627313238404142434445464748495051525354555657585960] A study showed that anticoagulant concentration had no impact on cell viability although cell viability reduces gradually after storage for 25–48 h and higher. [61] Pope et al .…”

Section: Type and Amount Of Anticoagulantmentioning

confidence: 99%

“…[324465] Furthermore, there are controversies about the shipping temperature of CBUs and some believe that CBUs must be transported at 15°C–25°C,[6066] and some other recommend 20°C ± 2°C to processing facility within 48 h after collection[5867] and RT. [32445968]…”

Section: Time and Temperature Of Collection And Transportmentioning

confidence: 99%

“…Several studies informed that CBUs could be stored in RT[44596870] or 4°C[1726273133394653717273] or 15°C–25°C[57] before processing. Numerous studies showed that processing should be performed within 48 h[264547606674] [Figure 2].…”

Section: Time and Temperature Before Processingmentioning

confidence: 99%

“…Several studies showed that viability decreased significantly by the time. [32485059617989] Furthermore, TNC count, CD45+ viability, and MNC count change over time and these changes differed for each storage temperature. [80]…”

Section: Time and Temperature Before Processingmentioning

confidence: 99%

“…There was no correlation between the time from collection to processing and CD34+ cell count and TNC. [59] The recovery rate of TNC was declined at 48 h at RT and at 24 h at 4°C. [75]…”

Section: Time and Temperature Before Processingmentioning

confidence: 99%

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Umbilical cord blood quality and quantity: Collection up to transplantation

Mousavi

Zarrabi

Abroun

et al. 2019

Asian J Transfus Sci

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Umbilical cord blood (UCB) is an attractive source of hematopoietic stem cells for transplantation in some blood disorders. One of the major factors that influence on transplantation fate is cord blood (CB) cell count, in addition to human leukocyte antigen similarity and CD34+ cell number. Here, we review the factors that could effect on quality and quantity of CBUs. Relevant English-language literatures were searched and retrieved from PubMed using the terms: CB, quality, collection, and transplantation. The numbers of total nucleated cells (TNCs) and CD34+ cells are good indicators of CB quality because they have been associated with engraftment; thereby, whatever the TNCs in a CB unit (CBU) are higher, more likely they led to successful engraftment. Many factors influence the quantity and quality of UCB units that collect after delivery. Some parameters are not in our hands, such as maternal and infant factors, and hence, we cannot change these. However, some other factors are in our authority, such as mode of collection, type and amount of anticoagulant, and time and temperature during collection to postthaw CBUs and freeze-and-thaw procedures. By optimizing the CB collection, we can improve the quantity and quality of UCB for storage and increase the likelihood of its use for transplantation.

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Section: Type and Amount Of Anticoagulantmentioning

confidence: 99%

Section: Time and Temperature Of Collection And Transportmentioning

confidence: 99%

Section: Time and Temperature Before Processingmentioning

confidence: 99%

Section: Time and Temperature Before Processingmentioning

confidence: 99%

“…There was no correlation between the time from collection to processing and CD34+ cell count and TNC. [59] The recovery rate of TNC was declined at 48 h at RT and at 24 h at 4°C. [75]…”

Section: Time and Temperature Before Processingmentioning

confidence: 99%

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