Axolotl pronephric duct migration requires an epidermally derived,laminin 1-containing extracellular matrix and the integrin receptorα6β1

Morris, Andrea R.; Drawbridge, Julie; Steinberg, Malcolm S.

doi:10.1242/dev.00765

Cited by 9 publications

(7 citation statements)

References 42 publications

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“…Axolotl is an interesting model system as its pronephros shares a number of features with the mouse pro/mesonephros . One of the guidance mechanisms identified in Axolotl depends on the deposition of an ectoderm-derived extracellular matrix containing laminin 1, which is recognized by the ␣6␤1 integrin receptor of nephric duct cells (Drawbridge et al, 1995;Morris et al, 2003). However, we did not observe any significant difference in the expression of laminins and integrin receptors (␣6␤1, ␣3, ␣5␤1) between wild-type and Gata3 -/-embryos (data not shown).…”

Section: Gata3mentioning

confidence: 63%

Pax2/8-regulated Gata3 expression is necessary for morphogenesis and guidance of the nephric duct in the developing kidney

et al. 2006

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DEVELOPMENT 53 RESEARCH ARTICLE INTRODUCTIONThe formation of tubular epithelia from mesenchymal cells or preexisting ducts is a common feature of organ development. In the mammalian kidney, tubulogenesis first occurs during the formation of the pronephros. This primary embryonic kidney generates a single nephric (Wolffian) duct that elongates caudally towards the cloaca (Saxen, 1987). The nephric duct subsequently forms the second embryonic kidney, the mesonephros, by induction of mesonephric tubules in the adjacent mesenchymal nephric cord. When the nephric duct reaches the metanephric mesenchyme at the level of the hindlimb, interactions between both tissues initiate metanephros development by inducing budding and invasion of the ureter from the duct into the metanephric mesenchyme. The newly formed ureter branches and induces mesenchymal-epithelial transitions in the surrounding mesenchyme, thereby initiating the first of numerous cycles of nephron formation. Later during development, the nephric duct is transformed into the male genital tract (epididymis and vas deferens) or degenerates in female embryos. Hence, unraveling the molecular mechanisms of nephric duct morphogenesis is essential for our understanding of urogenital system development.Despite the central role of the pro-and mesonephros (referred to as pro/mesonephros) for kidney and genital tract formation, very few genes have so far been found to control the development of these two structures in the mouse. Notably, the Pax2 and Pax8 genes are both necessary and sufficient for the formation of the pronephros and all subsequent kidney structures (Bouchard et al., 2002). Pax2,Pax8 double-mutant embryos fail to specify the nephric lineage, as they neither undergo the initial epithelial-mesenchymal transitions leading to nephric duct formation nor activate early nephric marker genes (Bouchard et al., 2002). Pax2 and Pax8 are closely related members of the Pax family of sequence-specific transcription factors (Chi and Epstein, 2002). Pax8 -/-embryos have a severe defect in thyroid development but form normal kidneys (Mansouri et al., 1998). By contrast, Pax2 is necessary for metanephros development (Torres et al., 1995), as it controls the survival of the ureter and late nephric duct (Ostrom et al., 2000;Porteous et al., 2000;Torres et al., 1995;Bouchard, 2004) and induces the mesenchymal-epithelial transitions leading to nephron formation (Rothenpieler and Dressler, 1993). At the molecular level, Pax2 regulates the expression of important nephrogenic molecules such as Wt1 (Dehbi et al., 1996) and Gdnf (Brophy et al., 2001). These genes are expressed in the metanephric mesenchyme, and are necessary for metanephros induction and growth (Kuure et al., 2000). These data therefore point to a role of Pax2 in late mesonephros and metanephros development. However, the observation that Pax2 Pax8-/-embryos show a more severe phenotype than single-mutant embryos underscores not only the functional redundancy among the two Pax genes (Bouchard et al., 2000;Bouchard...

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Section: Gata3mentioning

confidence: 63%

Pax2/8-regulated Gata3 expression is necessary for morphogenesis and guidance of the nephric duct in the developing kidney

et al. 2006

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“…The source and identity of signals that guide the caudal elongation of the ND remain to be identified; in the axolotl, signals from the surface ectoderm, including the extracellular matrix (ECM) protein laminin1, appear important (Morris et al, 2003). In mouse, evidence for a role of laminin1 is ambiguous (Willem et al, 2002; Wu et al, 2009).…”

Section: Development Of the Nephric Duct And Ureteric Bud: Origins Ofmentioning

confidence: 99%

Patterning a Complex Organ: Branching Morphogenesis and Nephron Segmentation in Kidney Development

2010

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Summary The two major components of the kidney, the collecting system and the nephron, have different developmental histories. The collecting system arises by the reiterated branching of a simple epithelial tube, while the nephron forms from a cloud of mesenchymal cells that coalesces into epithelial vesicles. Each develops into a morphologically complex and highly differentiated structure, and together they provide essential filtration and resorption functions. In this review we will consider their embryological origin, and the genes controlling their morphogenesis, patterning and differentiation, focusing on recent advances in several areas.

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“…Deletion of these two transcription factors results in failure at the stage of specification of intermediate mesoderm, and failure of the ND to form (Bouchard et al, 2002). Studies in the chick and amphibians suggest that epithelializaton or elongation of the NDs may be regulated by Bmp4 signals from the overlying ectoderm and laminin in extracellular matrix (Morris et al, 2003;Obara-Ishihara et al, 1999). In the present study, we find that NDs of Ret mutant embryos can extend successfully to the posterior of the embryo, but…”

Section: What Factors Contribute To Nd Morphogenesis?mentioning

confidence: 99%

Nephric duct insertion is a crucial step in urinary tract maturation that is regulated by aGata3-Raldh2-Retmolecular network in mice

et al. 2011

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SUMMARYUrinary tract development depends on a complex series of events in which the ureter moves from its initial branch point on the nephric duct (ND) to its final insertion site in the cloaca (the primitive bladder and urethra). Defects in this maturation process can result in malpositioned ureters and hydronephrosis, a common cause of renal disease in children. Here, we report that insertion of the ND into the cloaca is an unrecognized but crucial step that is required for proper positioning of the ureter and that depends on Ret signaling. Analysis of Ret mutant mice at birth reveals hydronephrosis and defective ureter maturation, abnormalities that our results suggest are caused, at least in part, by delayed insertion of the ND. We find a similar set of malformations in mutants lacking either Gata3 or Raldh2. We show that these factors act in parallel to regulate ND insertion via Ret. Morphological analysis of ND extension in wild-type embryos reveals elaborate cellular protrusions at ND tips that are not detected in Ret, Gata3 or Raldh2 mutant embryos, suggesting that these protrusions may normally be important for fusion with the cloaca. Together, our studies reveal a novel Ret-dependent event, ND insertion, that, when abnormal, can cause obstruction and hydronephrosis at birth; whether ND defects underlie similar types of urinary tract abnormalities in humans is an interesting possibility.

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Axolotl pronephric duct migration requires an epidermally derived,laminin 1-containing extracellular matrix and the integrin receptorα6β1

Cited by 9 publications

References 42 publications

Pax2/8-regulated Gata3 expression is necessary for morphogenesis and guidance of the nephric duct in the developing kidney

Pax2/8-regulated Gata3 expression is necessary for morphogenesis and guidance of the nephric duct in the developing kidney

Patterning a Complex Organ: Branching Morphogenesis and Nephron Segmentation in Kidney Development

Nephric duct insertion is a crucial step in urinary tract maturation that is regulated by aGata3-Raldh2-Retmolecular network in mice

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