Axonal dwindling in early experimental diabetes. II. A study of isolated nerve fibres

Jakobsen, Johannes

doi:10.1007/bf01220630

Cited by 86 publications

(31 citation statements)

References 19 publications

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“…This is similar to our previous experiments, as is the chosen dose of streptozotocin (3,(13)(14)(15).…”

Section: Methodssupporting

confidence: 71%

“…The axon calibre increases in controls during the experimental period, whereas it decreases in diabetic rats despite normal nerve internodal length and normal skeletal length (1,3).…”

Section: Introductionmentioning

confidence: 99%

“…Streptozotocin diabetes in rats is associated with reduced myelinated nerve fibre caliber (1)(2)(3). The axon calibre increases in controls during the experimental period, whereas it decreases in diabetic rats despite normal nerve internodal length and normal skeletal length (1,3).…”

Section: Introductionmentioning

confidence: 99%

“…In the present experiments, we have examined both components of the slow axonal transport in rats of the same colony and age, and with a similar degree of diabetes as those used in the experiment demonstrating the structural axon abnormality (1)(2)(3).…”

mentioning

confidence: 99%

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Decreased axonal transport of structural proteins in streptozotocin diabetic rats.

Jakobsen¹,

Sidenius²

1980

J. Clin. Invest.

127

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“…This is similar to our previous experiments, as is the chosen dose of streptozotocin (3,(13)(14)(15).…”

Section: Methodssupporting

confidence: 71%

“…The axon calibre increases in controls during the experimental period, whereas it decreases in diabetic rats despite normal nerve internodal length and normal skeletal length (1,3).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Decreased axonal transport of structural proteins in streptozotocin diabetic rats.

Jakobsen¹,

Sidenius²

1980

J. Clin. Invest.

127

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“…Another report deals with a study of isolated nerve fibres from the rats used in the present study [8,9]. It appears from this study that decreased nerve fibre thickness in diabetic rats can also be shown with this technique.…”

Section: Discussionmentioning

confidence: 84%

Axonal dwindling in early experimental diabetes. I. A study of cross sectioned nerves

Jakobsen

1976

Diabetologia

Self Cite

172

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Summary. A diminution of the cross sectional area of myelinated fibres in the common peroneal nerve was observed in rats four weeks after the induction of diabetes with streptozotocin. Small fibres were affected more than larger ones and the axon reduction was twice that of the myelin sheath. The fibre diminution and the decreased axon/myelin ratio may explain the slowing of nerve conduction in experimental diabetes.Key words: Streptozotocin diabetes, axons, myelin sheath, nerve degeneration, neural conduction.Peripheral nerves are involved early in juvenile diabetes. Thus Gregersen (1967) demonstrated decreased motor nerve conduction velocity within the first year after the clinical onset of the disease [1]. At this time there were no clinical symptoms of neuropathy. The structural alteration underlying this functional abnormality is still unknown.Also in experimental diabetes, whether induced by streptozotocin, aUoxan or pancreatectomy, slowing of nerve conduction occurs from the very beginning. The morphological lesion has not been identified here either.A few histological studies have reported Schwann cell abnormalities several months after the induction of diabetes [2,3]. However, a recent extensive morphometric study failed to define any pathological alterations at all in diabetic animals [4].In this report of early experimental diabetes quantitative morphological changes, mainly affecting the axon, are demonstrated. The pathological lesion observed in the present study may well explain the functional abnormalitiy of nerve conduction in diabetic animals. Materials and MethodsMale Wistar rats raised on a standard rat chow diet with water ad libitum were used for the experiments. Animals 18 to 20 weeks old weighing between 330 and 380 g were selected at random for the different groups of the experiment. Diabetes was induced in ten rats by intravenous injection of 40 to 45 mg streptozotocin per kg body weight. The animals were maintained for four weeks without insulin treatment.Ten other rats were selected as a control group (control group 1). Both groups of animals were killed four weeks later.For the purpose of imitating the weight loss of the diabetic rats another group of five normal animals was deprived of food for four days and subsequently fed on a restricted daily diet of nearly 50% of the standard diet for 24 days (control group 2). With this diet it was intended to induce a weight loss somewhat greater than that observed to occur in the group of diabetic rats, to compensate for the increase in gastrointestinal filling known to occur in diabetic rats.Body weight was determined weekly in all animals. Blood glucose and 24 h urine output were measured at ten day intervals in the diabetic rats, and at the end of the experiment in the rats of the control groups. Blood samples were taken in the morning as duplicates from non-fasting animals. Blood glucose was determined by a glucose oxidase method [5] and urine glucose by a p-toluidine method. Tissue PreparationPeripheral nerves were initially fixed by vas...

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Corneal Nerve Alterations in Diabetes Mellitus

Ishida¹,

Rao²,

Cerro³

et al. 1984

Archives of Ophthalmology

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Axonal dwindling in early experimental diabetes. II. A study of isolated nerve fibres

Cited by 86 publications

References 19 publications

Decreased axonal transport of structural proteins in streptozotocin diabetic rats.

Decreased axonal transport of structural proteins in streptozotocin diabetic rats.

Axonal dwindling in early experimental diabetes. I. A study of cross sectioned nerves

Corneal Nerve Alterations in Diabetes Mellitus

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