Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents

Rodrigues, Marcelo; Festucci-Buselli, Reginaldo Alves; Silva, Luzimar Campos da; Otoni, Wagner Campos

doi:10.1590/s1516-89132014000200001

Cited by 25 publications

(11 citation statements)

References 33 publications

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“…Fortunately, the whole A. indica genome and five transcriptomes (including stem, leaf, flower, root and fruit) have been sequenced (Krishnan et al 2011 , 2012 ), which has established a solid foundation for molecular biological research on the neem tree. Though the mechanism of azadirachtin biosynthesis is unknown, a lot of research has focused on the synthesis of azadirachtin, including chemosynthesis (Veitch et al 2007 ), hairy root culture (Srivastava and Srivastava 2013 ), callus culture (Rodrigues et al 2014 ) and cell line culture in vitro (Singh and Chaturvedi 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of the terpenoid biosynthesis pathway in Azadirachta indica and Melia azedarach by RNA-seq

Wang¹,

Chen²,

Wang³

et al. 2016

SpringerPlus

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Azadirachta indica (neem) is the only source of azadirachtin, which is known for its insecticide activity. Melia azedarach is a related species of A. indica, widely distributed in the south of China. In this study, the leaf transcriptomes of these two Meliaceae plants were sequenced. More than 40 million clean reads were generated from each library. About 80 % of A. indica reads were mapped to the neem genome, while 93 % of M. azedarach reads were mapped to its assembled transcripts and unigenes dateset. After mapping and assembly, 225,972 transcripts and 91,607 unigenes of M. azedarach were obtained and 1179 new genes of A. indica were detected. Comparative analysis of the annotated differentially expressed genes (DEG) showed that all six DEGs involved in terpenoid backbone biosynthesis were up-regulated in A. indica. Chemical analysis of the two plants revealed A. indica leaves contained 2.45 % total terpenoid and nearly 20–50 µg azadirachtin per gram, whereas azadirachtin was not detected in M. azedarach and total terpenoid content was reached 1.67 %. These results give us a better insight into the transcriptomes differences between A. indica and M. azedarach, and help us to understand the terpenoid biosynthesis pathway in vivo.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-2460-6) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Comparative analysis of the terpenoid biosynthesis pathway in Azadirachta indica and Melia azedarach by RNA-seq

Wang¹,

Chen²,

Wang³

et al. 2016

SpringerPlus

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“…Exogenous additions such as elicitors and biosynthetic pathway intermediates/precursors could be a good ground of study for improved yield making it competitive with respect to other routes of production . In one such study, A. indica calli grown in liquid woody plant medium supplemented with casein acid hydrosylate, followed by elicitation with methyl jasmonate yielded highest azadirachtin concentration of 0.2470 μg/g . This corresponded to about 57% of the content in the native plant seed used as the starting parent material for initiation of the in vitro cultures.…”

Section: Trends In Azadirachtin Production From Plant Tissue Culturementioning

confidence: 99%

“…Neem ( Azadirachta indica A. Juss , family Meliaceae) is a reserve of about 300 important potentially active compounds responsible for the several biological functions of the plant such as blood purification, anti‐inflammation, antitumor, bactericidal properties, and as insect repellent . The limonoids (tetraterpenoids) constitute one‐third of the total metabolites present . Of these the most noteworthy ingredients include azadirachtin, an insecticide (mainly Azadirachtin A).…”

Section: Introductionmentioning

confidence: 99%

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Production of biopesticide azadirachtin using plant cell and hairy root cultures

Thakore

Srivastava

2017

Engineering in Life Sciences

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The extensive use of nondegradable chemical pesticides for pest management has developed serious environmental hazards. This has necessitated the urgent need to switch over to an alternative mode of biopesticide development for mass agriculture and field crop protection. Azadirachta indica A. Juss (commonly known as neem) houses a plethora of bioactive secondary metabolites with azadirachtin being the most active constituent explored in the sector of ecofriendly and biodegradable biopesticides characterized by low toxicity toward nontarget organisms. It has been reported that the highest content of azadirachtin and related limonoids is present in the seeds, available once in a year. Moreover, the inconsistent content and purity of the metabolites in whole plant makes it imperative to tap the potential of in vitro plant tissue culture applications, which would allow for several controlled manipulations for better yield and productivities. This review gives a summarized literature of the applied research and achievements in plant cell/hairy cultures of A. indica A. Juss mainly in context with the biopesticide azadirachtin and applications thereof.

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“…The increasing importance of this species as the source of the medicinal metabolites and biopesticide terpenoids and tannins demands extensive research on growth conditions acting in processes of cell differentiation, aiming to increase the production of metabolites of high economic value. Considering that the extraction of terpenoids from Neem plants has problems of variability in content because of the genetic variability and lower quality of plant material (Kumar & Parmar 1997), biotechnological techniques can ensure the regular supply of high quality homogeneous raw material and increase the production of this highly valuable active metabolite, as research into in vitro growing conditions and plant cell culture, or even the enhancement biosynthesis in cell suspension culture using chemical elicitors, is ongoing (Rodrigues et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Leaf anatomy micromorphometry plasticity and histochemistry of Azadirachta indica during acclimatization

et al. 2020

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Environmental conditions of grow can modify leaf structure and metabolite production. Neem plants produce a high amount of medicinal metabolites and contain biopesticide terpenoids with low toxicity. However, the high genetic variation and the low material quality, besides the environmental modifications warn to the need of biotechnological techniques to ensure the production of high quality metabolites. The aim was to investigate leaf structural and histochemical characteristics of Azadirachta indica grown in vitro, in vivo and acclimatized condition. It was found anatomical differences among the environments, with higher leaf thickness associated to in vivo conditions, as well as were more evenly distributed stomata. Those modifications did not qualitatively affect the production of medicinal metabolites and biopesticides. Terpenes and tannins were observed in specialized cells called idioblasts, located in the mesophyll and in the midrib region, respectively. Thus, in a qualitative approach, we can affirm that the different environments do not modify metabolites production. Increased production of these bioactive compounds could be achieved by isolation and in vitro culture of idioblasts as a new source of research in plant biotechnology.

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Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents

Abstract: ABSTRACT

Cited by 25 publications

References 33 publications

Comparative analysis of the terpenoid biosynthesis pathway in Azadirachta indica and Melia azedarach by RNA-seq

Comparative analysis of the terpenoid biosynthesis pathway in Azadirachta indica and Melia azedarach by RNA-seq

Production of biopesticide azadirachtin using plant cell and hairy root cultures

Leaf anatomy micromorphometry plasticity and histochemistry of Azadirachta indica during acclimatization

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