Azoramide ameliorates fructose-induced nonalcoholic fatty liver disease in mice

Bağcı, Rıdvan; Şahıntürk, Varol; Şahin, Erhan

doi:10.1016/j.tice.2019.07.001

Cited by 9 publications

(5 citation statements)

References 25 publications

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“…In the H&E and Oil red O staining, microvesicular steatosis was detected in the liver sections. The histopathological results obtained from this study were also consistent with the results of our previous study (Bagci et al, 2019). Silymarin caused the regression of NAFLD in a dose‐dependent manner.…”

Section: Discussionsupporting

confidence: 93%

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Silymarin attenuated nonalcoholic fatty liver disease through the regulation of endoplasmic reticulum stress proteins GRP78 and XBP-1 in mice

et al. 2020

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Nonalcoholic fatty liver disease (NAFLD) is an important health problem. The prevalence of NAFLD is increasing, especially in the Western countries. Although there are several intracellular pathways in NAFLD, endoplasmic reticulum (ER) stress has recently gained importance. Silymarin is an important liver‐protective biological molecule. In light of this information, we investigated mice for the effect of silymarin on ER stress in the NAFLD model. In our study, the mice were randomly divided into six groups: Control, silymarin 100 and 200 mg/kg sham, fructose‐induced NAFLD, and NAFLD + silymarin groups. After the last administrations, liver and blood samples were taken and hematoxylin‐eosin, as well as Oil red O staining, were performed. As a result, the body and liver weights, lipid profile, AST, ALT, and glucose levels, along with the ER stress markers, increased in the NAFLD‐only group. Silymarin treatments reversed most of these changes. Particularly, 200 mg/kg silymarin was more effective. Practical applications According to the results, silymarin attenuated NAFLD by decreasing the ER stress proteins GRP78 and XBP‐1. Silymarin may be therapeutic in the treatment of NAFLD as well as other ER‐stress‐based diseases. Silymarin can also be taken with food for prophylactic purposes.

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Section: Discussionsupporting

confidence: 93%

“…Increased body and liver weights in NAFLD‐induced mice decreased in a dose‐dependent manner with the administration of silymarin. Our findings supported the findings of previous studies, which demonstrated that high‐fructose‐containing diets increase liver and body weights (Bagci, Sahinturk, & Sahin, 2019; Bergheim et al, 2008).…”

Section: Discussionsupporting

confidence: 92%

Silymarin attenuated nonalcoholic fatty liver disease through the regulation of endoplasmic reticulum stress proteins GRP78 and XBP-1 in mice

et al. 2020

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“…Excessive intake of dietary fats makes the liver vulnerable to ER stress, leading to hepatocyte inflammation, pro-inflammatory cytokines, and hepatocyte mitochondrial dysfunction. It has been revealed that tazoramide has a high therapeutic potential for NAFLD and related diseases via a mechanism involving reduction of ER stress [17]. In the present study, PO and FO supplementation decreased the levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) in the liver, resulting in diminished ER stress through decreases in levels of GRP78, pIRE-1α and XBP1 in HFD-fed mice.…”

Section: Discussionsupporting

confidence: 51%

Peony seed oil protects against obesity-induced non-alcoholic fatty liver disease via modulation of endoplasmic reticulum stress

Su¹,

Tao²,

Xing³

et al. 2022

Trop. J. Pharm Res

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Purpose: To investigate the effect of Peony seed oil (PO) on non-alcoholic fatty liver disease (NAFLD), and the underlying mechanism of action. Methods: A mouse model of NAFLD was established using high-fat diet (HFD) for 12 weeks. Serum levels of triacylglycerol (TG), total cholesterol (TC), glucose and free fatty acids (FFAs) were determined. Activities of aspartate aminotransferase (AST) and alanine amino transferase (ALT) were assayed in liver homogenates, while mRNA and protein expressions of GRP78, x-box binding protein 1 (XBP1) and phosphorylated inositol-requiring enzyme 1 (p-IRE1α) were assayed in liver tissue using real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting, respectively. Expression levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) were also determined by qRT-PCR. Results: HFD diet led to significant increases in body weight as well as serum TG, TC, glucose and FFA levels. However, treatment with PO significantly reversed the effect of HFD on these parameters (p < 0.05). The mRNA expression levels of TNF- α, IL-6 and IL-1β were significantly higher in HFD control group than in normal control group, but were significantly reduced after PO treatment (p < 0.05). The protein expression levels of GRP78, XBP-1 and p-IRE1α significantly increased in the liver of NAFLD group, when compared with normal control rats, but were significantly downregulated by treatment with PO (p < 0.05). Conclusion: PO attenuates HFD-induced NAFLD via inactivation of endoplasmic reticulum stress (ERS) pathway. Thus, PO has potentials for the treatment of NAFLD but further studies are required to ascertain this.

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“…Decreased expression of ER stress molecule GRP78 contributed to reducing fasting glucose and lipid pro le, and attenuating NAFLD [97][98][99].…”

Section: Discussionmentioning

confidence: 99%

Investigation of the potential mechanism of the Shugan Xiaozhi decoction for the treatment of nonalcoholic fatty liver disease based on network pharmacology and molecular docking

Xing

Yang

et al. 2022

Preprint

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Background: Nonalcoholic fatty liver disease (NAFLD) is a metabolic-related disease with its incidence increasing annually. Shugan Xiaozhi (SGXZ) decoction, a composite traditional Chinese medicinal prescription, was demonstrated to exert a therapeutic effect on NAFLD. However, the underlying mechanisms were incompletely elucidated. In this study, the potential bioactive ingredients and mechanism of the SGXZ decoction against NAFLD were explored via network pharmacology and molecular docking. Methods: Compounds in SGXZ decoction were identified and collected from literature studies, and the corresponding targets were predicted through SEA database. Potential targets related to NAFLD were overlapped by using DisGeNET and GeneCards database. The compound-target-disease and PPI network were then constructed based on the putative intersection targets of SGXZ decoction and NAFLD to recognize the key compounds and targets. Functional enrichment analysis was performed to elucidate the biological process and signaling pathway that SGXZ decoction treated NAFLD. Finally, molecular docking combined with homology modeling was applied to further verify the binding between key active compounds and targets.Results: A total of 31 active compounds and 220 corresponding targets in SGXZ decoction were collected. Moreover, 1544 overlapped targets of NAFLD were obtained, of which 78 common targets were intersected with targets of SGXZ decoction. Key compounds and targets were recognized from compound-target-disease and PPI network. Functional enrichment analysis revealed that multiple biological pathways were annotated including insulin resistance, HIF-1, PI3K-Akt, and MAPK signaling pathways. Molecular docking confirmed that gallic acid, chlorogenic acid and isochlorogenic acid A could combine firmly with all of the key targets. Conclusion: SGXZ decoction could produce a promising effect for its multi-component, multi-target and multi-biological process in the treatment of NAFLD. The present study provides the novel insight into a comprehensive understanding for further study.

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Azoramide ameliorates fructose-induced nonalcoholic fatty liver disease in mice

Cited by 9 publications

References 25 publications

Silymarin attenuated nonalcoholic fatty liver disease through the regulation of endoplasmic reticulum stress proteins GRP78 and XBP-1 in mice

Silymarin attenuated nonalcoholic fatty liver disease through the regulation of endoplasmic reticulum stress proteins GRP78 and XBP-1 in mice

Peony seed oil protects against obesity-induced non-alcoholic fatty liver disease via modulation of endoplasmic reticulum stress

Investigation of the potential mechanism of the Shugan Xiaozhi decoction for the treatment of nonalcoholic fatty liver disease based on network pharmacology and molecular docking

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