Azospirillum thiophilum sp. nov., a diazotrophic bacterium isolated from a sulfide spring

Lavrinenko, Ksenia; Chernousova, E. Yu.; Gridneva, E. V.; Дубинина, Г. А.; Акимов, В. Н.; Kuever, Jan; Lysenko, Anatoly M.; Grabovich, M. Yu.

doi:10.1099/ijs.0.018853-0

Cited by 75 publications

(24 citation statements)

References 34 publications

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“…So far, fifteen species of the Azospirillum genus have been described ([4] and references therein). However, most research efforts have been dedicated to the species Azospirillum brasilense , neglecting the potential offered by the biological diversity of this genus.…”

Section: Introductionmentioning

confidence: 99%

Genomic insights into the versatility of the plant growth-promoting bacterium Azospirillum amazonense

et al. 2011

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BackgroundThe species Azospirillum amazonense belongs to a well-known genus of plant growth-promoting bacteria. This bacterium is found in association with several crops of economic importance; however, there is a lack of information on its physiology. In this work, we present a comprehensive analysis of the genomic features of this species.ResultsGenes of A. amazonense related to nitrogen/carbon metabolism, energy production, phytohormone production, transport, quorum sensing, antibiotic resistance, chemotaxis/motility and bacteriophytochrome biosynthesis were identified. Noteworthy genes were the nitrogen fixation genes and the nitrilase gene, which could be directly implicated in plant growth promotion, and the carbon fixation genes, which had previously been poorly investigated in this genus. One important finding was that some A. amazonense genes, like the nitrogenase genes and RubisCO genes, were closer phylogenetically to Rhizobiales members than to species of its own order.ConclusionThe species A. amazonense presents a versatile repertoire of genes crucial for its plant-associated lifestyle.

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Section: Introductionmentioning

confidence: 99%

Genomic insights into the versatility of the plant growth-promoting bacterium Azospirillum amazonense

et al. 2011

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“…Besides, the genus Azospirillum also shows versatile C-and N-metabolism and better colonizing capability in the rhizosphere (Steenhoudt and Vanderleyden 2000). Until now a total of 15 species has been described in the genus Azospirillum: A. amazonense (Falk et al 1985), A. brasilense (Helsel et al 2006), A. canadense (Mehnaz et al 2007a), A. doebereinerae (Eckert et al 2001), A. halopraeferens (Reinhold et al 1987), A. irakense (Khammas et al 1989), A. largimobile (Ben Dekhil et al 1997), A. lipoferum (Tarrand et al 1978), A. melinis (Peng et al 2006), A. oryzae (Xie and Yokota 2005), A. palatum (Zhou et al 2009), A. picis (Lin et al 2009), A. rugosum (Young et al 2008), A. thiophilum (Lavrinenko et al 2010) and A. zeae (Mehnaz et al 2007b). They are distributed mainly from soils and frequently associated with grasses, cereals and crops (Kirchhof et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection and identification of the free-living nitrogen fixing genus Azospirillum by 16S rRNA-gene-targeted genus-specific primers

Lin

Shen

2011

Antonie van Leeuwenhoek

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The modern agricultural practice utilizing plant growth promoting rhizobacteria (PGPR) has brought great benefits in the promotion of crop growth. Among PGPR, Azospirillum is considered as an important genus which is not only closely-associated with plants but also shows potential in the degradation of organic contaminants. However, lack of media for selective isolation or techniques for specific detection or identification limit the exploration of these rhizobacteria. This motivated us to design a genus-specific oligonucleotide primer pair which could assist in rapid detection of species of the genus Azospirillum by means of PCR-specific amplification. The sensitivity and specificity of the newly designed primer pair Azo494-F/Azo756-R were tested against 12 Azospirillum type strains and other closely-related genera. The Azospirillum-specific 16S rRNA gene fragment (263 bp) was successfully amplified for all the reference Azospirillum species with the designed primer pair. No amplification was noted for closely-related species from other genera. The genus specificity was validated with 18 strains including environmental isolates. Interestingly, two strains assigned earlier as Azospirillum amazonense (DSM 2787(T)) and Azospirillum irakense (DSM 11586(T)) failed to produce an Azospirillum-specific fragment with this primer pair. Further phylogenetic analysis of these two isolates based on 16S rRNA gene sequences shows that these two strains might belong to other genera rather than Azospirillum. Preliminary screening of isolates and soil samples with the Azospirillum-specific primers was successful in terms of the rapid detection of Azospirillum isolates. By using real-time PCR analysis the minimum limit of Azospirillum detection was 10(2) CFU g(-1) in the seeded soil sample. The newly designed primers can be used to study the diversity of Azospirillum in ecosystems and aid in the exploration of novel species.

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“…During this process, groundwater is being enriched in H 2 S and ultimately discharged through surface springs [40,43]. Table 1 and [35,40,41,[44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59] [76], but even such low concentrations are biologically relevant and considered toxic for most metazoans [16]. Conversely, peak concentrations of H 2 S have been reported to exceed 7000 μM in some springs [79].…”

Section: Freshwater Sulphide Springs: Occurrence and Environmental Vamentioning

confidence: 99%

Patterns of Macroinvertebrate and Fish Diversity in Freshwater Sulphide Springs

Greenway

Arias‐Rodríguez²,

Diaz³

et al. 2014

Diversity

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Extreme environments are characterised by the presence of physicochemical stressors and provide unique study systems to address problems in evolutionary ecology research. Sulphide springs provide an example of extreme freshwater environments; because hydrogen sulphide's adverse physiological effects induce mortality in metazoans even at micromolar concentrations. Sulphide springs occur worldwide, but while microbial communities in sulphide springs have received broad attention, little is known about macroinvertebrates and fish inhabiting these toxic environments. We reviewed qualitative occurrence records of sulphide spring faunas on a global scale and present a quantitative case study comparing diversity patterns in sulphidic and adjacent non-sulphidic habitats across replicated river drainages in Southern Mexico. While detailed studies in most regions of the world remain scarce, available data suggests that sulphide spring faunas are characterised by low species richness. Dipterans (among macroinvertebrates) and cyprinodontiforms (among fishes) appear to dominate the communities in these habitats. At least in fish, there is evidence for the presence of highly endemic species and populations exclusively inhabiting sulphide springs. We provide a detailed discussion of traits that might predispose certain taxonomic groups to colonize sulphide springs, how colonizers subsequently adapt to cope with sulphide toxicity, and how adaptation may be linked to speciation processes. OPEN ACCESSDiversity 2014, 6 598

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Azospirillum thiophilum sp. nov., a diazotrophic bacterium isolated from a sulfide spring

Cited by 75 publications

References 34 publications

Genomic insights into the versatility of the plant growth-promoting bacterium Azospirillum amazonense

Genomic insights into the versatility of the plant growth-promoting bacterium Azospirillum amazonense

Rapid detection and identification of the free-living nitrogen fixing genus Azospirillum by 16S rRNA-gene-targeted genus-specific primers

Patterns of Macroinvertebrate and Fish Diversity in Freshwater Sulphide Springs

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