2012
DOI: 10.1007/s11274-012-1084-4
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Azotobacter vinelandii lacking the Na+-NQR activity: a potential source for producing alginates with improved properties and at high yield

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Cited by 25 publications
(12 citation statements)
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“…The GumL enzyme incorporates pyruvyl residues to the external β-mannose residues, while the acetyl residues are incorporated into internal α-mannose units by GumF, and external β-mannose by GumG (Becker et al, 1998). A high degree of pyruvylation by increasing the transcription of gumL (cloning an additional promoter upstream gumL) resulted in higher viscosity, whereas more acetyl group decreased the viscosity of the resulting EPS (Gaytán et al, 2012;Rehm, 2015). Further, researchers (Peng et al, 2012;Sponza, 2003) 512 examined the EPS extracted directly from activated sludge 513 treating various types of wastewaters.…”
Section: Polymerization and Cytoplasmic Membrane Transfermentioning
confidence: 99%
“…The GumL enzyme incorporates pyruvyl residues to the external β-mannose residues, while the acetyl residues are incorporated into internal α-mannose units by GumF, and external β-mannose by GumG (Becker et al, 1998). A high degree of pyruvylation by increasing the transcription of gumL (cloning an additional promoter upstream gumL) resulted in higher viscosity, whereas more acetyl group decreased the viscosity of the resulting EPS (Gaytán et al, 2012;Rehm, 2015). Further, researchers (Peng et al, 2012;Sponza, 2003) 512 examined the EPS extracted directly from activated sludge 513 treating various types of wastewaters.…”
Section: Polymerization and Cytoplasmic Membrane Transfermentioning
confidence: 99%
“…(Fatemeh et al, 2014), Pseudomonas, Azotobacter(Gaytan et al, 2012), Bacillus (Ashwini and Srividya, 2014), Enterobacter(Collavino et al, 2010), Burkholederia(Minaxi et al, 2013), Serratia(Dastager et al, 2011), Pantoea(Mishra et al, 2011), Rhizobium(Hungria et al, 2013), Mesorhizobium…”
mentioning
confidence: 99%
“…Implementing this strategy resulted in an alginate titer of up to 6.6 g/L with a yield of 1.9 g/g sucrose. Single gene-deletion studies in A. vinelandii (39,40) also increased alginate yield (0.66 g/g on sucrose) (Table 1), and lowered the degree of acetylation for altered molecular composition. More recently, alginate overproduction was reported during biofilm formation in a newly discovered strain Pseudomonas mandelii 6A1 (41) that was isolated from Antarctica (41).…”
Section: Alginatementioning
confidence: 96%