Antibacterial, antibiofilm, and antiquorum sensing activities of phytosynthesized silver nanoparticles fabricated fromMespilus germanicaextract against multidrug resistance ofKlebsiella pneumoniaeclinical strains

Foroohimanjili, Fatemeh; Mirzaie, Amir; Hamdi, Seyed Mohammad Mehdi; Noorbazargan, Hassan; Hedayati, Mojtaba; Dolatabadi, Aghigh; Rezaie, H; Bishak, Faezeh Mohammadpour

doi:10.1002/jobm.201900511

Cited by 29 publications

(6 citation statements)

References 38 publications

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“…Concerning its mechanism of action, Ts-AgNPs likely diffuse through the EPS layer of the biofilm, and induced ROS production, prompting bacterial death, and thereby reducing EPS layer formation and dissolution of the biofilms. The antibiofilm ability of Ts-AgNPs to diffuse through the EPS layer has been reported earlier [ 74 ].…”

Section: Discussionsupporting

confidence: 53%

Tryptone-stabilized silver nanoparticles’ potential to mitigate planktonic and biofilm growth forms of Serratia marcescens

et al. 2022

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Several microbial pathogens are capable of forming biofilms. These microbial communities pose a serious challenge to the healthcare sector as they are quite difficult to combat. Given the challenges associated with the antibiotic-based management of biofilms, the research focus has now been shifted towards finding alternate treatment strategies that can replace or complement the antibacterial properties of antibiotics. The field of nanotechnology offers several novel and revolutionary approaches to eradicate biofilm-forming microbes. In this study, we evaluated the antibacterial and antibiofilm efficacy of in-house synthesized, tryptone-stabilized silver nanoparticles (Ts-AgNPs) against the superbug Serratia marcescens . The nanoparticles were of spherical morphology with an average hydrodynamic diameter of 170 nm and considerable colloidal stability with a Zeta potential of − 24 ± 6.15 mV. Ts-AgNPs showed strong antibacterial activities with a minimum inhibitory concentration (MIC 50 ) of 2.5 µg/mL and minimum bactericidal concentration (MBC) of 12.5 µg/mL against S. marcescens . The nanoparticles altered the cell surface hydrophobicity and inhibited biofilm formation. The Ts-AgNPs were also effective in distorting pre-existing biofilms by degrading the extracellular DNA (eDNA) component of the extracellular polymeric substance (EPS) layer. Furthermore, reduction in quorum-sensing (QS)-induced virulence factors produced by S. marcescens indicated that Ts-AgNPs attenuated the QS pathway. Together, these findings suggest that Ts-AgNPs are an important anti-planktonic and antibiofilm agent that can be explored for both the prevention and treatment of infections caused by S. marcescens . Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00775-022-01977-w.

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Section: Discussionsupporting

confidence: 53%

Tryptone-stabilized silver nanoparticles’ potential to mitigate planktonic and biofilm growth forms of Serratia marcescens

et al. 2022

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“…Fourier-Transform Infrared Spectroscopy Studies. Fourier-transform infrared (FTIR) spectroscopy studies of AgNPs were made to find out the possible compounds responsible for the efficient stabilization and capping of AgNPs synthesized with M. oleifera extract [61]. The FTIR spectra of stabilized AgNPs are shown in Figure 5.…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

Antioxidant and Antibacterial Activities of Silver Nanoparticles Biosynthesized by Moringa oleifera through Response Surface Methodology

Mohammed

Mohamed

El‐Naggar

et al. 2022

Journal of Nanomaterials

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The research highlights the environmentally sustainable biosynthesis of silver nanoparticles from fresh leaves of the herbal medicinal plant Moringa oleifera. They may have been used as anti-inflammatory, anticancer, and antimicrobial agents. M. oleifera extract both reduces and stabilizes silver nanoparticles (AgNPs). Optimum factors needed for AgNP biosynthesis were studied using a central composite design (CCD) matrix. Ultraviolet-visible (UV–Vis) absorption spectroscopy, transmission electron microscopy, and Fourier-transform infrared spectroscopy were used to confirm and characterize the synthesized AgNPs. The biogenic AgNPs demonstrated substantial antibacterial potential against the pathogenic strains Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Bacillus subtilis. The antioxidant activity of biosynthesized AgNPs with M. oleifera extract increased from 11.96% when the concentration of the extract was 4 mg/mL to 63.79% at a plant concentration of 20 mg/mL. This research provides an easy and cost-effective technique for the production of stable nanoparticles, with an evaluation of their bioactivity.

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“…Further, Foroohimanjili et al investigated the antibiofilm, antibacterial, and anti quorum sensing properties of phytosynthesized silver nanoparticles (AgNPs) prepared using leaf extract of Mespilus germanica against multidrug-resistant Klebsiella pneumoniae strains. Their work revealed that at sub-MIC, AgNPs prominently inhibited the establishment of biofilm in all the biofilm-producing strains (Foroohimanjili et al, 2020).…”

Section: Silver Nanoparticlesmentioning

confidence: 99%

Phyto-fabricated Nanoparticles and Their Anti-biofilm Activity: Progress and Current Status

Das

Ghosh

Nayak

2021

Front. Nanotechnol.

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Biofilm is the self-synthesized, mucus-like extracellular polymeric matrix that acts as a key virulence factor in various pathogenic microorganisms, thereby posing a serious threat to human health. It has been estimated that around 80% of hospital-acquired infections are associated with biofilms which are found to be present on both biotic and abiotic surfaces. Antibiotics, the current mainstream treatment strategy for biofilms are often found to be futile in the eradication of these complex structures, and to date, there is no effective therapeutic strategy established against biofilm infections. In this regard, nanotechnology can provide a potential platform for the alleviation of this problem owing to its unique size-dependent properties. Accordingly, various novel strategies are being developed for the synthesis of different types of nanoparticles. Bio-nanotechnology is a division of nanotechnology which is gaining significant attention due to its ability to synthesize nanoparticles of various compositions and sizes using biotic sources. It utilizes the rich biodiversity of various biological components which are biocompatible for the synthesis of nanoparticles. Additionally, the biogenic nanoparticles are eco-friendly, cost-effective, and relatively less toxic when compared to chemically or physically synthesized alternatives. Biogenic synthesis of nanoparticles is a bottom-top methodology in which the nanoparticles are formed due to the presence of biological components (plant extract and microbial enzymes) which act as stabilizing and reducing agents. These biosynthesized nanoparticles exhibit anti-biofilm activity via various mechanisms such as ROS production, inhibiting quorum sensing, inhibiting EPS production, etc. This review will provide an insight into the application of various biogenic sources for nanoparticle synthesis. Furthermore, we have highlighted the potential of phytosynthesized nanoparticles as a promising antibiofilm agent as well as elucidated their antibacterial and antibiofilm mechanism.

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Antibacterial, antibiofilm, and antiquorum sensing activities of phytosynthesized silver nanoparticles fabricated fromMespilus germanicaextract against multidrug resistance ofKlebsiella pneumoniaeclinical strains

Cited by 29 publications

References 38 publications

Tryptone-stabilized silver nanoparticles’ potential to mitigate planktonic and biofilm growth forms of Serratia marcescens

Tryptone-stabilized silver nanoparticles’ potential to mitigate planktonic and biofilm growth forms of Serratia marcescens

Antioxidant and Antibacterial Activities of Silver Nanoparticles Biosynthesized by Moringa oleifera through Response Surface Methodology

Phyto-fabricated Nanoparticles and Their Anti-biofilm Activity: Progress and Current Status

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