B‐cell‐activating factor code and human cytomegalovirus infection in renal transplant recipients

Abstract

The objective of the present study was to explore the correlation between the BAFF signal and HCMV-TLR activation in RTx recipients complicated by HCMV. Peripheral blood (anticoagulated by EDTA-Na 2 ) and urine of 113 RTx recipients were collected; healthy volunteers were controlled. Urine HCMV-DNA was detected by real-time PCR. Recipients were classified into a positive group (>10,000 copies/mL urine) and a negative group (<10,000 copies/mL urine). ELISA results showed that sBAFF, sera anti-HCMV pp65 immunoglobulin (Ig)G antibody, and total IgG all significantly increased in recipients with positive HCMV-DNA (>10,000 copies/mL urine) (P < 0.05) compared with negative recipients (<10,000 copies/mL urine). In the positive group, HCMV-DNA copies and total IgG positively correlated with sBAFF (r ¼ 0.988 and 0.625, respectively) (P < 0.05). Luminex assay results suggested that the incidence of anti-HLA I and II and MICA antibody obviously increased in positive recipients. The expression level of BAFF and BAFF-R increased in positive recipients. A total of 88 particular genes-involved in TLR signaling pathways, NF-kB signaling pathways, and cytokinecytokine receptor signaling pathways-were detected in real-time PCR chip assay. A total of 46 genes were differentially expressed greater than two-fold, and the expression characteristic of BAFF-R was concordant with FACS results. Our findings are that activation of HCMV would induce or enhance the activation of BAFF code in RTx recipients, which may independently or cooperatively participate in renal allograft injury and decrease the long-term outcome of renal allografts.

The immunological footprint of CMV in HIV-1 patients stable on long-term ART

“…Elevated sBAFF levels have been associated with autoimmune diseases [ 27 ], graft versus host disease [ 28 ], and complications of kidney transplantation [ 29 ]. In CMV-deoxyribonucleic acid (DNA) positive renal transplant patients, sBAFF levels were higher than in CMV-DNA negative recipients, with positive correlations between CMV-DNA levels, total IgG and sBAFF [ 30 ]. Here, levels of sBAFF and total IgG correlated with antibodies reactive with CMV lysate, CMV gB and CMV IE1 in HIV patients.…”

B cell-activating factor regulates the survival of B lymphocytes infected with human cytomegalovirus

“…Our previous studies showed that BAFF signaling increased in RTx recipients who were complicated with HCMV infection, meanwhile, the level of anti-HCMV pp65-IgG and total IgG were also significantly increased [6]. Those results suggested that HCMV may induce or enhance the activation of BAFF in RTx recipients; however, there have been very few related studies since.…”