B cell activation in clinically quiescent systemic lupus erythematosus (SLE) is related to immunoglobulin levels, but not to levels of anti-dsDNA, nor to concurrent T cell activation

Spronk, PE; Gun, B. T. F. v.d.; Limburg, P. C.; Kallenberg, Cees G. M.

doi:10.1111/j.1365-2249.1993.tb06494.x

Cited by 25 publications

(10 citation statements)

References 24 publications

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“…Total white blood cell and lymphocyte count were performed on an automated Bayer ADVIA 120 blood cell counter (Bayer HealthCare, Tarrytown, NY, USA). Cell-surface expression of CD markers on leukocytes was analyzed by three-color immunofluorescence cytometry using a whole blood staining procedure as previously described ( 27 ). Briefly, 100 μL of EDTA anticoagulated whole blood was incubated with peridinin chlorophyll protein (PERC)-labeled anti-CD19 and fluorescein isothiocyanate (FITC)-labeled CD27 in combination with phycoerythrin (PE)-labeled CD20, or CD138 monoclonal antibodies (Becton Dickinson, San Jose, CA).…”

Section: Methodsmentioning

confidence: 99%

Analyses of CD27++ Plasma Cells in Peripheral Blood from Patients with Bacterial Infections and Patients with Serum Antinuclear Antibodies

et al. 2007

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The number of CD27 ++ plasma cells (PCs) in peripheral blood may be a valuable biomarker for systemic lupus erythematosus (SLE) disease management. More insights into the behavior of the PC population are, however, required to validate CD27 as a reliable biomarker. In the current study, we have monitored the PC compartment of patients with acute bacterial infections and patients with SLE and, in addition, examined the relationship between the presence of serum antinuclear antibodies (ANAs) and the number of peripheral PCs. Kinetic analyses in patients with bacterial infection revealed a 10–60-fold expansion of the CD27 ++ PC compartment that peaked at day 2–5 and returned toward normal values at day 7–9 after hospital admission. The transient expansion of the PC population appeared to be a late phenomenon in the process of recovering from a bacterial infection. SLE subjects had significantly increased frequencies of PCs compared with patients suspected of a connective tissue disease and healthy controls. In patients suspected of a connective tissue disease, no relationship was found between the presence of serum ANAs and the number of CD27 ++ PCs. Additionally, the presence of serum ANAs was not associated with abnormalities in other peripheral B-cell subsets. It remains to be established at which stage of SLE development the expansion of the PC compartment is initiated.

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Section: Methodsmentioning

confidence: 99%

Analyses of CD27++ Plasma Cells in Peripheral Blood from Patients with Bacterial Infections and Patients with Serum Antinuclear Antibodies

et al. 2007

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“… 10 11 Interestingly, patients with SLE have lower circulating frequencies of CD45RO-negative naïve CD4 T cells. 12 By directly measuring PBMC, increased frequencies of Th17 cells have been reported in SLE, but there is controversy over the correlation of Th17 status with disease activity and characterisation of the memory T cell markers is restricted. 3 4 In this study we were able to detect the Th17 population by using sorted high-purity CD45RO-positive CD4 T cells, and our findings clearly demonstrate increased Th17 frequencies with a positive correlation with disease activity and with the amount of proteinuria in patients with SLE.…”

Section: Discussionmentioning

confidence: 99%

Increased Th17 cells in flow cytometer-sorted CD45RO-positive memory CD4 T cells from patients with systemic lupus erythematosus

Liu

Wang

2014

Lupus Sci Med

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ObjectivesTh17/IL-17 dysregulation is involved in human autoimmunity, and recent evidence suggests the character of long-lived differentiated memory cells in Th17. By directly measuring the peripheral blood mononuclear cells (PBMC), elevated circulating frequencies of Th17 cells have been reported in systemic lupus erythematosus (SLE) with inconsistent results regarding the correlation with disease activities. In this study, the association between circulating Th17 frequencies and disease activities or laboratory parameters was examined in flow cytometer-sorted CD45RO-positive memory CD4 T cells from SLE.MethodsPBMC samples were obtained from 48 female lupus patients and another 48 age- and sex-matched healthy individuals. We examined frequencies of Th17 cells by sorting the purified CD4 T cells bearing the CD45RO marker, followed by intracellular IL-17A staining after in vitro activation. Frequencies of Th1 and TFoxp3 cells were also measured by intracellular IFN-γ and Foxp3 staining, respectively. The SLE disease activity index (SLEDAI) and other laboratory parameters were further correlated with frequencies of different T cell subsets.ResultsIn SLE, increased frequencies of Th17 cells were found with a positive correlation in SLEDAI. Higher frequencies of Th17 cells were found in lupus nephritis. There was a positive correlation between frequencies of Th17 cells and daily proteinuria amount.ConclusionsBy examining the sorted CD45RO-positive memory CD4 T cells, we confirm the dysregulation of Th17/IL-17 in SLE, implicating the potential to treat lupus patients with selective IL-17/IL-17R blockades.

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“…This will result in the adherence and subsequent migration of mononuelear cells, in particular when these cells are activated. Indeed, expression of activation markers is upregulated on circulating B and T cells already during quiescent SLE [6]. Before disease activity, this expression is further increased [25).…”

Section: Discissionmentioning

confidence: 99%

Levels of soluble VCAM-1, soluble ICAM-1, and soluble E-selectin during disease exacerbations in patients with systemic lupus erythematosus (SLE); a long term prospective study

Spronk¹,

Bootsma²,

Huitema³

et al. 1994

Clinical and Experimental Immunology

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130

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SUMMARYActive SLE is characterized by immune deposits and subsequent vascular inflammation in many organs. Expression and up-rcgulation of adhesion molecules is basic to migration of inflammatory cells into the tissues. Recently, soluble isoforms of these molecules have been described which might be an expression of their up-regulation in the tissues and, as such, of disease activity. The purpose of this study was to evaluate whether changes in levels of soluble adhesion molecules reflect disease activity. We analysed .serial sera in a 6-month period preceding 22 consecutive exacerbations of SLE for levels of soluble vascular cell adhesion molecule-l (sVCAM-1). soluble intercellular adhesion molecule-l (slCAM-1), and sE-selectin. Levels were related to clinical disease activity (SLEDAI). and levels of anti-dsDNA and complement. At the time of maximal disease activity, levels of sVCAM-1 in patients with SLE were higher than those in controls (A" < 00001), levels in patients with renal involvement being higher than in those without {P < 0 02). Levels of sVCAM-1 correlated with SLEDAI scores {P < 0 05) and, inversely, with levels of C3 (P = 0 01). In addition, in the presence of anti-dsDNA, levels of sVCAM-1 tended to correlate with levels of these autoantibodies (P < 01). Levels of sICAM-1 were normal and sEselectin levels even decreased compared with controls. Levels of sVCAM-l were higher at the moment of relapse {P = 0 001) than at 6 months before this time point. This rise correlated with the rise in SLEDAI score (f < 0 02). l.evelsof sICAM-l and sE-selectin did not rise, and remained in the normal range in all exacerbations studied. In conclusion, in contrast to sICAM-1 and sEselectin, levels of sVCAM-l are increased, rise parallel to disease activity during exacerbations in SLE, and are associated with decreasing levels of complement factors. This favours the hypothesis of immune deposit formation, activation of the complement cascade and activation of endothelial cells. Concurrent up-regulation of vascular adhesion molecules may thus result in transmigration of activated inflammatory cells inducing tissue damage.

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B cell activation in clinically quiescent systemic lupus erythematosus (SLE) is related to immunoglobulin levels, but not to levels of anti-dsDNA, nor to concurrent T cell activation

Cited by 25 publications

References 24 publications

Analyses of CD27++ Plasma Cells in Peripheral Blood from Patients with Bacterial Infections and Patients with Serum Antinuclear Antibodies

Analyses of CD27++ Plasma Cells in Peripheral Blood from Patients with Bacterial Infections and Patients with Serum Antinuclear Antibodies

Increased Th17 cells in flow cytometer-sorted CD45RO-positive memory CD4 T cells from patients with systemic lupus erythematosus

Levels of soluble VCAM-1, soluble ICAM-1, and soluble E-selectin during disease exacerbations in patients with systemic lupus erythematosus (SLE); a long term prospective study

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