B cell epitope mapping and characterization of naturally acquired antibodies to the Plasmodium vivax Merozoite Surface Protein-3α (PvMSP-3α) in malaria exposed individuals from Brazilian Amazon

Lima-Junior, Josué da Costa; Jiang, Jianlin; Rodrigues-da-Silva, Rodrigo Nunes; Banic, Dalma Maria; Tran, Tuan M.; Ribeiro, Rita; Meyer, V.S.E.; De-Simone, Salvatore Giovanni; Santos, Fernando J.; Moreno, Alberto; Barnwell, John W.; Galinski, Mary R.; Oliveira-Ferreira, Joseli

doi:10.1016/j.vaccine.2010.12.099

Cited by 44 publications

(47 citation statements)

References 66 publications

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“…The two cross-sectional studies gave opposing results and were not combined (I 2 > 75% and/or P < 0.01). Lima-Junior et al [25] showed around a 60% reduction in the odds of P. vivax infection in IgG responders to PvMSP-3α full-length (MSP-3α FL ), PvMSP-3α NT , PvMSP-3α RI , and around a 35% reduction for PvMSP-3α RII and PvMSP-3α CT compared with non-responders ( Figure 6). Conversely, Stanisic et al [36] showed fold increases of between Figure 5 Forest plot of the association of PvMSP-1 N-terminus IgG responses with Plasmodium vivax outcomes.…”

Section: Cross-sectional Studiesmentioning

confidence: 95%

“…The full texts of these 162 studies were examined to determine whether they complied with eligibility criteria: 114 did not meet the inclusion criteria (see Additional file 3), 7 fulfilled the inclusion and quality criteria (Figure 1), and 41 studies potentially met inclusion and quality criteria. The authors of the 41 studies that potentially met inclusion and quality criteria were contacted, yielding a further 11 studies that met inclusion and quality criteria, providing a total of 18 studies that were included in the review [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39] (Figure 1). Details of these 18 studies are shown in Table 1.…”

Section: Identification and Description Of Included Studiesmentioning

confidence: 99%

“…One cross-sectional study in Brazil by Lima-Junior et al [25] and one cohort study in PNG by Stanisic et al [36] (which contributed both cross-sectional and cohort data) investigated the association between antibody responses to regions of PvMSP-3α and P. vivax outcomes. The two cross-sectional studies gave opposing results and were not combined (I 2 > 75% and/or P < 0.01).…”

Section: Cross-sectional Studiesmentioning

confidence: 99%

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Immunological markers of Plasmodium vivax exposure and immunity: a systematic review and meta-analysis

Cutts¹,

Powell²,

Agius³

et al. 2014

BMC Medicine

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Background: Identifying Plasmodium vivax antigen-specific antibodies associated with P. vivax infection and protective immunity is key to the development of serosurveillance tools and vaccines for malaria. Antibody targets of P. vivax can be identified by seroepidemiological studies of individuals living in P. vivax-endemic areas, and is an important strategy given the limited ability to culture P. vivax in vitro. There have been numerous studies investigating the association between P. vivax antibody responses and P. vivax infection, but there has been no standardization of results to enable comparisons across populations. Methods: We performed a systematic review with meta-analysis of population-based, cross-sectional, case-control, and cohort studies of individuals living in P. vivax-endemic areas. We searched 6 databases and identified 18 studies that met predefined inclusion and quality criteria, and examined the association between antibody responses to P. vivax antigens and P. vivax malaria.

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Section: Cross-sectional Studiesmentioning

confidence: 95%

Section: Identification and Description Of Included Studiesmentioning

confidence: 99%

Section: Cross-sectional Studiesmentioning

confidence: 99%

See 1 more Smart Citation

Immunological markers of Plasmodium vivax exposure and immunity: a systematic review and meta-analysis

Cutts¹,

Powell²,

Agius³

et al. 2014

BMC Medicine

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“…Such initial observations of polymorphism, together with the fact that these proteins are immunogenic (Bitencourt et al, 2013; Lima-Junior et al, 2011, 2012; Mourão et al, 2012), are thought to indicate that the msp3 family has an important function in the RBC invasion process, possibly through a role in immune evasion (Jiang et al 2013). …”

Section: Introductionmentioning

confidence: 99%

The origin and diversification of the merozoite surface protein 3 (msp3) multi-gene family in Plasmodium vivax and related parasites

Rice

Acosta

Pacheco

et al. 2014

Molecular Phylogenetics and Evolution

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The genus Plasmodium is a diversified group of parasites with more than 200 known species that includes those causing malaria in humans. These parasites use numerous proteins in a complex process that allows them to invade the red blood cells of their vertebrate hosts. Many of those proteins are part of multi-gene families; one of which is the merozoite surface protein-3 (msp3) family. The msp3 multi-gene family is considered important in the two main human parasites, Plasmodium vivax and Plasmodium falciparum, as its paralogs are simultaneously expressed in the blood stage (merozoite) and are immunogenic. There are large differences among Plasmodium species in the number of paralogs in this family. Such differences have been previously explained, in part, as adaptations that allow the different Plasmodium species to invade their hosts. To investigate this, we characterized the array containing msp3 genes among several Plasmodium species, including P. falciparum and P. vivax. We first found no evidence indicating that the msp3 family of P. falciparum was homologous to that of P. vivax. Subsequently, by focusing on the diverse clade of nonhuman primate parasites to which P. vivax is closely related, where homology was evident, we found no evidence indicating that the interspecies variation in the number of paralogs was an adaptation related to changes in host range or host switches. Overall, we hypothesize that the evolution of the msp3 family in P. vivax is consistent with a model of multi-allelic diversifying selection where the paralogs may have functionally redundant roles in terms of increasing antigenic diversity. Thus, we suggest that the expressed MSP3 proteins could serve as “decoys”, via antigenic diversity, during the critical process of invading the host red blood cells.

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“…We previously used a similar method to compare sera from mice immunized with one PspA variant as a recombinant protein and as a DNA vaccine (34). This technique has also been used successfully to screen epitopes of antigens from other pathogens (35,36). The present study tested sera from mice immunized with several variants of PspA and PspC3 to identify the most immunogenic epitopes.…”

mentioning

confidence: 99%

Mapping of Epitopes Recognized by Antibodies Induced by Immunization of Mice with PspA and PspC

Vadesilho

Ferreira

Gordon

et al. 2014

Clin. Vaccine Immunol.

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cPneumococcal surface protein A (PspA) and pneumococcal surface protein C (PspC) are important candidates for an alternative vaccine against pneumococcal infections. Since these antigens show variability, the use of variants that do not afford broad protection may lead to the selection of vaccine escape bacteria. Epitopes capable of inducing antibodies with broad cross-reactivities should thus be the preferred antigens. In this work, experiments using peptide arrays show that most linear epitopes recognized by antibodies induced in mice against different PspAs were located at the initial 44 amino acids of the mature protein and that antibodies against these linear epitopes did not confer protection against a lethal challenge. Conversely, linear epitopes recognized by antibodies to PspC included the consensus sequences involved in the interaction with human factor H and secretory immunoglobulin A (sIgA). Since linear epitopes of PspA were not protective, larger overlapping fragments containing 100 amino acids of PspA of strain Rx1 were constructed (fragments 1 to 7, numbered from the N terminus) to permit the mapping of antibodies with conformational epitopes not represented in the peptide arrays. Antibodies from mice immunized with fragments 1, 2, 4, and 5 were capable of binding onto the surface of pneumococci and mediating protection against a lethal challenge. The fact that immunization of mice with 100-amino-acid fragments located at the more conserved N-terminal region of PspA (fragments 1 and 2) induced protection against a pneumococcal challenge indicates that the induction of antibodies against conformational epitopes present at this region may be important in strategies for inducing broad protection against pneumococci.

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B cell epitope mapping and characterization of naturally acquired antibodies to the Plasmodium vivax Merozoite Surface Protein-3α (PvMSP-3α) in malaria exposed individuals from Brazilian Amazon

Cited by 44 publications

References 66 publications

Immunological markers of Plasmodium vivax exposure and immunity: a systematic review and meta-analysis

Immunological markers of Plasmodium vivax exposure and immunity: a systematic review and meta-analysis

The origin and diversification of the merozoite surface protein 3 (msp3) multi-gene family in Plasmodium vivax and related parasites

Mapping of Epitopes Recognized by Antibodies Induced by Immunization of Mice with PspA and PspC

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