Immunological markers of Plasmodium vivax exposure and immunity: a systematic review and meta-analysis

Cutts, Julia C; Powell, Rosanna; Agius, Paul A.; Beeson, James G.; Simpson, Julie A.; Fowkes, Freya J I

doi:10.1186/preaccept-1041462276129106

Cited by 34 publications

(47 citation statements)

References 60 publications

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“…The response to the highly conserved phosphoriboprotein peptide P2 was similar to that observed for MSP‐1 and better than that of AMA‐1, suggesting P2's potential as a diagnostic candidate. Both anti‐MSP‐1 and anti‐AMA‐1 responses are generally found to correlate well with vivax infection . We found a 50% positivity rate for these antigens in our cohort.…”

Section: Discussionsupporting

confidence: 54%

Vivax infection alters peripheral B‐cell profile and induces persistent serum IgM

Patgaonkar

Herbert

Powale

et al. 2018

Parasite Immunology

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B cell-mediated humoral responses are essential for controlling malarial infection. Studies have addressed the effects of Plasmodium falciparum infection on peripheral B-cell subsets but not much is known for P. vivax infection. Furthermore, majority of the studies investigate changes during acute infection, but not after parasite clearance. In this prospective study, we analysed peripheral B-cell profiles and antibody responses during acute P. vivax infection and upon recovery (30 days post-treatment) in a low-transmission area in India. Dengue patients were included as febrile-condition controls. Both dengue and malaria patients showed a transient increase in atypical memory B cells during acute infection. However, transient B cell-activating factor (BAFF)-independent increase in the percentage of total and activated immature B cells was observed in malaria patients. Naïve B cells from malaria patients also showed increased TLR4 expression. Total IgM levels remained unchanged during acute infection but increased significantly at recovery. Serum antibody profiling showed a parasite-specific IgM response that persisted at recovery. A persistent IgM autoantibody response was also observed in malaria but not dengue patients. Our data suggest that in hypoendemic regions acute P. vivax infection skews peripheral B-cell subsets and results in a persistent parasite-specific and autoreactive IgM response.

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Section: Discussionsupporting

confidence: 54%

Vivax infection alters peripheral B‐cell profile and induces persistent serum IgM

Patgaonkar

Herbert

Powale

et al. 2018

Parasite Immunology

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“…Indeed, a meta-analysis study discuss that despite a great heterogeneity of humoral response observed where P . vivax infection is endemic, IgG response to a number of antigens is associated with increase antibody levels [46]. The same is observed when the dynamics of the antibody response is assessed during P .…”

Section: Discussionmentioning

confidence: 93%

T follicular helper cells regulate the activation of B lymphocytes and antibody production during Plasmodium vivax infection

et al. 2017

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Although the importance of humoral immunity to malaria has been established, factors that control antibody production are poorly understood. Follicular helper T cells (Tfh cells) are pivotal for generating high-affinity, long-lived antibody responses. While it has been proposed that expansion of antigen-specific Tfh cells, interleukin (IL) 21 production and robust germinal center formation are associated with protection against malaria in mice, whether Tfh cells are found during Plasmodium vivax (P. vivax) infection and if they play a role during disease remains unknown. Our goal was to define the role of Tfh cells during P. vivax malaria. We demonstrate that P. vivax infection triggers IL-21 production and an increase in Tfh cells (PD-1+ICOS+CXCR5+CD45RO+CD4+CD3+). As expected, FACS-sorted Tfh cells, the primary source of IL-21, induced immunoglobulin production by purified naïve B cells. Furthermore, we found that P. vivax infection alters the B cell compartment and these alterations were dependent on the number of previous infections. First exposure leads to increased proportions of activated and atypical memory B cells and decreased frequencies of classical memory B cells, whereas patients that experienced multiple episodes displayed lower proportions of atypical B cells and higher frequencies of classical memory B cells. Despite the limited sample size, but consistent with the latter finding, the data suggest that patients who had more than five infections harbored more Tfh cells and produce more specific antibodies. P. vivax infection triggers IL-21 production by Tfh that impact B cell responses in humans.

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“…Antibodies are important biomarkers in a broad range of diseases, and especially important for the diagnosis and surveillance of infectious diseases, autoimmune diseases and allergies. [1][2][3][4] In addition, antibody-based drug therapies constitute an important part of newly introduced drugs, in particular in the field of oncology and inflammatory diseases. 5,6 While a wide variety of sensitive antibody detection strategies have been developed, many of them come with intrinsic limitations such as the requirement for multiple time-consuming incubation steps (ELISA), multiple reagents or sophisticated equipment (Luminex, SPR).…”

mentioning

confidence: 99%

Detection of Antibodies in Blood Plasma Using Bioluminescent Sensor Proteins and a Smartphone

et al. 2016

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Antibody detection is of fundamental importance in many diagnostic and bioanalytical assays, yet current detection techniques tend to be laborious and/or expensive. We present a new sensor platform (LUMABS) based on bioluminescence resonance energy transfer (BRET) that allows detection of antibodies directly in solution using a smartphone as the sole piece of equipment. LUMABS are single-protein sensors that consist of the blue-light emitting luciferase NanoLuc connected via a semiflexible linker to the green fluorescent acceptor protein mNeonGreen, which are kept close together using helper domains. Binding of an antibody to epitope sequences flanking the linker disrupts the interaction between the helper domains, resulting in a large decrease in BRET efficiency. The resulting change in color of the emitted light from green-blue to blue can be detected directly in blood plasma, even at picomolar concentrations of antibody. Moreover, the modular architecture of LUMABS allows changing of target specificity by simple exchange of epitope sequences, as demonstrated here for antibodies against HIV1-p17, hemagglutinin (HA), and dengue virus type I. The combination of sensitive ratiometric bioluminescent detection and the intrinsic modularity of the LUMABS design provides an attractive generic platform for point-of-care antibody detection that avoids the complex liquid handling steps associated with conventional immunoassays.

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Immunological markers of Plasmodium vivax exposure and immunity: a systematic review and meta-analysis

Cited by 34 publications

References 60 publications

Vivax infection alters peripheral B‐cell profile and induces persistent serum IgM

Vivax infection alters peripheral B‐cell profile and induces persistent serum IgM

T follicular helper cells regulate the activation of B lymphocytes and antibody production during Plasmodium vivax infection

Detection of Antibodies in Blood Plasma Using Bioluminescent Sensor Proteins and a Smartphone

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