Comparison between the phenotype and function of maturing dendritic cells from spleen and lymph nodes

Abstract

SUMMARYWe compared the capacity of mature dendritic cells (DC) from lymph nodes and maturing DC from spleens in their capacity to stimulate responses to the small hapten picryl sulphonic acid (PIC) and to the same hapten conjugated to ovalbumin (PIC-OVA) and requiring processing. Surface expression of major histocompatibility complex (MHC) class II molecules, which are up-regulated during maturation of splenic DC, were studied as an independent marker of maturation. Freshly isolated lymph node DC had a veiled appearance and high levels of class II expression. DC separated from suspensions of spleen cells expressed the DC-specific marker NLDC-145, but were small, had low levels of MHC class II molecules and expressed stem cell antigen. Those DC from spleen cells cultured for 24 and 48 hr showed the development of typical veiled DC morphology and high class II expression. Lymph node DC stimulated high levels of primary T-cell proliferation to PIC, but failed to stimulate primary responses to PIC-OVA. Splenic DC isolated immediately failed to stimulate primary responses to either antigen. More mature spleen DC stimulated responses both to PIC and PIC-OVA. Surprisingly, development of the capacity to stimulate responses to PIC preceded that of stimulating PIC-OVA responses. The capacity of the DC to process and present PIC-OVA was maintained during the culture period. The results indicate that both the form of the antigen and the source and maturity of the DC are critical in determining the responses stimulated in T lymphocytes.

Adjuvant is the Major Parameter Influencing the Isotype Profiles Generated During Immunization with a Protein Antigen, the Schistosoma mansoni Sm28‐GST

“…These observations are consistent with previous studies showing that dendritic cells in the skin, through IL-12 production, promote Th1 responses [37], and that peritoneal macrophages promote type 2 responses [38]. Moreover, some evidence suggests that lymph nodes and spleen cells are functionally different [39,40]. Finally, Th1 and Th2 cells may present differences in migratory 5.…”

Murine dendritic cell development: Difficulties associated with subset analysis

“…Electron microscopic features include prominent mitochondria and the presence of endosomes and lysosomes essential for antigen processing 21 . Murine DC are defined by their expression of a range of cell surface markers including CD11c, 22 CD11b, 23 , 24 CD205, 25 , 26 , 27 MHC class II 28 , 29 and 33D1 30 …”