<b>Isolation and mapping of polymorphic microsatellites in cattle</b>

Steffen, P.; Eggen, Andre A.; Dietz, Allan B.; Womack, James E.; Stranzinger, G.; Fries, R.

doi:10.1111/j.1365-2052.1993.tb00252.x

Cited by 147 publications

(35 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Panel 1 included primers INRA011 [23], Cervid1 [24], ILSTS011 (0.5 µM) [25], OCAM [26] and BovPRL (1 µM) [27]. Panel 2 included N, Q [28], ETH152 [29] and BM203 (0.5 µM) [30]. Panel 3 included K [28], BL25, BM6438, BM848 [30] and O (0.5 µM) [28].…”

Section: Methodsmentioning

confidence: 99%

Heterozygosity in an Isolated Population of a Large Mammal Founded by Four Individuals Is Predicted by an Individual-Based Genetic Model

Kekkonen

Wikström²,

Brommer

2012

PLoS ONE

View full text Add to dashboard Cite

BackgroundWithin-population genetic diversity is expected to be dramatically reduced if a population is founded by a low number of individuals. Three females and one male white-tailed deer Odocoileus virginianus, a North American species, were successfully introduced in Finland in 1934 and the population has since been growing rapidly, but remained in complete isolation from other populations.Methodology/Principal FindingsBased on 14 microsatellite loci, the expected heterozygosity H was 0.692 with a mean allelic richness (AR) of 5.36, which was significantly lower than what was found in Oklahoma, U.S.A. (H = 0.742; AR = 9.07), demonstrating that a bottleneck occurred. Observed H was in line with predictions from an individual-based model where the genealogy of the males and females in the population were tracked and the population's demography was included.ConclusionOur findings provide a rare within-population empirical test of the founder effect and suggest that founding a population by a small number of individuals need not have a dramatic impact on heterozygosity in an iteroparous species.

show abstract

Section: Methodsmentioning

confidence: 99%

Heterozygosity in an Isolated Population of a Large Mammal Founded by Four Individuals Is Predicted by an Individual-Based Genetic Model

Kekkonen

Wikström²,

Brommer

2012

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…The markers used were BM1824 (Bishop and Kappes, 1994), BM2113 (Bishop and Kappes, 1994), ETH10 (Toldo and Fries, 1993), ETH225 (Steffen and Eggen, 1993), INRA023 (Vaiman and Mercier, 1994), SPS115 (Moore and Byrne, 1994), TGLA53 (Kappes et al , 1997), TGLA122 (Barendse and Armitage, 1994), TGLA126 (Kappes et al , 1997) and TGLA227 (Kappes et al , 1997). The microsatellite markers were grouped into one multiplex PCR reaction, and primers were labelled with fluorescent markers of three colours to distinguish between fragments of a similar size.…”

mentioning

confidence: 99%

Implementation of a parentage control system in Portuguese beef-cattle with a panel of microsatellite markers

Carolino¹,

Sousa²,

Ferreira³

et al. 2009

Genet. Mol. Biol.

View full text Add to dashboard Cite

A study was conducted to assess the feasibility of applying a panel of 10 microsatellite markers in parentage control of beef cattle in Portugal. In the first stage, DNA samples were collected from 475 randomly selected animals of the Charolais, Limousin and Preta breeds. Across breeds and genetic markers, means for average number of alleles, effective number of alleles, expected heterozygosity and polymorphic information content, were 8.20, 4.43, 0.733 and 0.70, respectively. Enlightenment from the various markers differed among breeds, but the set of 10 markers resulted in a combined probability above 0.9995 in the ability to exclude a random putative parent. The marker-set thus developed was later used for parentage control in a group of 140 calves from several breeds, where there was the suspicion of possible faulty parentage recording. Overall, 76.4% of the calves in this group were compatible with the recorded parents, with most incompatibilities due to misidentification of the dam. Efforts must be made to improve the quality of pedigree information, with particular emphasis on information recorded at the calf's birth.

show abstract

“…First, we tested 38 microsatellite loci that have been successfully amplified in other hoofed species: Bos taurus-BM1258 (Hediger et al 1991), BovirBP, MM12 (Moore et al 1992), MAF46 (Swarbrick et al 1992), INRA003 (Vaiman et al 1992), INRA35 (Steffen et al 1993), TGLA53 , BL42, BM1225, BM2830, BM4513, BM861, BM888, BMC1009, IOBT965 (Bishop et al 1994), CSSM003 , INRA124 (Vaiman et al 1994), Haut14 (Thieven et al 1995), INRA189 (Kappes et al 1997), UMN2404 (Liu et al 2002), and UMN2001 (Liu et al 2003); Cervus elaphus-CelJP15 (Pemberton et al 1995); Odocoileus virginianus-Cer14 (DeWoody et al 1995); Ovis aries-MAF70 (Buchanan and Crawford 1992), OarFCB193, OarFCB304 (Buchanan and Crawford 1993), OarFCB20 (Buchanan et al 1994), McM58, andMcM130 (Hulme et al 1994); and Rangifer tarandus-RT5, RT9, RT23, RT24, RT27, RT30 (Wilson et al 1997), NVHR T01, NVHRT21, and NVHRT24 (Røed and Midthjell 1998) (Table 5S1 and Table 6S2). Markers were selected on the basis of their previous cross-species utility, size range, and ability to co-amplify in multiplex PCRs.…”

Section: Genotypingmentioning

confidence: 99%

Admixture promotes genetic variation in bottlenecked moose populations in eastern Poland

et al. 2015

View full text Add to dashboard Cite

Human activity has led to severe bottlenecks in many wildlife species in the recent past. This usually increases the strength of genetic drift, leading to loss of genetic variation. Gene flow may however counteract the genetic consequences of small population size. Using 11 of 38 tested microsatellite loci and five moose populations in eastern Poland, we investigated the effects of two phenomena: bottlenecks that occurred in the nineteenth century and the first half of twentieth century, and admixture after moose populations expanded demographically and spatially in eastern Poland after the Second World War. The statistical tests indicated a recent bottleneck in all the studied samples with respect to H E and low Garza-Williamson index values. The Biebrza population, which consists of autochthonous moose representing a branch of the Central Europe mitochondrial DNA (mtDNA) clade and immigrants belonging to the Ural clade, is one of the most variable populations of this species. AMOVA, PCA, and STRUCTURE analyses all revealed significant population structuring, with most probable existence of K=2 genetically distinct clusters that exhibited a relatively high level of admixture. Analysis of recent dispersal rates demonstrated that population from the Biebrza Valley may supply individuals to the other four studied moose populations. We also found female-biased sex ratio in nonharvested moose populations inhabiting eastern Poland.

show abstract

Isolation and mapping of polymorphic microsatellites in cattle

Cited by 147 publications

References 13 publications

Heterozygosity in an Isolated Population of a Large Mammal Founded by Four Individuals Is Predicted by an Individual-Based Genetic Model

Heterozygosity in an Isolated Population of a Large Mammal Founded by Four Individuals Is Predicted by an Individual-Based Genetic Model

Implementation of a parentage control system in Portuguese beef-cattle with a panel of microsatellite markers

Admixture promotes genetic variation in bottlenecked moose populations in eastern Poland

Contact Info

Product

Resources

About