B subunit of<i>Escherichia coli</i>heat-labile enterotoxin as adjuvant of humoral immune response in recombinant BCG vaccination

Rocha, Aline; Conceição, Fabrı́cio R.; Grassmann, André Alex; Lagranha, Valeska Lizzi; Dellagostin, Odir A.

doi:10.1139/w08-056

Cited by 16 publications

(13 citation statements)

References 44 publications

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“…As is the case with CT-B, the LT-B subunit is a less potent adjuvant than the LT holotoxin, but in many instances it has been shown to potentiate the response after mucosal immunization against inactivated virus, viral glycoproteins, and bacterial proteins; LT-B is also known to be an effective carrier in fusion proteins (Clements, 1990;Sanchez et al, 1986;O'dowd et al, 1999;Da Silva Ramos Rocha et al, 2008;Fingerut et al, 2005).…”

Section: E Coli Heat-labile Toxinmentioning

confidence: 99%

Mucosal Adjuvants

Freytag

Clements

2015

Mucosal Immunology

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Section: E Coli Heat-labile Toxinmentioning

confidence: 99%

Mucosal Adjuvants

Freytag

Clements

2015

Mucosal Immunology

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“…The B subunit of Escherichia coli heat-labile enterotoxin (LTB) is highly immunogenic upon systemic (6,9,15) and mucosal (14,37) immunizations. Its adjuvant activity has been demonstrated with unrelated antigens, both coadministered (14,15) and linked by chemical conjugation or genetic fusion (6,14,37), exhibiting no toxic effect (8).…”

mentioning

confidence: 99%

Protection against Lethal Leptospirosis after Vaccination with LipL32 Coupled or Coadministered with the B Subunit of Escherichia coli Heat-Labile Enterotoxin

Grassmann

Félix

Santos

et al. 2012

Clin. Vaccine Immunol.

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ABSTRACTLeptospirosis, a worldwide zoonosis, lacks an effective, safe, and cross-protective vaccine. LipL32, the most abundant, immunogenic, and conserved surface lipoprotein present in all pathogenic species ofLeptospira, is a promising antigen candidate for a recombinant vaccine. However, several studies have reported a lack of protection when this protein is used as a subunit vaccine. In an attempt to enhance the immune response, we used LipL32 coupled to or coadministered with the B subunit of theEscherichia coliheat-labile enterotoxin (LTB) in a hamster model of leptospirosis. After homologous challenge with 5× the 50% lethal dose (LD50) ofLeptospira interrogans, animals vaccinated with LipL32 coadministered with LTB and LTB::LipL32 had significantly higher survival rates (P< 0.05) than animals from the control group. This is the first report of a protective immune response afforded by a subunit vaccine using LipL32 and represents an important contribution toward the development of improved leptospirosis vaccines.

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“…The CT from Vibrio cholerae and LT from enterotoxigenic strains of E.coli are potent mucosal adjuvants. It has been found that CT induces a Th2 response (26), whereas LT induces a Th1‐biased immune response (27, 28). The B subunits of these toxins are less potent than the active toxins.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Helicobacter pylori outer inflammatory protein DNA vaccine efficacy by co‐delivery of interleukin‐2 and B subunit heat‐labile toxin gene encoded plasmids

Chen

Lin

et al. 2012

Microbiology and Immunology

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Development of an effective vaccine for controlling H. pylori-associated infection, which is present in about half the people in the world, is a priority. The H. pylori outer inflammatory protein (oipA) has been demonstrated to be a potential antigen for a vaccine. In the present study, use of oipA gene encoded construct (poipA) for C57BL/6 mice vaccination was investigated. Whether co-delivery of IL-2 gene encoded construct (pIL-2) and B subunit heat-labile toxin of Escherichia coli gene encoded construct (pLTB) can modulate the immune response and enhance DNA vaccine efficacy was also explored. Our results demonstrated that poipA administered intradermally ('gene gun' immunization) promoted a strong Th2 immune response, whereas co-delivery of either pIL-2 or pLTB adjuvant elicited a Th1-biased immune response. PoipA administered with both pIL-2 and pLTB adjuvants promoted a strong Th1 immune response. Regardless of the different immune responses promoted by the various vaccination regimes, all immunized mice had smaller bacterial loads after H. pylori challenge than did PBS negative and pVAX1 mock controls. Co-delivery of adjuvant(s) enhances poipA DNA vaccine efficacy by shifting the immune response from being Th2 to being Th1-biased, which results in a greater reduction in bacterial load after H. pylori challenge. Both prophylactic and therapeutic vaccination can achieve sterile immunity in some subjects.

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B subunit ofEscherichia coliheat-labile enterotoxin as adjuvant of humoral immune response in recombinant BCG vaccination

Cited by 16 publications

References 44 publications

Mucosal Adjuvants

Mucosal Adjuvants

Protection against Lethal Leptospirosis after Vaccination with LipL32 Coupled or Coadministered with the B Subunit of Escherichia coli Heat-Labile Enterotoxin

Enhancement of Helicobacter pylori outer inflammatory protein DNA vaccine efficacy by co‐delivery of interleukin‐2 and B subunit heat‐labile toxin gene encoded plasmids

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