<b>β‐Carboline Alkaloids as Matrices for Matrix‐assisted Ultraviolet Laser Desorption Time‐of‐flight Mass Spectrometry of Proteins and Sulfated Oligosaccharides: a Comparative Study Using Phenylcarbonyl Compounds, Carbazoles and Classical Matrices</b>

Nonami, Hiroshi; Fukui, Shinsaku; Erra‐Balsells, Rosa

doi:10.1002/(sici)1096-9888(199703)32:3<287::aid-jms478>3.0.co;2-#

Cited by 103 publications

(92 citation statements)

References 2 publications

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“…As part of a project related with the study of the thermal and photochemical properties of current used organic crystalline MALDI matrixes (Mesaros et al, 2006;Tarzi et al, 2009;Cole, 2010;Petroselli et al, 2012) in order to improve MALDI MS analysis and to develop new matrices (Nonami et al, 1997;Nonami et al, 1998;Landoni et al, 2008;Gholipour et al, 2010) several ILs were prepared combining commercially available trans-cinnamic acids trans-CH) (Fig. 9, i.e., trans-FA) and an organic bases (Am) (Fig.…”

Section: One-pot Cis-cinnamic Acids Synthesis By Photoisomerization Omentioning

confidence: 99%

High Purity cis-Cinnamic Acid Preparation for Studying Physiological Role of trans-Cinnamic and cis-Cinnamic Acids in Higher Plants

Salum

Erra‐Balsells

2013

Environmental Control in Biology

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Cinnamic acids are present in all kinds of plant tissues and as consequence in herbs and derived medicines, cosmetics, foods, etc. The interest in study their role in nature and possible health effect on consumers has grown exponentially. Because of their molecular structure they can exist in both trans-and cis-forms, having both found in plants. However, as only the trans-forms are commercially available very few studies in vitro and in vivo of the cis-role are available. In the present review the current knowledge in this field of studies is summarized including the brief description of a new tool for easy and friendly synthesis of cis-cinnamic acids by researchers in order to be able to conduct new experiments.

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Section: One-pot Cis-cinnamic Acids Synthesis By Photoisomerization Omentioning

confidence: 99%

High Purity cis-Cinnamic Acid Preparation for Studying Physiological Role of trans-Cinnamic and cis-Cinnamic Acids in Higher Plants

Salum

Erra‐Balsells

2013

Environmental Control in Biology

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“…The above limitations potentially can be resolved by using negative ion mode MALDI MS analysis of oligosaccharides, which has been gaining popularity after a series of ␤-carboline compounds, e.g., harmane, nonharmane, and harmine, were introduced as efficient MALDI matrixes [31][32][33]. The tendencies for fucosylated oligosaccharides to lose fucose and for sialylated oligosaccharides to lose sialic acid, as seen in most positive ion MALDI spectra, are not as apparent in negative ion mode analysis [34].…”

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confidence: 99%

MALDI linear-field reflectron TOF post-source decay analysis of underivatized oligosaccharides: Determination of glycosidic linkages and anomeric configurations using anion attachment

Guan

Cole

2008

J. Am. Soc. Mass Spectrom.

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Six different anionic species (fluoride, chloride, bromide, iodide, nitrate, and acetate) are tested for their abilities to form anionic adducts with neutral oligosaccharides that are detectable by MALDI-TOF mass spectrometry. Fluoride and acetate cannot form anionic adducts with the oligosaccharides in significant yields. However, bromide, iodide, and nitrate anionic adducts consistently appear in higher abundances relative to [M Ϫ H] Ϫ , just like the highly stable chloride adducts. Post-source decay (PSD) decompositions of Br Ϫ , IϪ , and NO 3 Ϫ adducts of oligosaccharides provide no structural information, i.e., they yield the respective anions as the main product ions. However, determination of linkage types is achieved by analysis of structurally-informative diagnostic peaks offered by negative ion PSD spectra of chloride adducts of oligosaccharides, whereas the relative peak intensities of pairs of diagnostic fragment ions allow differentiation of anomeric configurations of glycosidic bonds. Thus, simultaneous identification of the linkage types and anomeric configurations of glycosidic bonds is achieved. Our data indicate that negative ion PSD fragmentation patterns of chloride adducts of oligosaccharides are mainly determined by the linkage types. Correlation may exist between the linkage positions and fragmentation mechanisms and/or steric requirements for both cross-ring and glycosidic bond fragmentations. PSD of the chloride adducts of saccharides containing a terminal Glc␣1-2Fru linkage also yields chlorine-containing fragment ions which appear to be specifically diagnostic for a fructose linked at the 2-position on the reducing end. This also allows differentiation from saccharides with a 1-1 linked pyranose on the same position. ollowing the advances in proteomics, there is a growing interest in the importance of glycomics, i.e., the study of the breadth of sugar forms (structure and function of glycans) in biological organisms. Carbohydrates (or saccharides) are the most abundant biological compounds found on earth. They are well known as energy reservoirs and structural materials in cell walls. However, it has become clear that oligosaccharides and glycoconjugates (e.g., glycoproteins and glycolipids) serve as crucial mediators for a wide variety of complex cellular events [1]. Carbohydrates also play an important role in specific molecular recognition, protein folding, stability, and pharmacokinetics due to their great structural diversity. Moreover, glycosylation is a ubiquitous form of post-translational modification to both proteins and lipids.Gaining a clear understanding of the crucial biological roles of oligosaccharides requires complete structural characterization of carbohydrates or glycoconjugates, which includes determinations of the numbers and types of monosaccharide units, ring substituents, sequences, branching, linkage positions, and anomeric configurations between adjacent monosaccharide units. In most cases, merely knowing the monosaccharide sequence is inadequate; thus, unam...

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“…We have examined 18) some common organic matrices already introduced by other authors for the UV-MALDI MS of biomolecules including THAP 34) and DHB 35) as well as 9H-pyrido [3,4-b] indole (norHo) and extensively studied its properties and applications, [36][37][38][39][40][41] for their usefulness in plant single-cell metabolite pro ling. Additionally, we have introduced a number of new matrices for the UV-MALDI MS metabolite pro ling of plants including the nanoparticles (NPs) of titanium silicon oxide ((SiO 2 )(TiO 2 )), barium strontium titanium oxide ((BaTiO 3 )(SrTiO 3 )), titanium oxide (TiO 2 ), 42) and carbon nanotubes (CNTs).…”

Section: In Situ Pressure Probe Sampling and Uv-maldi Ms For Profilinmentioning

confidence: 99%

In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells

Gholipour¹,

Erra‐Balsells²,

Nonami³

2012

Mass Spectrometry

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β‐Carboline Alkaloids as Matrices for Matrix‐assisted Ultraviolet Laser Desorption Time‐of‐flight Mass Spectrometry of Proteins and Sulfated Oligosaccharides: a Comparative Study Using Phenylcarbonyl Compounds, Carbazoles and Classical Matrices

Cited by 103 publications

References 2 publications

High Purity cis-Cinnamic Acid Preparation for Studying Physiological Role of trans-Cinnamic and cis-Cinnamic Acids in Higher Plants

High Purity cis-Cinnamic Acid Preparation for Studying Physiological Role of trans-Cinnamic and cis-Cinnamic Acids in Higher Plants

MALDI linear-field reflectron TOF post-source decay analysis of underivatized oligosaccharides: Determination of glycosidic linkages and anomeric configurations using anion attachment

In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells

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