Ba/Mg co-doped hydroxyapatite/PLGA composites enhance X-ray imaging and bone defect regeneration

Liu, Xiang-Ji; Ma, Yihang; Chen, Minjiang; Ji, Jiansong; Zhu, Yuhang; Zhu, Qiang; Guo, Min; Zhang, Peibiao

doi:10.1039/d1tb01080h

Cited by 30 publications

(18 citation statements)

References 44 publications

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“…The co-doping of them makes the change of the HA crystal structure less altered. Liu et al (2021 ) also reported a similar phenomenon that the doping of Mg 2+ with a larger ionic radius balanced the effect of incorporation of Ba 2+ with a smaller ionic radius on the HA crystal structure.…”

Section: Resultsmentioning

confidence: 68%

“…During the synthetic process, those Ca 2+ ions could be replaced by other cations. It is reported that both Ca (I) and Ca (II) can be replaced by rare earth element ions and alkali earth metal ions, such as Mg 2+ , Fe 2+ , Sr 2+ , Ba 2+ , Na + , K + , and Ag + cations, which lead to new performance of HA ( Vukomanović et al, 2011 ; Fihri et al, 2017 ; Malakooti et al, 2018 ; Wang et al, 2020 ; Liu et al, 2021 ). Until now, to the best of our knowledge, there were no reports on hydrothermal synthesis of Ce 3+ and Gd 3+ co-doped HA NPs.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of Ce/Gd@HA/PLGA Scaffolds Contributing to Bone Repair and MRI Enhancement

Song

et al. 2022

Front. Bioeng. Biotechnol.

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It is important for future clinical applications to design and synthesize multipurpose scaffolding materials for bone tissue engineering with high osteogenic induction and MRI capability. In the present study, we synthesized Ce/Gd@HA by co-doping Ce3+ and Gd3+ into hydroxyapatite (HA) using a hydrothermal synthesis method, and then Ce/Gd@HA composites were synthesized by combining Ce/Gd@HA nanoparticles with polylactic-co-glycolic acid (PLGA) to investigate whether implanted Ce/Gd@HA/PLGA composites could promote osteoblast viability, leading to tibia repair of the rats and enhance MRI. The measurement results contain X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, and environmental scanning electron microscopy (ESEM) showing that HA doped with Ce3+ and Gd3+ was still a hexagonal crystal with high crystallinity. The synthesized Ce/Gd@HA/PLGA composites have a structure and obvious magnetic resonance imaging (MRI) capability. The in vitro experimental results indicated that Ce/Gd@HA/PLGA composites significantly promoted the performance of MC3T3-E1 cells, containing proliferation, adhesion, and osteogenic differentiation capacities. These include the improvement of alkaline phosphatase activity, enhancement of mineral deposition, and upregulation of OCN and COL-1 gene expression. The in vivo experimental results demonstrated that the Ce/Gd@HA/PLGA composites significantly improved the healing rate of rat bone defects. The MRI images indicated that the Ga-doped composites were observed in the MRI T1 sequence in rats. The aforementioned results suggested that Ce/Gd@HA/PLGA composites not only effectively promoted bone formation but also enhanced MRI capability. The composites synthesized in this study have great potential in bone regeneration with an extensive application in bone tissue engineering.

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Section: Resultsmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Synthesis of Ce/Gd@HA/PLGA Scaffolds Contributing to Bone Repair and MRI Enhancement

Song

et al. 2022

Front. Bioeng. Biotechnol.

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“…Noninvasive in vivo imaging technologies provide a powerful tool to guide and trace biomaterials in clinics. , Currently, there are several imaging modalities in medical fields, such as X-ray imaging, , magnetic resonance imaging (MRI), , fluorescence imaging, and so on, which differ from each other in imaging principles, resolution, sensitivity, depth, etc. Among these modalities, X-ray imaging is appropriate for deep-tissue imaging.…”

Section: Introductionmentioning

confidence: 99%

A Facile Composite Strategy to Prepare a Biodegradable Polymer Based Radiopaque Raw Material for “Visualizable” Biomedical Implants

Wang

Chen

et al. 2022

ACS Appl. Mater. Interfaces

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Enabling a biodegradable polymer radiopaque under X-ray is much desired for many medical devices. Physical blending of a present biodegradable polymer and a commercialized medical contrast agent is convenient yet lacks comprehensive fundamental research. Herein, we prepared a biodegradable polymer-based radiopaque raw material by blending poly(L-lactic acid) (PLLA or simply PLA) and iohexol (IHX), where PLA constituted the continuous phase and IHX particles served as the dispersed phase. The strong X-ray adsorption of IHX enabled the composite radiopaque; the hydrolysis of the polyester and the water solubility of the contrast agent enabled the composite biodegradable in an aqueous medium. The idea was confirmed by in vitro characterizations of the resultant composite, in vivo subcutaneous implantation in rats up to 6 months, and the clear visualization of a part of a biodegradable occluder in a Bama piglet under X-ray. We also found that the crystallization of PLA was significantly enhanced in the presence of the solid particles, which should be taken into consideration in the design of an appropriate biomaterial composite because crystallization degree influences the biodegradation rate and mechanical property of a material to a large extent. We further tried to introduce a small amount of poly(vinylpyrrolidone) into the blend of PLA and IHX. Compared to the bicomponent composite, the tricomponent one exhibited decreased modulus and increased elongation at break and tensile strength. This paves more ways for researchers to select appropriate raw materials according to the regenerated tissue and the application site.

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“…Poly lactide- co -glycolide (PLGA) scaffold has good biomechanical properties and controllable biodegradation rate ( Yan et al, 2017 ). PLGA is a kind of synthetic polymer, which is a popular scaffold material in tissue process ( Liu et al, 2021 ; Rocha et al, 2022 ). It has the advantages of controllable degradation rate, high porosity and low toxicity ( Chia and Wu, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Bioinspired Protein/Peptide Loaded 3D Printed PLGA Scaffold Promotes Bone Regeneration

Song

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Background: This study was aimed to investigate the effect of three dimensional (3D)printed poly lactide-co-glycolide (PLGA) scaffolds combined with Gly-Phe-Hyp-Gly-Arg (GFOGER) and bone morphogenetic protein 9 (BMP-9) on the repair of large bone defects.Methods: 3D printing method was used to produce PLGA scaffolds, and the sample was viewed by both optical microscopy and SEM, XRD analysis, water absorption and compressive strength analysis, etc. The rabbits were divided into six groups randomly and bone defect models were constructed (6 mm in diameter and 9 mm in depth): control group (n = 2), sham group (n = 4), model group (n = 4) and model + scaffold group (n = 4 rabbits for each group, 0%,2% and 4%). The rabbits were sacrificed at the 4th and 12th weeks after surgery, and the samples were collected for quantitative analysis of new bone mineral density by micro-CT, histopathological observation, immunohistochemistry and Western blot to detect the protein expression of osteoblast-related genes.Results: This scaffold presented acceptable mechanical properties and slower degradation rates. After surface modification with GFOGER peptide and BMP-9, the scaffold demonstrated enhanced new bone mineral deposition and density over the course of a 12 week in vivo study. Histological analysis and WB confirmed that this scaffold up-regulated the expression of Runx7, OCN, COL-1 and SP7, contributing to the noted uniform trabeculae formation and new bone regeneration.Conclusions: The application of this strategy in the manufacture of composite scaffolds provided extensive guidance for the application of bone tissue engineering.

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Ba/Mg co-doped hydroxyapatite/PLGA composites enhance X-ray imaging and bone defect regeneration

Abstract: Hydroxyapatite (HA) is the most commonly used orthopedic implant material. In recent years, the emergence of cationic doped hydroxyapatite has revealed more possibilities for the biological application of HA. Conventional...

Cited by 30 publications

References 44 publications

Synthesis of Ce/Gd@HA/PLGA Scaffolds Contributing to Bone Repair and MRI Enhancement

Synthesis of Ce/Gd@HA/PLGA Scaffolds Contributing to Bone Repair and MRI Enhancement

A Facile Composite Strategy to Prepare a Biodegradable Polymer Based Radiopaque Raw Material for “Visualizable” Biomedical Implants

Bioinspired Protein/Peptide Loaded 3D Printed PLGA Scaffold Promotes Bone Regeneration

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