Babao Dan induces gastric cancer cell apoptosis via regulating MAPK and NF-κB signaling pathways

Shang, Haixia; Cao, Zhiyun; Zhao, Jinyan; Guan, Jianhua; Liu, Jianxin; Peng, Jun; Chen, Youqin; Sferra, Thomas J.; Sankararaman, Senthilkumar; Lin, Jiumao

doi:10.1177/0300060519867502

Cited by 20 publications

(19 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a relatively stable enzyme, LDH is released from the cell into the culture medium, which can form a visual color reaction. Therefore, the cytotoxicity of PB2 can be quantitatively analyzed by measuring the LDH activity in the culture medium [ 27 ]. A recent study showed that PB2 gallate suppressed glycolytic enzyme of activated T cells by reducing LDH expression at the posttranscriptional level [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Procyanidin B2 induces apoptosis and autophagy in gastric cancer cells by inhibiting Akt/mTOR signaling pathway

Tian

et al. 2021

BMC Complement Med Ther

View full text Add to dashboard Cite

Background Procyanidin B2 (PB2), a unique component of the grape seed and other medicinal plants. PB2 has shown wide anticancer activity in various human cancer cells. However, it remains unclear about the biological effects and associated mechanisms of PB2 on gastric cancer cells. Methods Cell proliferation was measured by CCK8 assay, and cellular lactate dehydrogenase (LDH) release was measured in the culture medium. Cellular apoptosis was observed via TUNEL staining assay and measured by caspase-3 and -9 activities. Autophagy was observed by LC3 staining. Western blot analysis was performed to verify autophagy-associated proteins (Beclin1 and Atg5) and Akt-mTOR pathway. Results PB2 reduced the viability of BGC-823 and SGC-7901 cells in a concentration-dependent manner. Furthermore, PB2 induced increased apoptosis rate of gastric cancer cells and enhanced caspase-3 and -9 activities. Simultaneously, PB2 triggered autophagy in gastric cancer cells, with enhanced LC3 staining and increased expression of Beclin1 and Atg5, while the inhibition of autophagy by 3-MA reversed the PB2-induced suppression on cell viability. In addition, PB2 significantly decreased p-Akt and p-mTOR protein expression of gastric cancer cells. Conclusion PB2 exerts anti-proliferative and apoptotic effects and induces autophagy by modulating Akt/mTOR signaling pathway. PB2 may be developed as a potential therapeutic drug for gastric cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Procyanidin B2 induces apoptosis and autophagy in gastric cancer cells by inhibiting Akt/mTOR signaling pathway

Tian

et al. 2021

BMC Complement Med Ther

View full text Add to dashboard Cite

show abstract

“…BBD, a traditional Chinese medicine formula, has the characteristics of a multi-component, multi-target therapy and is widely used in cancer treatment. Preliminary studies have reported that BBD has been widely used to treat patients with various cancers with noted prevention of complications [13,16]. Wang Q et al found that BBD can inhibit cell growth by inducing autophagy through PI3K/AKT/mTOR signaling and enhancing the anti-tumor effect of cisplatin in non-small cell lung cancer (NSCLC) cells.…”

Section: Discussionmentioning

confidence: 99%

“…Many clinical and experimental studies have demonstrated signi cant antitumor activity by BBD. In addition, these studies highlighted BBD's promising clinical e cacy during posttumor chemotherapy and use as an adjuvant therapy for various cancers, including GC [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Babao dan promotes apoptosis of cisplatin (DDP)-resistant gastric cancer cell line SGC-7901/DDP by inducing autophagy through PI3K/AKT/mTOR pathway modulation

Zhao¹,

W²,

Peng³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Multidrug resistance (MDR) is a critical reason of cancer chemotherapy failure. Babao dan (BBD) is a classical and famous traditional Chinese patent medicine, which has been reported to has anti-gastric cancer activity. However, the roles and molecular mechanisms of the reversal of MDR of gastric cancer by BBD have not been well described until now. Methods: SGC-7901 and SGC-7901/DDP cells were used in this study, and drug resistance and evaluation of the reversal effect of BBD was determined using MTT assays in SGC7901/DDP cells. Doxorubicin (DOX) and Rhodamin123 (Rho123) staining was performed to assess BBD effects on drug accumulation and efflux of drug-resistant gastric cancer cells. Cell apoptosis was directly assessed using DAPI staining. Apoptotic and dead cells were detected by flow cytometry after staining with Annexin V-FITC and propidium iodide (PI). Cyto-ID assays were performed to examine cellular autophagy. Changes in cell protein expression of ABCB1, ABCC1, ABCG2, Bax, Bcl-2, caspase-3, cleaved-caspase-3, LC3, p62, Beclin1 and the PI3K/AKT/mTOR pathway were detected by Western blot. Inhibition of autophagy with 3-MA, chloroquine (CQ) and PI3K antagonist (LY294002) or agonist (740Y-P) , uncovered a role for the potentially downregulated signaling pathway, PI3K/AKT/mTOR.Results: The SGC7901/DDP cell line exhibited multi-drug resistance to DDP, DOX and 5-fluorouracil (5-FU) and the drug resistant index (RI) of DDP, DOX and 5-FU were 1.86, 1.50 and 47.70, respectively. BBD reversed the MDR of SGC7901/DDP cells by increasingDOX accumulation, reducing Rh123 efflux and down-regulating the expression of ABCB1, ABCC1, ABCG2. Furthermore, BBD induced apoptosis in SGC7901/DDP cells through regulating caspase-3, cleaved-caspase-3, Bax and Bcl-2. Moreover, BBD induced autophagy in DDP-resistant gastric cancer cells via regulating p62, LC3 and Beclin1. Pathway analyses suggested BBD may inhibit PI3K/AKT/mTOR pathway activity and subsequent autophagy induction. Conclusions: BBD may reverse the MDR of gastric cancer cells, and promote autophagic death via inactivation of the PI3K/AKT/mTOR signaling pathway.

show abstract

“…Furthermore, BBD has been demonstrated to have significant anti-tumor effects. In particular, BBD was reported to induce cell apoptosis of gastric cancer through regulating MAPK and NF- κ B signaling pathways ( 5 ). Liu et al.…”

Section: Introductionmentioning

confidence: 99%

BaBao Dan Suppresses Tumor Growth of Pancreatic Cancer Through Modulating Transcriptional Reprogramming of Cancer-Related Genes

et al. 2020

View full text Add to dashboard Cite

Background and Aims: Pancreatic ductal adenocarcinoma (PDAC) is one of refractory malignancies without efficient therapeutics. Babao Dan (BBD) was partially effective to suppress tumor growth of PDAC in clinical practice. However, the molecular mechanisms were unclear. Methods: We established PDAC mice models and treated them with BBD through intragastric administration. Treatment and control groups were then subjected to highthroughput RNA sequencing. We presented the transcriptional changes upon BBD treatment by using computational analysis comparing BBD treatment and control groups. Functional enrichment analysis was employed to investigate the biological processes or pathways that BBD modulates. Results: BBD treatment showed strong suppression on tumor growth of PDAC, even stronger than Gemcitabine. Through differential analysis comparing BBD treatment and control groups, we identified 638 up-regulated and 259 down-regulated genes in the BBD treatment group. BBD was found to activate tumor suppressor genes, such as MTUS1, PDGFB, SOD3, and UCHL1. Furthermore, we revealed that BBD treatment inhibited cancer-related pathways and elevated activities of metabolism-related processes. The BBD-modulated metabolic genes were further showed to be associated with patient survival in an independent cohort with pancreatic cancer. Conclusion: BBD repressed the tumor growth of PDAC. BBD treatment modulated expression of cancer-related genes in PDAC. BBD suppressed cancer-related pathways

show abstract

Babao Dan induces gastric cancer cell apoptosis via regulating MAPK and NF-κB signaling pathways

Cited by 20 publications

References 36 publications

Procyanidin B2 induces apoptosis and autophagy in gastric cancer cells by inhibiting Akt/mTOR signaling pathway

Procyanidin B2 induces apoptosis and autophagy in gastric cancer cells by inhibiting Akt/mTOR signaling pathway

Babao dan promotes apoptosis of cisplatin (DDP)-resistant gastric cancer cell line SGC-7901/DDP by inducing autophagy through PI3K/AKT/mTOR pathway modulation

BaBao Dan Suppresses Tumor Growth of Pancreatic Cancer Through Modulating Transcriptional Reprogramming of Cancer-Related Genes

Contact Info

Product

Resources

About