Bacillary growth, interleukin 2 production defect, and specific antibody secretion governed by different genetical factors in mice infected subcutaneously with Mycobacterium lepraemurium

Hoffenbach, A; Bach, Marie‐Anne

doi:10.1016/0008-8749(86)90421-1

Cited by 5 publications

(4 citation statements)

References 30 publications

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“…Under certain experimental or clinical conditions, such infections may produce the same kinds of immunologic impairment that we observed in our malnourished guinea pigs. Thus, infection of mice with high doses of M. bovis BCG (4, 30) or M. lepraemurium (10,11) was found to impair significantly the ability of splenocytes to make IL-2 in response to mitogenic or antigenic stimuli. Patients with either leprosy or active tuberculosis, whose peripheral blood lymphocytes were hyporesponsive to mycobacterial antigens in vitro, were found to exhibit an IL-2 production defect (9,28).…”

Section: Discussionmentioning

confidence: 99%

“…Under certain experimental conditions, mice infected with Mycobacterium bovis BCG exhibit reduced tuberculin (purified protein derivative [PPD]) hypersensitivity and depressed PPD-induced lymphoproliferation associated with a reduction in IL-2 production in vitro by stimulated cells (3, 4, 30). Mice infected with Mycobacterium lepraemurium were found to have a similar IL-2 defect when their splenocytes were cultured with a T-cell mitogen, concanavalin A (ConA) (10,11). In patients with leprosy or tuberculosis, impaired cellular responses to mycobacterial antigens in vitro have been accompanied by significant decreases in IL-2 production and defective IL-2 receptor expression (9,22,25,28).…”

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confidence: 99%

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Dietary protein deficiency and Mycobacterium bovis BCG affect interleukin-2 activity in experimental pulmonary tuberculosis

et al. 1989

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Inbred strain 2 guinea pigs were vaccinated with Mycobacterium bovis BCG or were left unvaccinated. They were maintained for 6 weeks on defined, isocaloric diets containing either 30% (control animals) or 10% (animals receiving low protein) ovalbumin as the sole protein source. Animals were challenged by the respiratory route with a low dose of virulent M. tuberculosis H37Rv and killed 4 weeks later. Proteinmalnourished animals were not protected by previous vaccination with BCG. Lymphocytes isolated from various tissues were tested in vitro for proliferative responses to mitogen (concanavalin A) and antigen (purified protein derivative [PPD]), production of interleukin-2 (IL-2), and response to exogenous recombinant IL-2 (rIL-2). Protein-malnourished guinea pigs responded only weakly to PPD skin tests, and their blood and lymph node lymphocytes exhibited impaired proliferation when cultured with PPD in vitro. IL-2 levels were consistently low in cultures of stimulated blood and spleen lymphocytes from protein-deprived animals. BCG vaccination of nutritionally normal guinea pigs, on the other hand, induced significantly more IL-2 production by PPDand concanavalin A-stimulated lymphocytes. The addition of exogenous mouse rIL-2 (40 and 80 U/ml) in vitro to PPD-stimulated blood and lymph node cells from nonvaccinated, protein-deprived guinea pigs resulted in no improvement of the proliferative response. Previous vaccination of malnourished guinea pigs did not consistently enhance the response of PPD-stimulated lymphocytes to added rIL-2. Dietary protein deficiency and BCG vaccination appear to modulate antigen-driven cellular immunity in animals with tuberculosis by altering the production of, and the response to, IL-2 by PPD-stimulated lymphocytes.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Dietary protein deficiency and Mycobacterium bovis BCG affect interleukin-2 activity in experimental pulmonary tuberculosis

et al. 1989

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“…Glynn & Plant, 1982;Hoffenbach, Lagrange & Bach. 1984, 1985: Skamene et at., 1984Hoffenbach & Bach. 1986).…”

Section: Introductionunclassified

Early accumulation of suppressor cell precursors in the spleen of Mycobacterium lepraemurium-infected mice and analysis of their in vitro-induced maturation

Lemieux

Gosselin

Lusignan

et al. 1990

Clinical and Experimental Immunology

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SUMMARYSpleen cells hat^ested from tnice infected intraperitoneally with M. lepraemurium 11 17 weeks prior to harvest acquired the capacity lo inhibit concanavalin A (Con A) induced proliferation of normal spleen cells when precullured for up to 24 h in mitogen-frec medium. The in vitro induced suppressor activity correlated with the length of the prccullure period, the time post-infection and ihc infecting dose. These lindings were interpreted as an indication thai suppressor cell precursors aeeumulated in the spleen of infected mice during the early phase of the disease. The interaction of infectiondependent adherent suppressor eell preeursors and infection-independent, non-adherent regulatory cells is necessary lor the suppressor activity to develop. Both the cells which transmit the inductive signal and the precursor eells which mature into active suppressor eells are radiosensitive, whereas suppressor activity itself is a funetion of radioresistanl adherent cells. Preculture of cells for a short period.before they were coeuttured with Con A-stimulaied normal spleencells. allowed the deteetion of suppressor cells hefore they were deleterious to the infected hosl and also turned out to be a relevatit //; vitro model for characterization of suppressor cell development during M. lepractmirium infection.

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“…Peritoneal exudate cells of two mice were pooled and tested in duplicate. [8,17,26,45], M. bovis BCG [6, 10, 37. 47].…”

Section: Hydrogen Peroxide Production By Peritoneal Macrophages From mentioning

confidence: 99%

Induction of Non‐Specific Immunosuppression in Mice by Mycobacterial Infections and Its Relationship to Macrophage Activation

Appelberg¹,

Soares²,

Ferreira³

et al. 1989

Scand J Immunol

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The development of non-specific immunosuppression during the infection of different strains of mice with three mycobacterial species was evaluated by studying the immune response to a heterologous antigen (sheep red blood cells) and comparing it with the induction of non-specific resistance to a Listeria monocytogenes challenge. It was shown that early (at 15 days) immunosuppression developed in Mycobacterium avium-susceptible mouse strains infected with a high inoculum dose [2.5 x 10(8) colony forming units (CFU)] of virulent M. avium but not in resistant mice infected with a similar inoculum nor in susceptible mice infected by a smaller inoculum dose (2.5 x 10(6) CFU). In the latter case it developed only during the second month of infection and was of smaller magnitude. An inoculum of M. avium of attenuated virulence did not induce immunosuppression. M. lepraemurium induced a late immunosuppression, which occurred when extensive bacterial proliferation had already taken place. The non-pathogenic M. bovis BCG induced immunosuppression in C57BL/6 mice. The results do not establish a correlation between the development of generalized immunosuppression and susceptibility to infection. It could be seen that the early immunosuppression was observed in those situations where there was extensive macrophage activation as shown by the development of non-specific resistance to a listeria challenge. The late immunosuppression was observed when bacterial proliferation was extensive.

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Bacillary growth, interleukin 2 production defect, and specific antibody secretion governed by different genetical factors in mice infected subcutaneously with Mycobacterium lepraemurium

Cited by 5 publications

References 30 publications

Dietary protein deficiency and Mycobacterium bovis BCG affect interleukin-2 activity in experimental pulmonary tuberculosis

Dietary protein deficiency and Mycobacterium bovis BCG affect interleukin-2 activity in experimental pulmonary tuberculosis

Early accumulation of suppressor cell precursors in the spleen of Mycobacterium lepraemurium-infected mice and analysis of their in vitro-induced maturation

Induction of Non‐Specific Immunosuppression in Mice by Mycobacterial Infections and Its Relationship to Macrophage Activation

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