Bacillus amyloliquefaciens Ameliorates Dextran Sulfate Sodium-Induced Colitis by Improving Gut Microbial Dysbiosis in Mice Model

Abstract

Several Bacillus strains exert beneficial effects on the maintenance of intestinal homeostasis and host health. However, whether Bacillus amyloliquefaciens (BA) can improve gut microbial dysbiosis and ameliorate colitis is unknown. Therefore, we conducted the present study to investigate the effects of BA administration on intestinal morphology, inflammatory response, and colonic microbial composition in a mouse model of dextran sulfate sodium (DSS)-induced colitis. Results showed that BA administration significantly ameliorated body weight loss, decreased disease activity index, and improved colonic tissue morphology in DSS-treated mice. In addition, levels of immunoglobulins, as well as pro-inflammatory cytokines, were decreased after BA administration. Importantly, colonic microbiota profiling indicated a significant (p < 0.05) difference in beta-diversity between BA-administrated and DSS-treated mice, according to weighted principal coordinate analysis (PCoA) results. The relative abundance of the Firmicutes genus was increased, whereas that of Bacteroidetes was decreased by BA administration. Furthermore, phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis showed that the most significantly changed pathways between the four groups of mice were carbohydrate, lipid, and amino acid metabolism. In conclusion, our results showed that BA administration has beneficial effects on DSS-induced colitis, suggesting that this strategy might be useful for the treatment of dysbiosis during ulcerative colitis. Further, the changes in metabolism, especially amino acid metabolism, might contribute to the beneficial effects of BA on the amelioration of DSS-induced colitis.

Anti-inflammatory Bifidobacterium strains prevent dextran sodium sulfate induced colitis and associated gut microbial dysbiosis in mice

“…Dysbiosis in microbiota composition leads to aberrant mucosal immunity in the gut as observed in IBD patients 27 . We have observed dysbiosis in the gut bacteria of DSS treated mice, where at the phylum level the abundance of Bacteroidetes and Deferribacters was decreased with DSS treatment, which was similar to the observation by other researchers 28 . Actinobacteria levels were low in control than DSS treated mice but increased abundance was found in interventions with B. longum Bif10, B. longum Bif11 and B. longum Bif16 to the mice, suggesting gut modulating effects.…”

Establishment and Resilience of Transplanted Gut Microbiota in Aged Mice

“…Aged recipient mice of FMT-M or FMT-UM both showed typical symptoms of acute colitis as shown by weight loss (Figure S8A) and diarrhea and blood in feces ( Figure S8B). These disease symptoms slowly resolved after the mice were switched back to normal drinking water, which is the same as what was reported in young and non-transplanted mice (Cao et al, 2018;Melgar et al, 2005;Tang et al, 2018).…”

Cinobufacini ameliorates experimental colitis via modulating the composition of gut microbiota

“…The V3–V4 region of 16S rRNA was amplified with universal primers. Sequencing and data analysis were subsequently performed on an Ion S5 TM XL platform by Novogene (Beijing, China) using a method described previously[17]. Briefly, ≧97% similarity of the sequences were classified as the same OTUs.…”