Bacillus cereus EC9 protects tomato against Fusarium wilt through JA/ET-activated immunity

Pazarlar, Sercan; Madriz-Ordeñana, Kenneth; Thordal‐Christensen, Hans

doi:10.3389/fpls.2022.1090947

Cited by 15 publications

(8 citation statements)

References 76 publications

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“…The data indicated that MM induced defense-related gene expression levels in watermelon roots in comparison with the control. This finding is consistent with previous reports on Bacillus subtilis MBI600, Bacillus cereus EC9, and Bacillus amyloliquefaciens, which trigger the plant's immune system against fungal phytopathogens by inducing some biosynthetic pathways in the plant [9,52,53]. Furthermore, with the inoculation of FON, the expression of defense-related genes including C4H, ClPR3, and LOX were enhanced.…”

Section: Discussionsupporting

confidence: 92%

A Biocontrol Strain of Serratia plymuthica MM Promotes Growth and Controls Fusarium Wilt in Watermelon

Li,

Ma,

et al. 2023

Agronomy

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Fusarium wilt, caused by Fusarium oxysporum f. sp. niveum (FON), is a predominant and devastating soil-borne disease that results in significant yield losses in watermelon cultivation. In this study, a strain MM isolated from the herbage rhizosphere soil, exhibited an inhibition rate of 65.46% against FON, leading to mycelial collapse, atrophy, and deformation. In pot experiments, strain MM effectively controlled Fusarium wilt of watermelon, showing a control efficacy of 74.07%. Through morphological observation and 16S rDNA gene sequencing, strain MM was identified as Serratia plymuthica. Additionally, S. plymuthica MM demonstrated antagonistic activity against eight plant pathogens, indicating that MM had broad-spectrum antifungal activity. The strain also exhibited the ability to synthesize siderophores and indole acetic acid (IAA), both of which are growth-promoting compounds. Moreover, strain MM secreted various extracellular enzymes, including protease, chitinase, β-glucanase, and cellulase. This ability allowed S. plymuthica MM to readily colonize watermelon roots and promote seedling growth. Inoculation with S. plymuthica MM increased the activity of PAL, POD, PPO, and CAT enzymes associated with watermelon defense. Furthermore, qRT-PCR analysis revealed up-regulation of LOX, POD, PAL, ClPR3, and C4H genes, which are related to plant disease resistance. The results indicated that S. plymuthica MM enhances watermelon plants’ resistance to FON by activating the JA, SA, and shikimic acid phenylpropanoid–lignin synthesis pathways. Gas chromatography–mass spectrometry (GC-MS) analysis of S. plymuthica MM culture supernatant identified piperazinedione, pyrrolo[1,2-a]pyrazine-1,4-dione, and octadecenamide as the main antimicrobial substances. Overall, S. plymuthica MM shows promise as a biocontrol agent against Fusarium wilt of watermelon, suggesting its potential for the development of a new biocontrol agent.

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Section: Discussionsupporting

confidence: 92%

A Biocontrol Strain of Serratia plymuthica MM Promotes Growth and Controls Fusarium Wilt in Watermelon

Li,

Ma,

et al. 2023

Agronomy

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“…The t18:0-based sphingolipids specifically facilitate the translocation of glycosylphosphatidylinositol-anchored PDLP5 protein to plasmodesmata and increase accumulation of callose, which lead to elevated resistance to the bacterium Pseudomonas syringae and the fungal-wilt pathogen Verticillium dahlia ( Liu et al., 2020 ). The beneficial rhizobacteria Bacillus proteolyticus OSUB18 and Bacillus cereus EC9 triggered induced systemic resistance and callose deposition in host plants to protect against pathogens ( Pazarlar et al., 2022 ; Yang et al., 2023 ). However, pathogen effectors, such as RxRL3 being secreted by the plant-damaging oomycete Phytophthora brassicae , SECP8 by citrus Huanglongbing bacterium Candidatus Liberibacter asiaticus and XopAP by rice bacterial blight Xanthomonas oryzae , could hamper callose formation ( Tomczynska et al., 2020 ; Liu et al., 2022 ; Shen et al., 2022 ).…”

Section: Diverse Role Of Callose Function In Plant Development and De...mentioning

confidence: 99%

The multifarious role of callose and callose synthase in plant development and environment interactions

Li,

Lin,

et al. 2023

Front. Plant Sci.

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Callose is an important linear form of polysaccharide synthesized in plant cell walls. It is mainly composed of β-1,3-linked glucose residues with rare amount of β-1,6-linked branches. Callose can be detected in almost all plant tissues and are widely involved in various stages of plant growth and development. Callose is accumulated on plant cell plates, microspores, sieve plates, and plasmodesmata in cell walls and is inducible upon heavy metal treatment, pathogen invasion, and mechanical wounding. Callose in plant cells is synthesized by callose synthases located on the cell membrane. The chemical composition of callose and the components of callose synthases were once controversial until the application of molecular biology and genetics in the model plant Arabidopsis thaliana that led to the cloning of genes encoding synthases responsible for callose biosynthesis. This minireview summarizes the research progress of plant callose and its synthetizing enzymes in recent years to illustrate the important and versatile role of callose in plant life activities.

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“…JAs respond to drought stress ( Yao et al., 2022 ; Liu et al., 2021 ) and Fusarium oxysporum infection ( Hernández-Aparicio et al., 2021 ; Pazarlar et al., 2022 ). Our results confirm the expression of SbLOX3 and that the accumulation of MeJA was induced by PEG-simulated drought stress and F. oxysporum in the hair roots of S. baicalensis ( Figures 3A, B ).…”

Section: Discussionmentioning

confidence: 99%

A 13-LOX participates in the biosynthesis of JAs and is related to the accumulation of baicalein and wogonin in Scutellaria baicalensis

Geng

Wang

Zhang³

et al. 2023

Front. Plant Sci.

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Although baicalein and wogonin contents in Scutellaria baicalensis, a traditional Chinese herb, are known to be regulated by jasmonic acid, the exact mechanism by which jasmonic acid regulates the accumulation of baicalein and wogonin remains unclear. In this study, we discovered SbLOX3, a gene encoding 13-lipoxygenase from the roots of S. baicalensis, which plays an important role in the biosynthesis of jasmonic acid. The contents of methyl jasmonate, baicalin, wogonin, and three metabolic intermediates of methyl jasmonate, 13-HPOT, OPDA, and OPC-8, were downregulated in the hair roots of the SbLOX3 RNAi lines. We confirmed that SbLOX3 was induced by drought stress simulated by PEG and Fusarium oxysporum, which subsequently led to changes in the content of MeJA, baicalin, and wogonin. Taken together, our results indicate that a 13-LOX is involved in the biosynthesis of jasmonic acid, and regulates the accumulation of baicalein and wogonin in S. baicalensis roots.

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Bacillus cereus EC9 protects tomato against Fusarium wilt through JA/ET-activated immunity

Cited by 15 publications

References 76 publications

A Biocontrol Strain of Serratia plymuthica MM Promotes Growth and Controls Fusarium Wilt in Watermelon

A Biocontrol Strain of Serratia plymuthica MM Promotes Growth and Controls Fusarium Wilt in Watermelon

The multifarious role of callose and callose synthase in plant development and environment interactions

A 13-LOX participates in the biosynthesis of JAs and is related to the accumulation of baicalein and wogonin in Scutellaria baicalensis

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