Bacillus krulwichiae sp. nov., a halotolerant obligate alkaliphile that utilizes benzoate and m-hydroxybenzoate

Yumoto, Isao; Yamaga, Shingo; Sogabe, Yoko; Nodasaka, Yoshinobu; Matsuyama, Hidetoshi; Nakajima, Kenji; Suemori, Akio

doi:10.1099/ijs.0.02596-0

Cited by 72 publications

(48 citation statements)

References 28 publications

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“…Most moderately halotolerant Bacillus species were isolated from saline areas or marine environments, related regions or materials; in rare cases they were found in non-saline environments (Wainø et al, 1999;Yoon et al, 2003;Yumoto et al, 2003;Vargas et al, 2005). During the course of a study on the culturable aerobic and facultative anaerobic bacterial community present in a soil sample from a ginseng field in Pocheon Province, South Korea, a large number of novel bacterial strains were isolated .…”

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confidence: 99%

Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field

Ten

Baek

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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A Gram-positive, non-motile, endospore-forming bacterial strain, designated Gsoil 420T, was isolated from soil of a ginseng field in Pocheon Province, South Korea, and was characterized, using a polyphasic approach, in order to determine its taxonomic position. The novel isolate consisted of strictly aerobic, rod-shaped cells and was able to grow in medium supplemented with up to 12 % NaCl at 25 °C and pH 6.5–7.0. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 420T fell within the radiation of the cluster comprising Bacillus species and formed a coherent cluster with Bacillus niacini (16S rRNA gene sequence similarity, 98.6 %), Bacillus bataviensis (98.6 %), Bacillus soli (98.3 %), Bacillus drentensis (98.0 %), Bacillus novalis (98.0 %), Bacillus vireti (97.9 %), Bacillus foraminis (97.6 %), Bacillus fumarioli (97.4 %) and Bacillus jeotgali (97.0 %). The levels of 16S rRNA gene sequence similarity with respect to other Bacillus species with validly published names were less than 96.8 %. Strain Gsoil 420T had a genomic DNA G+C content of 44.9 mol% and the predominant respiratory quinone was MK-7. The major fatty acids were anteiso-C15 : 0 (33.9 %), iso-C15 : 0 (24.5 %) and iso-C14 : 0 (19.9 %). These chemotaxonomic results supported the affiliation of strain Gsoil 420T to the genus Bacillus. However, low DNA–DNA relatedness values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation of strain Gsoil 420T from recognized Bacillus species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 420T represents a novel species of the genus Bacillus, for which the name Bacillus pocheonensis sp. nov. is proposed. The type strain is Gsoil 420T (=KCTC 13943T=DSM 18135T).

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confidence: 99%

Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field

Ten

Baek

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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“…Subsequently B. horti (Yumoto et al, 1998), B. okuhidensis (Li et al, 2002), B. krulwichiae (Yumoto et al, 2003), B. decolorationis (Heyrman et al, 2003), B. patagoniensis (Olivera et al, 2005) and B. okhensis (Nowlan et al, 2006) have been added to Bacillus rRNA group 6.…”

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Bacillus plakortidis sp. nov. and Bacillus murimartini sp. nov., novel alkalitolerant members of rRNA group 6

Borchert

Nielsen

Graeber

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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The Gram-positive, alkali- and salt-tolerant marine bacterium strain P203T is described together with its closest phylogenetic neighbour, terrestrial isolate LMG 21005T. Strain P203T was isolated from material from the sponge Plakortis simplex that was obtained from the Sula-Ridge, Norwegian Sea. Strain LMG 21005T was an undescribed strain that was isolated from a church wall mural in Germany. Strains P203T and LMG 21005T were identified as novel alkalitolerant members of the Bacillus rRNA group 6 with a 16S rRNA gene sequence similarity of 99.5 %. The closest described neighbour, Bacillus gibsonii DSM 8722T, showed 99.0 % gene sequence similarity with P203T and 98.8 % similarity with strain LMG 21005T. Despite the high 16S rRNA gene sequence similarity, DNA–DNA cross-hybridization revealed only 25.8–34.1 % similarity amongst the three strains. The DNA G+C contents were 41.1 mol% for strain P203T and 39.6 mol% for strain LMG 21005T. Both strains grew well between pH 7 and pH 11. Strain P203T showed growth at moderate temperatures (from 4 to 30 °C) and in the presence of up to 12 % (w/v) NaCl at pH 9.7, whereas strain LMG 21005T was not salt tolerant (up to 4 % NaCl) and no growth was observed at 4 °C. The major fatty acids of strains P203T, LMG 21005T and the type strain of B. gibsonii were the saturated terminally methyl-branched compounds iso-C15 : 0 (19.8, 15.6 and 28.0 %, respectively) and anteiso-C15 : 0 (57.1, 48.6 and 45.2 %, respectively). Physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains P203T and LMG 21005T from the six related Bacillus species with validly published names and supported the proposal of two novel species, Bacillus plakortidis [type strain P203T (=DSM 19153T=NCIMB 14288T)] and Bacillus murimartini [type strain LMG 21005T (=NCIMB 14102T)].

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“…Evaluation of tolerance to NaCl was performed by inoculating 60 ml of inocula into 6 ml AS medium with NaCl concentrations of 0-25 % (w/v) at pH 9 in test tube. Growth at different pH values was determined in AS liquid medium at 30 u C containing 100 mM NaH 2 PO 4 /Na 2 HPO 4 buffer (pH 7-8) or 100 mM NaHCO 3 /Na 2 CO 3 buffer (pH 9-11) with pH adjusted to pH 5-11 by the measurement of OD 600 (Yumoto et al, 2003). According to Sorokin's point of view, there is no buffering system that can maintain a stable pH of 11 (Sorokin et al, 2008).…”

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Bacillus ligniniphilus sp. nov., an alkaliphilic and halotolerant bacterium isolated from sediments of the South China Sea

Zhu

Tanabe

Xie

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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An alkaliphilic and halotolerant Gram-stain-positive bacterium, which was isolated from sediment samples from the South China Sea, was subjected to a taxonomic study. The isolate, strain L1 T , grew well at a wide range of temperatures and pH values, 10.0-45.0 6C and pH 6-11, with optima at 30 6C and pH 9.0, respectively. The growth of strain L1 T occurred at total salt concentrations of 0-10 % (w/v) with an optimum at 2 % (w/v). Phylogenetic analysis based on 16S rRNA sequence comparison indicated that the isolate represented a member of the genus Bacillus. The strains most closely related to strain L1 T were Bacillus nanhaiisediminis JCM 16507 T , Bacillus halodurans DSM 497 T and Bacillus pseudofirmus DSM 8715 T , with 16S rRNA similarities of 96.5 %, 95.9 % and 95.7 %, respectively. DNA-DNA hybridization of strain L1 T with the type strains of the most closely related species, B. nanhaiisediminis JCM 16507 T , B. halodurans DSM 497 T and B. pseudofirmus DSM 8715 T , showed reassociation values of about 21.7 %, 14.3 % and 13.9 %, respectively. The DNA G+C content of strain L1 T was 40.76 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant cellular fatty acids of strain L1 T were iso-C 14 : 0 and anteiso-C 15 : 0 . The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on the phenotypic and phylogenetic characteristics, it is proposed that strain L1 T (5JCM 18543 T 5DSM 26145 T ) should be classified as the type strain of Bacillus ligniniphilus sp. nov.The genus Bacillus, which already contains more than 200 species, is one of the largest genera in the bacterial kingdom (http://www.bacterio.net/b/bacillus.html). Members of this genus live in a wide range of habitats, such as desert sands, hot spring vents and polar regions (Amoozegar et al., 2009;Gugliandolo et al., 2003; Shi et al., 2011;Sorokin et al., 2008; Zhang et al., 2011a, b). Generally, cells of members of the genus Bacillus are aerobic or facultatively anaerobic, sporeforming, alkaliphilic, halophilic or halotolerant and Gramstain-positive rods. During an investigation of the culturable bacterial community from sediments of the South China Sea from a depth of 100-4000 m, a large number of bacterial strains were isolated. In the present study, a halotolerant alkaliphilic bacterium that was able to utilize lignin as a sole carbon source was isolated, with the aim of studying its degradation mechanisms for the depolymerization of lignin. Based on the results of phenotypic and chemotaxonomic studies and a phylogenetic analysis of 16S rRNA gene sequences, the novel isolate L1 T should be classified as representing a novel species of the genus Bacillus. The reference strains used in this study were type strains belonging to the genus Bacillus including Bacillus nanhaiisediminis JCM 16507 T , Bacillus halodurans DSM 497 T and Bacillus pseudofirmus DSM 8715 T , obtained from the G...

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Bacillus krulwichiae sp. nov., a halotolerant obligate alkaliphile that utilizes benzoate and m-hydroxybenzoate

Cited by 72 publications

References 28 publications

Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field

Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field

Bacillus plakortidis sp. nov. and Bacillus murimartini sp. nov., novel alkalitolerant members of rRNA group 6

Bacillus ligniniphilus sp. nov., an alkaliphilic and halotolerant bacterium isolated from sediments of the South China Sea

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