Bacillus kyonggiensis sp. nov., isolated from soil of a lettuce field

Lee, Sangseob

doi:10.1007/s12275-011-1218-7

Cited by 11 publications

(4 citation statements)

References 17 publications

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“…In addition, the 1500 bp PCR fragment of each strain, HIP1, HIP2, HIP3, and HIO1 in this study, display comparable result with Bacillus sp. NB22, which had a 1463 bp PCR fragment when 27F and 1492R primers were used [31]. The complete sequence (1433 bases) of the 16S rRNA gene of strain SC-N012T allowed it to be described as a member of a novel species of the genus Bacillus , for which the name Bacillus rhizosphaerae sp.…”

Section: Resultsmentioning

confidence: 99%

Production of Biosurfactant Produced from Used Cooking Oil by Bacillus sp. HIP3 for Heavy Metals Removal

et al. 2019

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Heavy metals from industrial effluents and sewage contribute to serious water pollution in most developing countries. The constant penetration and contamination of heavy metals into natural water sources may substantially raise the chances of human exposure to these metals through ingestion, inhalation, or skin contact, which could lead to liver damage, cancer, and other severe conditions in the long term. Biosurfactant as an efficient biological surface-active agent may provide an alternative solution for the removal of heavy metals from industrial wastes. Biosurfactants exhibit the properties of reducing surface and interfacial tension, stabilizing emulsions, promoting foaming, high selectivity, and specific activity at extreme temperatures, pH, and salinity, and the ability to be synthesized from renewable resources. This study aimed to produce biosurfactant from renewable feedstock, which is used cooking oil (UCO), by a local isolate, namely Bacillus sp. HIP3 for heavy metals removal. Bacillus sp. HIP3 is a Gram-positive isolate that gave the highest oil displacement area with the lowest surface tension, of 38 mN/m, after 7 days of culturing in mineral salt medium and 2% (v/v) UCO at a temperature of 30 °C and under agitation at 200 rpm. An extraction method, using chloroform:methanol (2:1) as the solvents, gave the highest biosurfactant yield, which was 9.5 g/L. High performance liquid chromatography (HPLC) analysis confirmed that the biosurfactant produced by Bacillus sp. HIP3 consists of a lipopeptide similar to standard surfactin. The biosurfactant was capable of removing 13.57%, 12.71%, 2.91%, 1.68%, and 0.7% of copper, lead, zinc, chromium, and cadmium, respectively, from artificially contaminated water, highlighting its potential for bioremediation.

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Section: Resultsmentioning

confidence: 99%

Production of Biosurfactant Produced from Used Cooking Oil by Bacillus sp. HIP3 for Heavy Metals Removal

et al. 2019

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“…Since the genus was established in 2020, Robertmurraya currently consists of seven validly-published species according to the List of Prokaryotic names with Standing in Nomenclature (LPSN, https://lpsn.dsmz.de/genus/robertmurraya). Although Robertmurraya type species have been isolated from soil (37, 38, 39), biowaste composting reactors (40), silage (41), and fecal flora (42), our recovered isolate has the highest sequence similarity to the psychrotolerant Robertmurraya beringensis seawater isolate ( Supplemental Table S4 ; (43)).…”

Section: Resultsmentioning

confidence: 98%

“…Since the genus was established in 2020, Robertmurraya currently consists of seven validly-published species according to the List of Prokaryotic names with Standing in Nomenclature (LPSN, https://lpsn.dsmz.de/genus/robertmurraya). Although Robertmurraya type species have been isolated from soil (37,38,39), biowaste composting reactors (40), silage (41), and fecal flora (42), our recovered isolate has the highest sequence similarity to the psychrotolerant Robertmurraya beringensis seawater isolate (Supplemental Table S4;…”

Section: The Majority Of Antarctic Sediment-associated Bacteria Were ...mentioning

confidence: 99%

Culture-dependent identification of rare marine sediment bacteria from the Gulf of Mexico and Antarctica

Kennedy,

Atkinson,

Tubbs

et al. 2024

Preprint

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Laboratory-viable cultivars of previously uncultured bacteria further taxonomic understanding. Despite many years of modern microbiological investigations, the vast majority of bacterial taxonomy remains uncharacterized. While many attempts have been made to decrease this knowledge gap, culture-based approaches parse away at the unknown and are critical for improvement of both culturing techniques and computational prediction efficacy. To this end of providing culture-based approaches, we present a multi-faceted approach to recovering marine environmental bacteria. We employ combinations of nutritional availability, inoculation techniques, and incubation parameters in our recovery of marine sediment-associated bacteria from the Gulf of Mexico and Antarctica. The recovered biodiversity spans several taxa, with 16S-ITS-23S rRNA gene-based identification of multiple isolates belonging to rarer genera increasingly undergoing phylogenetic rearrangements. Our modifications to traditional culturing techniques have not only recovered rarer taxa, but also resulted in the recovery of biotechnologically promising bacteria. Together, we propose our stepwise combinations of recovery parameters as a viable approach to decreasing the bacterial knowledge gap.

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“…Spore formation of strains U13 T and U14 was determined by incubating on modified Schaeffer's medium [0.1 % KCl, 0.01 % MgCl 2 , 1.0 mM Ca(NO 3 ) 2 , 0.01 mM MnCl 2 , 0.001 mM FeSO 4 , 8.0 g nutrient broth l − 1 ] (Dong & Lee, 2011; Kempf et al , 2005) for 7 days. Spores were stained with Schaeffer–Fulton stain (Schaeffer & Fulton, 1933) and observed with a phase-contrast microscope (Nikon light microscope; × 1000 magnification).…”

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confidence: 99%

Tumebacillus luteolus sp. nov., isolated from soil

Her

Srinivasan

Lee

2015

International Journal of Systematic and Evolutionary Microbiology

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Two strains of Gram-stain-positive, aerobic, spore-forming and rod-shaped bacteria, designated U13T and U14, were isolated from soil of the Ukraine. Comparative analysis of the 16S rRNA gene sequences indicated that these strains belong to the genus Tumebacillus, with the highest 16S rRNA gene sequence similarity with Tumebacillus ginsengisoli Gsoil 1105 T (95.48 % and 95.49 %, respectively). Strains U13 T and U14 had iso-C 15 : 0 and summed features 1 and 4 as the main fatty acids, and were able to grow at pH ranging from pH 5.0 to 9.0 (optimum pH 6.0-7.0), temperatures ranging from 25 to 42 8C (optimum 28-37 8C) and with 0-1 % (w/v) NaCl (optimum 0 %, w/v) on R2A agar medium. Chemotaxonomic data revealed that the cell-wall peptidoglycan type of the two strains was type A1c (meso-diaminopimelic acid The genus Tumebacillus, containing Gram-stain-positive, aerobic, rod-shaped, non-motile bacteria, was proposed by Steven et al. (2008). To date, three species of the genus Tumebacillus have been recognized. These species inhabit various environments, such as permafrost, ginseng fields and wastewater. Novel bacteria, designated strains U13 T and U14, were isolated from Ukrainian soil, and 16S rRNA gene sequence analysis indicated that these strains could be included in the order Bacillales and the family Alicyclobacillaceae. In this study, we characterized the two novel strains, designated U13T and U14. The phenotypic, chemotaxonomic and phylogenetic data collected proved that these isolates belong to the genus Tumebacillus. According to the data, these strains should be classified as representatives of a novel species in the genus Tumebacillus. Strains U13T and U14 were isolated from soil in the Ukraine (GPS, 488 229 26.110 N 318 109 52.200 E). Soil samples were diluted in 0.85 % NaCl solution up to a factor of 10 26

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Bacillus kyonggiensis sp. nov., isolated from soil of a lettuce field

Cited by 11 publications

References 17 publications

Production of Biosurfactant Produced from Used Cooking Oil by Bacillus sp. HIP3 for Heavy Metals Removal

Production of Biosurfactant Produced from Used Cooking Oil by Bacillus sp. HIP3 for Heavy Metals Removal

Culture-dependent identification of rare marine sediment bacteria from the Gulf of Mexico and Antarctica

Tumebacillus luteolus sp. nov., isolated from soil

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