Bacillus nanhaiensis sp. nov., isolated from an oyster

Abstract

A novel Gram-stain-positive, slightly halophilic, facultatively alkaliphilic, catalase-positive, oxidasenegative, endospore-forming, motile, rod-shaped, aerobic bacterium, designated strain JSM 082006 T , was isolated from an oyster collected from Naozhou Island in the South China Sea. The isolate grew in 0-18 % (w/v) NaCl (optimum, 0.5-4.0 %), at pH 6.0-10.5 (optimum, pH 8.0) and at 15-45 6C (optimum, 30 6C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C 15 : 0 , iso-C 15 : 0 and C 16 : 0 . Strain JSM 082006T contained MK-7 as the predominant respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The genomic DNA G+C content was 40.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 082006 T should be assigned to the genus Bacillus and that it was most closely related to the type strains of Bacillus barbaricus (sequence similarity 99.1 %) and Bacillus arsenicus (97.5 %), followed by those of Bacillus rigui (96.6 %) and Bacillus solisalsi (96.1 %). Phylogenetic analysis, DNA-DNA relatedness values, phenotypic characteristics and chemotaxonomic data support the view that strain JSM 082006 T represents a novel species of the genus Bacillus, for which the name Bacillus nanhaiensis sp. nov. is proposed; the type strain is JSM 082006 T (5DSM 23009 T 5KCTC 13712 T ).Halophilic, halotolerant, alkaliphilic and/or alkalitolerant species of the genus Bacillus are widely distributed throughout various types of saline environments (Ash et al., 1991;Nielsen et al., 1994;Ventosa et al., 1998;Arahal & Ventosa, 2002;Romano et al., 2005; Lim et al., 2006a, b;Carrasco et al., 2007;Yumoto, 2007;Aino et al., 2008;Chen et al., 2009e;Liu et al., 2009) and these bacteria have attracted increasing interest, attributable to their ability to grow under extreme conditions as well as to the potential use of their enzymes in biotechnological applications (Horikoshi, 1999;Margesin & Schinner, 2001;Nogi et al., 2005;Krulwich et al., 2007). During an investigation of the diversity of the microbial population of invertebrates inhabiting the South China Sea (Chen et al., 2009a, b, c, d, e; Huang et al., 2009; Xiao et al., 2009), a slightly halophilic, facultatively alkaliphilic, endospore-forming, Gram-stain-positive bacterium, designated strain JSM 082006 T , was isolated from an oyster collected from Naozhou Island, China. Data from the present taxonomic study indicate that this strain represents a novel species of the genus Bacillus. Strain JSM 082006T was isolated from homogenates of an oyster by plating 1 : 10 serial dilutions of the sample on marine agar 2216 (MA; Difco) cultivated at 30 uC for 2 weeks. After primary isolation, the strain was purified by repeated streaking and subculturing on nutrient agar (NA; Atlas, 1993) plates (4-5 times) and examining the cultures by light microscopy. The isolate was preserved both on NA slants at 4 u C and as 20 % (v/...

Fictibacillus phosphorivorans gen. nov., sp. nov. and proposal to reclassify Bacillus arsenicus , Bacillus barbaricus , Bacillus macauensis , Bacillus nanhaiensis ,

“…These relatively low DNA-DNA hybridization values along with high 16S rRNA gene sequence similarities had been reported previously by Chen et al (2011). The DNA G+C mol% content of strain Ca7 T was determined as described …”

“…bacterio.org) isolated from various sources, among them are halophilic, halotolerant, alkaliphilic and/or alkalitolerant species (Ash et al, 1991;Nielsen et al, 1994;Ventosa et al, 1998;Arahal & Ventosa, 2002;Romano et al, 2005;Lim et al, 2006 a, b;Carrasco et al, 2007;Yumoto, 2007;Aino et al, 2008;Chen et al, 2009Chen et al, , 2011Liu et al, 2009). It has been already pointed out by Chen et al (2011) that some of these bacteria may reveal some interesting properties, e.g. the potential use of their enzymes in biotechnological applications (Horikoshi, 1999;Margesin & Schinner, 2001;Nogi et al, 2005;Krulwich et al, 2007).…”

Microbiological investigations on the water of a thermal bath at Budapest

“…Many of the detected taxa are able to utilize amino acids as sole carbon source, e.g., Kinneretia asaccharophila [23], Acinetobacter baumanni [24], Fictibacillus nanhaiensis [25], Pannonibacter phragmitetus [26], Phenylobacterium falsum [27], Rhizobium alkalisoli [20], Porphyrobacter colymbi [28], and Pseudomonas knackmussii [29]. These microbes may also utilize the degradation products of proteins of the human epithelial surface or human perspiration can occur in the pool water.…”

Bacillus crassostreae sp. nov., isolated from an oyster (Crassostrea hongkongensis)

“…Members of the genus Bacillus are generally characterized to be Gram-positive, rod-shaped, endospore-forming, aerobic or facultatively anaerobic, and have menaquinone-7 (MK-7) as the major respiratory quinone, and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids and iso-/anteiso-C 15 : 0 as the predominant cellular fatty acids (Priest et al , 1988; Ash et al , 1991; Kämpfer, 1994; Täubel et al , 2003; Ahmed et al , 2007; Zhang et al , 2010; Kosowski et al , 2014). During an investigation of the diversity of the microbial population of invertebrates inhabiting Naozhou Island (20° 52–56′ N 110° 33–38′ E) in the South China Sea (Huang et al , 2009; Xiao et al , 2009, 2013), a number of novel taxa have been isolated (Chen et al , 2009a, b, c, 2010, 2011a, b, c). Here we describe the taxonomic properties of an endospore-forming, Gram-stain-positive bacterium, designated strain JSM 100118 T , which was isolated from an oyster ( Crassostrea hongkongensis ) collected from the tidal flat of Naozhou Island.…”