Bacillus sp. Acting as Dual Role for Corrosion Induction and Corrosion Inhibition with Carbon Steel (CS)

Abstract

Present work investigated the role of five different bacteria species as a corrosion inducer as well as corrosion inhibitor with carbon steel (CS). We observed the ability of different bacteria species on the metal surface attachment, biofilm formation, and determined Peroxidase, Catalase enzyme activity in the detached biofilm from the CS surface. We found that each strain has diverse conduct for surface attachment like DS1 3.3, DS2 2.5, DS3 4.3, DS4 4.0, and DS5 4.71 log cfu/cm2 and for biofilm 8.3 log cfu/cm2. The enzyme Peroxidase, Catalase was found in huge concentration inside the biofilm Peroxidase was maximum for DS4 36.0 U/ml and least for DS3 19.54 U/ml. Whereas, Catalase was highest for DS4, DS5 70.14 U/ml and least 57.2 U/ml for DS2. Scanning electron microscopy (SEM) was conducted to examine the biofilm and electrochemical impedance spectroscopy (EIS) were utilized to observe corrosion in the presence of bacteria. The electrochemical results confirmed that DS1, DS3, DS4, and DS5 strains have statistically significant MIC-factors (Microbially Influenced Corrosion) of 5.46, 8.51, 2.36, and 1.04, while DS2 protective effect factor of 0.89. Weight reduction results with carbon steel likewise supports that corrosion was initiated by DS1 and DS3, while DS2 and DS5 have no any impact though with DS4 we watched less weight reduction however assumed no role in the corrosion. We established the relation of Peroxidase enzyme activity of the isolates. DS1, DS3 and having Peroxidase in the range 22.18, 19.54 U/ml which induce the corrosion whereas DS2 and DS5 having 28.57 and 27.0 U/ml has no any effect and DS4 36 U/ml has inhibitory effect, increasing concentration inhibiting the corrosion. For Catalase DS1, DS3 have 67.28, 61.57 U/ml which induce corrosion while DS2 and DS5 57.71 and 59.14 U/ml also has no effect whereas DS4 70.14 U/ml can inhibit corrosion. Results clearly express that in a specific range both enzymes can induce the corrosion. Our goals are to pursuit and locate the potential role of the enzyme in corrosion induction and inhibition. There is still further work is proceeded for the more profound perception.

Metal-Adapted Bacteria Isolated From Wastewaters Produce Biofilms by Expressing Proteinaceous Curli Fimbriae and Cellulose Nanofibers

“…Thus, protein and/or polysaccharide produced by E. asburiae ENSD102, uncultured Vitreoscilla sp. ENSG301, and A. lwoffii ENSG302 in response to different concentrations of Cu, Zn, Pb, Ni, and Cr could sequester the toxic metal ions, giving to the bacteria the time required for adaptation thus driving to the physiological or metabolic changes necessary for eliminating the toxic effect of these metals, i.e., expression of enzymes and transporters for pumping out the metal or metal-binding proteins ( Letelier et al, 2010 ; Mindlin et al, 2016 ; Nocelli et al, 2016 ; Karn et al, 2017 ). Thus, these bacterial strains might be an attractive biotechnological tool for bioremediation of toxic heavy metals from wastewaters.…”

Effect of Dissolved Oxygen Concentration on the Microbiologically Influenced Corrosion of Q235 Carbon Steel by Halophilic Archaeon Natronorubrum tibetense

“…MIC has become one of the main threats in the field of oil and gas industry, water treatment industry, and marine engineering, which brings huge economic losses and potential safety hazards (Li et al., 2017). For a long time, numerous research works have been focused on sulfate reducing bacteria (SRB), iron oxidizing bacteria (IOB), sulfur oxidizing bacteria (SOB), and other bacteria (Enning and Garrelfs, 2014; Huber et al., 2016; Liang et al., 2016; Liu et al., 2016; Xu et al., 2016, 2018; Jia et al., 2017; Karn et al., 2017; Dou et al., 2018; Liu and Cheng, 2018). For the ubiquitous microorganisms in the natural environment, such as SRB, their MIC mechanisms have been studied in-depth, and comprehensive corrosion mechanisms have been well established (Venzlaff et al., 2013; Rajala et al., 2015; Li et al., 2018; Jia et al., 2019).…”

Breakthroughs in the discovery and use of different peroxidase isoforms of microbial origin

“…The biofilms detached from the coupons were assayed for peroxidase activity. The highest peroxidase were observed from DS4 (± 36U/ml) and the least from DS3 (±19.54 U/ml) with biofilms and almost no peroxidase in the control samples [55] . It was found that peroxidase and catalase were involved in the corrosion and the bacterial strains with the most MIC also produced the highest concentration of these enzymes.…”

“…Microbially influenced corrosion (MIC) is a physical, chemical and microbiological process in which corrosion and electrochemical processes are initiated, facilitated or accelerated by the dynamic support of microorganisms [55] . Microbes derive their energy by oxidizing and reducing a variety of chemical compounds, and they can change their metabolic activities and release various proteins based on the present conditions, of which in some cases lead to MIC.…”

“…A study [55] conducted to investigate microorganisms isolated from the corroded carbon steel sample that had been kept in sea water for about 2 years showed the formation of biofilms in all the isolates on carbon steel coupons. The biofilms detached from the coupons were assayed for peroxidase activity.…”